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http://dx.doi.org/10.6109/jkiice.2014.18.2.482

Study on PPARγ-dependent adipogenesis regulation by testosterone using transient transfection assays  

Jeong, Sunhyo (Department of Science Management, Mokwon University)
Publication Information
Journal of the Korea Institute of Information and Communication Engineering / v.18, no.2, 2014 , pp. 482-487 More about this Journal
Abstract
This study is to investigate the effects of testosterone on adipogenesis and its molecular mechanism using RT-PCR analysis and transient transfection assays. Castrated(CAST) mice treated with testosterone had lower white adipose tissue weights and expression of adipocyte-specific genes($PPAR{\gamma}$ and aP2) than CAST control mice. Consistent with the in vivo data, testosterone treatment inhibited triglyceride accumulation and expression of adipocyte-specific genes($PPAR{\gamma}$ and aP2) in differentiated 3T3-L1 cells compared with control group. Testosterone-activated androgen receptor(AR) repressed the luciferase reporter gene activity induced by $PPAR{\gamma}$ transfection. Thus, these results suggest that testosterone downregulates the actions of $PPAR{\gamma}$ on adipogenesis through AR.
Keywords
testosterone; $PPAR{\gamma}$; adipogenesis; transient transfection assays;
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