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Effects of Hwangnyeonhaedok-tang on Cerebral Hemorrhage and Edema in Collagenase Induced-ICH Rats  

Ku, Ja-Seung (Div. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Lee, Joon-Suk (Div. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Shin, Jung-Won (Div. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Kim, Seong-Joon (Div. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Sohn, Nak-Won (Div. of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung Hee University)
Publication Information
The Journal of Korean Medicine / v.32, no.5, 2011 , pp. 100-113 More about this Journal
Abstract
Objectives: Brain edema is brain swelling that occurs due to the accumulation of excess water in the brain parenchyma. AQP4 and AQP9 are water-channel proteins expressed strongly in the brain and control water fluxes into and out of the brain parenchyma. This study was conducted to evaluate effect of Hwangnyeonhaedok-tang on brain edema and intracerebral hemorrhage. Methods: Intracerebral hemorrhage was induced by intrastriatal injection of type IV collagenase(0.23 U/${\mu}l$, 0.1 ${\mu}l$/min) into Sprague-Dawley rat brains. Hwangnyeonhaedok-tang water extract(1,000 mg/kg) was administered orally three times every 20 hours from 4 hours after ICH operation. Then hematoma volume, brain edema percentage, and water content of brain tissue were measured. Immuno-histochemistry was performed for AQP4 and AQP9 expressions in the brain sections and area % of immuno-labeling was analyzed with image analyzing system. Results: 1. Water extract of Hwangnyeonhaedok-tang reduced hematoma volume of ICH induced rat. 2. Water extract of Hwangnyeonhaedok-tang reduced MPO positive neutrophils in the perihematoma of the ICH induced rat. 3. Water extract of Hwangnyeonhaedok-tang reduced brain edema percentage and water content of brain tissue of ICH induced rat. 4. Water extract of Hwangnyeonhaedok-tang reduced AQP4 immuno-positive cells in the perihematoma of the ICH induced rat. 5. Water extract of Hwangnyeonhaedok-tang reduced AQP9 immuno-positive cells in the perihematoma of the ICH induced rat. Conclusions: These results suggest that Hwangnyeonhaedok-tang decreases intracerebral hemorrhage and brain edema by means of downregulating AQP4 and AQP9 expressions in the brain.
Keywords
Intracerebral hemorrhage; Hwangnyeonhaedok-tang; brain edema; MPO positive neutrophil; AQP4; AQP9;
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