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http://dx.doi.org/10.13048/jkm.22026

Anti-inflammatory Effects of Myrrh Ethanol Extract on Particulate Matter-induced Skin Injury  

Young Hee Jung (Dept. of Third Medicine, Professional Graduate School of Korean Medicine, Wonkwang University)
Yeun Wha Roh (Dept. of Korean Pathology, College of Korean Medicine, Wonkwang University)
Myongsoo Chong (Dept. of Preventive Medicine, College of Korean Medicine & Research Center of Traditional Korean Medicine, Wonkwang University)
Publication Information
The Journal of Korean Medicine / v.43, no.3, 2022 , pp. 1-15 More about this Journal
Abstract
Objectives: Myrrh have been used as a traditional remedy to treat infectious and inflammatory diseases. However, it is largely unknown whether myrrh ethanol extract could exhibit the inhibitory activities against particulate matter (PM)-induced skin injury on human keratinocytes, HaCaT cells. Therefore, this study was aimed to investigate the inhibitory activity of myrrh ethanol extract on PM-induced skin injury in HaCaT cells. Methods: To investigate the inhibitory effects of myrrh ethanol extract in HaCaT cells, the skin injury model of HaCaT cells was established under PM treatment. HaCaT keratinocyte cells were pre-treated with myrrh ethanol extract for 1 h, and then stimulated with PM. Then, the cells were harvested to measure the cell viability, reactive oxygen species (ROS), pro-inflammatory cytokines including interleukin (IL) 1-beta, IL-6, and tumor necrosis factor (TNF)-𝛼, hyaluronidase, collagen, MMPs. In addition, we examined the mitogen activated protein kinases (MAPKs) and inhibitory kappa B alpha (I𝜅-B𝛼) as inhibitory mechanisms of myrrh ethanol extract. Results: The treatment of myrrh ethanol extract inhibited the PM-induced cell death and ROS production in HaCaT cells. In addition, myrrh ethanol extract treatment inhibited the PM-induced elevation of IL-1beta, IL-6, and TNF-𝛼. Also, myrrh ethanol extract treatment inhibited the increase of hyaluronidase, MMP and decrease of collagen. Furthermore, myrrh ethanol extract treatment inhibited the activation of MAPKs and the degradation of I𝜅-B𝛼. Conclusions: Our result suggest that treatment of myrrh ethanol extract could inhibit the PM-induced skin injury via deactivation of MAPKs and nuclear factor (NF)-𝜅B in HaCaT cells. This study could suggest that myrrh ethanol extract could be a beneficial agent to prevent skin damage or inflammation.
Keywords
Myrrh; Myrrh ethanol extract; Particulate matter; Keratinocyte; Skin; Inflammation;
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