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http://dx.doi.org/10.13048/jkm.13002

Lonicerae Flos Inhibits Cigarette-induced Lung Inflammatory Responses in Animal Model of Chronic Obstructive Pulmonary Disease  

Jung, Kyung-Hwa (Department of Physiology, College of Korean Medicine, Kyung Hee University)
Lee, Kye Seok (Department of Physiology, College of Korean Medicine, Kyung Hee University)
Kim, Youngeun (Department of Physiology, College of Korean Medicine, Kyung Hee University)
Park, Soojin (Department of Physiology, College of Korean Medicine, Kyung Hee University)
Hong, Moochang (Department of Physiology, College of Korean Medicine, Kyung Hee University)
Shin, Minkyu (Department of Physiology, College of Korean Medicine, Kyung Hee University)
Bae, Hyunsu (Department of Physiology, College of Korean Medicine, Kyung Hee University)
Publication Information
The Journal of Korean Medicine / v.34, no.2, 2013 , pp. 10-19 More about this Journal
Abstract
Objectives: In the present study, we evaluate the anti-inflammatory effect of Lonicerae Flos on cigarette-induced lung inflammatory responses in animal model of chronic obstructive pulmonary diseases (COPD). Methods: To inspect the effects of Lonicerae Flos, we evaluated Lonicerae Flos functions in vivo including immune cell profiles in bronchoalveolar lavage (BAL) fluid, cytokine production and tissue morphological changes. Results: Lonicerae Flos significantly inhibited immune cell infiltrations into the BAL fluid (neutrophils, macrophages, lymphocytes). TNF-${\alpha}$, and interleukin-6 (IL-6) were substantially decreased in the BAL fluid of Lonicerae Flos-treated mice compared with cigarette-exposed control mice. In addition, the hypertrophy of goblet cells in the epithelial cells was reduced in both Lonicerae Flos- and roflumilast-treated mice. Conclusions: The results of this study provide evidence that treatment with Lonicerae Flos exerts strong therapeutic effects against cigarette-induced lung inflammation in vivo. Therefore, this herbal medicine may represent a novel therapeutic agent for lung inflammation in general, as well as a specific agent for the treatment of COPD.
Keywords
COPD; cigarette; Lonicerae Flos; neutrophil; IL-6; TNF-${\alpha}$;
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1 Halbert RJ, Natoli JL, Gano A, Badamgarav E, Buist AS, Mannino DM. Global burden of COPD: systematic review and meta-analysis. Eur Respir J. 2006;28(3):523-32.   DOI   ScienceOn
2 Barnes PJ. Immunology of asthma and chronic obstructive pulmonary disease. Nat Rev Immunol. 2008;8(3):183-92.   DOI   ScienceOn
3 Fabbri L, Pauwels RA, Hurd SS. Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Pulmonary Disease: GOLD Executive Summary updated 2003. COPD. 2004;1(1):105-41; discussion 3-4.   DOI   ScienceOn
4 Rabe KF, Hurd S, Anzueto A, Barnes PJ, Buist SA, Calverley P, et al. Global strategy for the diagnosis, management, and prevention of chronic obstructive pulmonary disease: GOLD executive summary. Am J Respir Crit Care Med. 2007;176(6):532-55.   DOI   ScienceOn
5 Pesci A, Balbi B, Majori M, Cacciani G, Bertacco S, Alciato P, et al. Inflammatory cells and mediators in bronchial lavage of patients with chronic obstructive pulmonary disease. Eur Respir J. 1998;12(2):380-6.   DOI   ScienceOn
6 Vlahos R, Bozinovski S, Chan SP, Ivanov S, Linden A, Hamilton JA, et al. Neutralizing gran ulocyte/macrophage colony-stimulating factor in hibits cigarette smoke-induced lung inflammation. Am J Respir Crit Care Med. 2010;182(1):34-4   DOI   ScienceOn
7 Betsuyaku T, Hamamura I, Hata J, Takahashi H, Mitsuhashi H, Adair-Kirk TL, et al. Bronchiolar chemokine expression is different after single versus repeated cigarette smoke exposure. Respir Res. 2008;9:7.   DOI   ScienceOn
8 Boswell-Smith V, Spina D. PDE4 inhibitors as potential therapeutic agents in the treatment of COPD-focus on roflumilast. Int J Chron Obstruct Pulmon Dis. 2007;2(2):121-9.
9 Rabe KF, Tenor H, Dent G, Schudt C, Liebig S, Magnussen H. Phosphodiesterase isozymes modulating inherent tone in human airways: identification and characterization. Am J Physiol. 1993;264(5 Pt 1):L458-64.
10 Hatzelmann A, Schudt C. Anti-inflammatory and immunomodulatory potential of the novel PDE4 inhibitor roflumilast in vitro. J Pharmacol Exp Ther. 2001;297(1):267-79.
