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Anti-adipogenic Effect of Kaempferol, a Component of Polygonati Rhizoma  

Jang, Jae-Sik (Dept. of Internal Medicine, College of Korean Medicine, Dongguk University)
Jeong, Ji-Cheon (Dept. of Internal Medicine, College of Korean Medicine, Dongguk University)
Publication Information
The Journal of Korean Medicine / v.31, no.2, 2010 , pp. 158-166 More about this Journal
Abstract
Objective: It has been reported that Polygonati rhizoma (Pr) has anti-hyperglycemia, anti-triglycemia, anti-diabetic, and anti-tumor activity. Total extract of Pr was tested to identify anti-adipogenic activity in 3T3-L1 differentiation and molecular mechanism of Pr in 3T3-L1 differentiation. Methods: Differentiation of 3T3-L1 pre-adipocyte was induced in the presence of Pr extract and kaempferol. The level of lipid accumulation was measured by Oil Red O staining. The expression of genes associated with adipocyte differentiation was measured by RT-PCR. Results: Extract of Pr and its component kaempferol reduced lipid accumulation in 3T3-L1 during adipogenesis and also reduced mRNA levels of genes associated with adipogenesis, such as adipsin, aP2, LPL, SERBP-1c and $PPAR{\gamma}$. Conclusions: In this study, we showed that the molecular mechanism of Pr and kaempferol activity is related to regulation of $PPAR{\gamma}$ expression and activation.
Keywords
Polygonati rhizoma; kaempferol; adipogenesis; 3T3-L1 differentiation; $PPAR{\gamma}$; SREPB-1c;
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