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http://dx.doi.org/10.6109/jkiice.2019.23.3.261

Design of a customizable fluorescence detection system for fluorescently labeled tumor cells  

Cho, Kyoungrae (Department of Medical IT Convergence Engineering, Kumoh National Institute of Technology)
Seo, Jeong-hyeok (Department of Medical IT Convergence Engineering, Kumoh National Institute of Technology)
Choe, Se-woon (Department of Medical IT Convergence Engineering, Kumoh National Institute of Technology)
Publication Information
Journal of the Korea Institute of Information and Communication Engineering / v.23, no.3, 2019 , pp. 261-266 More about this Journal
Abstract
Flow cytometry is an electrical detection system that provides precise and diverse optical properties to cells and micro particles. Flow cytometry, which provides multidimensional information including cell size and granularity through light scattering and fluorescence emission generated by the induction of light of a specific wavelength to the fluorescently treated cells or micro particles, plays an important role in biomedical and biophysical fields. However, it has some drawbacks such as high cost, size of the instrument and limitation in selecting fluorescent dyes. Therefore, in this paper, a low cost compact fluorescent detection system is developed using light-emitting diode and microcontroller. The proposed fluorescence detection system has a replaceable the light source/fluorescence filter/photodetector and constructed by 3D printer, so that the user can design a customized system according to the selected fluorescent dyes. The fluorescence intensity was measured by varying the number of fluorescently labeled cells, and the measured intensities showed a high linearity within the tested concentration ranges.
Keywords
Fluorescence detection; Light-emitting diode; microcontroller; flow cytometry; 3D printer;
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Times Cited By KSCI : 2  (Citation Analysis)
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