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Effect of Mother Trees and Dark Culture Condition Affecting on Somatic Embryogenesis of Liriodendron tulipifera L.  

Son, Seog-Gu (Warm-temperature Forest Research Center, Korea Forest Research Institute)
Moon, Heung-Kyu (Div. Biothechnology, Korea Forest Research Institute)
Kim, Yong-Wook (Div. Biothechnology, Korea Forest Research Institute)
Kim, Ji-Ah (Div. Biothechnology, Korea Forest Research Institute)
Publication Information
Journal of Korean Society of Forest Science / v.94, no.1, 2005 , pp. 39-44 More about this Journal
Abstract
An effective micropropagation system for Liriodendron tulipifera L via somatic embryogenesis was established using immature seeds. Immature seeds from five individual trees were bisected longitudinally and cultured on two basal media (MS and B5) containing different combinations of 2,4-D and TDZ to induce callus and embryogenic tissue under light ($40{\mu}mol\;m^{-2}s^{-1}$, 16 hr/day) or complete darkness at $25{\pm}2^{\circ}C$. There was no distinctive difference on callus and embryogenic tissue induction between the two basal media with PGRs. Optimum culture medium appeared to be MS medium supplemented with 1.0mg/L 2,4-D and 0.01mg/L TDZ plus 3% sucrose. Nonembryogenic callus induction rate was not significantly different among the genotypes. However, However, the embryogenic callus induction frequency differed greatly by the genotypes ranging from 55% to 72% when cultured in the dark. Generally, the cultures maintained in the dark tended to show normal somatic embryo development as well as embryogenic tissue formation and this was confirmed by histological examination. Above results suggest that a proper selection of mother tree and dark culture condition are necessary to optimize somatic embryogenesis system of Liriodendron tulipifera.
Keywords
yellow poplar; somatic embryo maturation and conversion; dark culture; histology;
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