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http://dx.doi.org/10.13048/jkm.20043

Immunostimulatory effects of the Pueraria lobata flower extract via MAPK signaling in RAW264.7 cells  

Kim, Ki-tae (Dept. of Internal Medicine, College of Korean Medicine, Semyung University)
Publication Information
The Journal of Korean Medicine / v.41, no.4, 2020 , pp. 52-63 More about this Journal
Abstract
Objectives: In this study, we investigated the effects of Pueraria lobata Ohwi flower extracts (PLFE) on macrophages and their underlying mechanism(s) of action. PLFE increased the production of NO and cytokines (IL-6 and TNF-𝛼) in a dose-dependent manner, indicating its immunostimulatory property. Furthermore, PLFE upregulated iNOS, COX-2, and mitogen-activated protein kinase (MAPK) signaling in RAW264.7 cells. Additionally, PLFE enhanced the phosphorylation of I𝜅B𝛼 and subsequent I𝜅B𝛼 degradation, thereby enabling the nuclear translocation of NF-𝜅B. Taken together, these findings demonstrate that the immunostimulatory effects of PLFE are mediated by the nuclear translocation of the p65 subunit of NF-𝜅B and subsequent secretion of cytokines (IL-6 and TNF-𝛼), upregulation of iNOS and COX-2, and stimulation of MAPK signaling (JNK, ERK, and p38). Thus, PLFE may be a potential immunostimulatory therapeutic.
Keywords
Pueraria lobata Ohwi flower; PLFE; immunosimulatory effect;
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