11 Kumar RK, Herbert C, Thomas PS, Wollin L, Beume R, Yang M, et al. Inhibition of inflammation and remodeling by roflumilast and dexamethasone in murine chronic asthma. J Pharmacol Exp Ther. 2003;307(1):349-55.   DOI   ScienceOn
12 Raouraoua-Boukari R, Sami-Merah S, Hammoudi-Triki D, Martin-Eauclaire MF, Laraba-Djebari F. Immunomodulation of the inflammatory response induced by Androctonus australis hector neurotoxins: biomarker interactions. Neuroimmunomodulation. 2012;19(2):103-10.   DOI   ScienceOn
13 George J, Ioannides-Demos LL, Santamaria NM, Kong DC, Stewart K. Use of complementary and alternative medicines by patients with chronic obstructive pulmonary disease. Med J Aust. 2004;181(5):248-51.
14 Lee H, Lee D, Kim Y, Lee G, Kim S-J, Jung S, et al. Lipopolysaccharide-induced lung inflammation is inhibited by Lonicera japonica Mol Cell Toxicol. 2011;7(1):87-93   DOI
15 Park E, Kum S, Wang C, Park SY, Kim BS, Schuller-Levis G. Anti-inflammatory activity of herbal medicines: inhibition of nitric oxide production and tumor necrosis factor-alpha secretion in an activated macrophage-like cell line. Am J Chin Med. 2005;33(3):415-24.   DOI   ScienceOn
16 Kim D, You B, Lim H, Lee SJ. Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury. Mol Pain. 2011;7:74.   DOI   ScienceOn
17 Xagorari A, Papapetropoulos A, Mauromatis A, Economou M, Fotsis T, Roussos C. Luteolin inhibits an endotoxin-stimulated phosphorylation cascade and proinflammatory cytokine production in macrophages. J Pharmacol Exp Ther. 2001;296(1):181-7.
18 Choi JM, Ahn MH, Chae WJ, Jung YG, Park JC, Song HM, et al. Intranasal delivery of the cytoplasmic domain of CTLA-4 using a novel protein transduction domain prevents allergic inflammation. Nat Med. 2006;12(5):574-9.   DOI   ScienceOn
19 Mannino DM, Buist AS. Global burden of COPD: risk factors, prevalence, and future trends. Lancet. 2007;370(9589):765-73.   DOI   ScienceOn
20 Stampfli MR, Anderson GP. How cigarette smoke skews immune responses to promote infection, lung disease and cancer. Nat Rev Immunol. 2009;9(5):377-84.   DOI   ScienceOn
21 Cosio MG, Saetta M, Agusti A. Immunologic aspects of chronic obstructive pulmonary disease. N Engl J Med. 2009;360(23):2445-54.   DOI   ScienceOn
22 Hogg JC, Timens W. The pathology of chronic obstructive pulmonary disease. Annu Rev Pathol. 2009;4:435-59.   DOI   ScienceOn
23 Hautamaki RD, Kobayashi DK, Senior RM, Shapiro SD. Requirement for macrophage elastase for cigarette smoke-induced emphysema in mice. Science. 1997;277(5334):2002-4.   DOI   ScienceOn
24 Siafakas NM, Vermeire P, Pride NB, Paoletti P, Gibson J, Howard P, et al. Optimal assessment and management of chronic obstructive pulmon ary disease (COPD). The European Respiratory Society Task Force. Eur Respir J. 1995;8(8):1398-420.   DOI   ScienceOn
25 Page CP, Spina D. Phosphodiesterase inhibitors in the treatment of inflammatory diseases. Handb Exp Pharmacol. 2011(204):391-414.
26 Clark CE, Arnold E, Lasserson TJ, Wu T. Herbal interventions for chronic asthma in adults and children: a systematic review and meta-analysis. Prim Care Respir J. 2010;19(4):307-14.   DOI   ScienceOn
27 Calverley PM, Rabe KF, Goehring UM, Kristiansen S, Fabbri LM, Martinez FJ. Roflumilast in symptomatic chronic obstructive pulmonary disease: two randomised clinical trials. Lancet. 2009;374(9691):685-94.   DOI   ScienceOn
28 Rabe KF, Bateman ED, O'Donnell D, Witte S, Bredenbroker D, Bethke TD. Roflumilast--an oral anti-inflammatory treatment for chronic obstructive pulmonary disease: a randomised controlled trial. Lancet. 2005;366(9485):563-71.   DOI   ScienceOn
29 Blanc PD, Trupin L, Earnest G, Katz PP, Yelin EH, Eisner MD. Alternative therapies among adults with a reported diagnosis of asthma or rhinosinusitis: data from a population-based survey. Chest. 2001;120(5):1461-7.   DOI   ScienceOn
30 Puljic R, Benediktus E, Plater-Zyberk C, Baeue rle PA, Szelenyi S, Brune K, et al. Lipopolysacc haride-induced lung inflammation is inhibited by neutralization of GM-CSF. Eur J Pharmacol. 200 7;557(2-3):230-5.   DOI   ScienceOn
31 Chung KF. Cytokines in chronic obstructive pulmonary disease. Eur Respir J Suppl. 2001;34:50s-9s.
32 Mukhopadhyay S, Hoidal JR, Mukherjee TK. Ro le of TNFalpha in pulmonary pathophysiology. Respir Res. 2006;7:125.   DOI   ScienceOn
33 Chen CY, Peng WH, Tsai KD, Hsu SL. Luteolin suppresses inflammation-associated gene expression by blocking NF-kappaB and AP-1 activation pathway in mouse alveolar macrophages. Life Sci. 2007;81(23-24):1602-14.   DOI   ScienceOn