Browse > Article
http://dx.doi.org/10.7837/kosomes.2016.22.5.500

Optimal Enrichment Temperature, Time and Materials for L-type Rotifer (Brachionus plicatilis) Cultured at a Low Temperature  

Yoo, Hae-Kyun (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
Byun, Soon-Gyu (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
Choi, Jin (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
Nam, Myeong-Mo (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
Moon Lee, Haeyoung (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
Kang, Hee Wong (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
Lee, Chu (Aquaculture Industry Research Division, East Sea Fisheries Research Institute)
Publication Information
Journal of the Korean Society of Marine Environment & Safety / v.22, no.5, 2016 , pp. 500-507 More about this Journal
Abstract
This study was undertaken to improve the survival and early life growth rates of cold-water fish by culturing rotifer (Brachionus plicatilis) with low-temperature tolerance. The enrichment experiment was carried out at different temperatures and over different time intervals. Cultivation of the rotifer at low temperatures was repeated, with the selected and cultured as the water temperature was gradually lowered from $20^{\circ}C$ to $10^{\circ}C$. Enrichment of the rotifer was completed using A, S, SCV and SCP. Enrichment was carried out after 6, 12 and 24 hours at three different temperatures (10, 15 and $20^{\circ}C$). In the growth experiments, the rotifer increased to approximately triple their original size, from $350{\pm}7.9ind./ml$ to $1,064{\pm}5.7ind./ml$ at $10^{\circ}C$ over 50 days. The fatty acid composition of the four enrichment materials was species-specific, with the highest ratios belonging to eicosapentaenoic acid (EPA, C20:5n-3) and docosahezaenoic acid (DHA, C22:6n-3) in SCP. The fatty acid composition of the rotifers was affected by the enrichment materials. The EPA (% of total fatty acid) was more than 2 % in SCP, which showed a higher ratio than the other enrichment materials. DHA was higher in S reaching 12.40 % at $15^{\circ}C$ for 24 hours. The highest levels of EPA (3.09 %) and DHA (11.65 %) were obtained after the rotifers were enriched with S at $20^{\circ}C$ for 12hours.
Keywords
Rotifer; Low temperature culture; Low temperature tolerance; Enrichment; n-3 HUFA;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Altaff, K. and A. Janakiraman(2015), Effect of temperature on mass culture of the three species of zooplankton, Brachionus plicatilis, Cerodaphnia treticulata and Apocyclops dengizicus. International Journal of Fisheries and Aquatic Studies, Vol. 2, No. 4, pp. 49-53.
2 Assavaaree, M., A. Hagiwara, K. Ide, K. Maruyama and E. Lubzens(2001), Low-temperature preservation (at $4^{\circ}C$) of marine rotifer Brachionus, Aquaculture Research, Vol. 32, No. 1, pp. 29-39.   DOI
3 Cahu, C. L., J. L. Z. Infante, A. Peres, P. Quazuguel and M. M. Le Gall(1998), Algal addition in sea bass (Dicentrarchus labrax) larvae rearing: effect on digestive enzymes, Aquaculture, Vol. 161, No. 1, pp. 479-489.   DOI
4 Epp, R. W. and P. W. Winston(1977), Osmotic regulation in the brackish-water rotifer Brachionus Plicatilis (Muller), Journal of Experimental Biology, Vol. 68, pp. 151-56.
5 Epp, R. W. and W. M. Lewis Jr(1980), Metabolic uniformity over the environmental temperature range in Brachionus plicatilis (Rotifera), Hydrobiologia, Vol. 73, pp. 145-147.   DOI
6 Fielder, D., G. Purser and S. Battaglene(2000), Effect of rapid changes in temperature and salinity on availability of the rotifers Brachionus rotundiformis and Brachionus plicatilis, Aquaculture, Vol. 189, pp. 85-99.   DOI
7 Folch, J., M. Lees and G. H. Sloane-Stanley(1957), A simple method for the isolation and purification of total lipids from animal tissues, Journal of Biological Chemistry, Vol. 226, No. 1, pp. 497-509.
8 Fu, Y., A. Hada, T. Yamashita, Y. Yoshida and A. Hino(1997), Development of a continuous culture system for stable mass production of the marine rotifer Brachionus, Hydrobiologia, Vol. 358, No. 1-3, pp. 145-151.   DOI
9 Furuita, H., T. Takeuchi and K. Uematsu(1998), Effects of eicosapentaenoic and docosahexaenoic acids on growth, survival and brain development of larval Japanese flounder (Paralichthys olivaceus), Aquaculture, Vol. 161, No. 1-4, pp. 269-279.   DOI
10 Furuita, H., T. Takeuchi, T. Watanabe, H. Fujimoto, S. Sekiya and K. Imaizumi(1996), Requirements of Larval Yellowtail for Eicosapentaenoic Acid, Docosahexaenoic Acid, and n-3 Highly Unsaturated Fatty Acid, Fisheries science, Vol. 62, No. 3, pp. 372-379.   DOI
11 Hagiwara, A., T. Kotani, T. W. Snell, M. Assava-Aree and K. Hirayama(1995), Morphology, reproduction, genetics, and mating behavior of small, tropical marine Brachionus strains (Rotifera), Journal of experimental marine biology and ecology, Vol. 194, No. 1, pp. 25-37.   DOI
12 Hirayama, K. and S. Ogawa(1972), Fundamental studies on physiology of rotifer for its mass culture. 1. Filter feeding of rotifer, Bulletin of the Japanese Society of Scientific Fisheries, Vol. 38, No. 11, p. 1207.   DOI
13 Miracle, M. R. and M. Serra(1989), Salinity and temperature influence in rotifer life history characteristics, Hydrobiologia, Vol. 186/187, pp. 81-102.   DOI
14 Ito, S., H. Sakamoto, M. Hori and K. Hirayama(1981), Morphological characteristics and suitable temperature for the growth of several strains of the rotifer, Brachionus plicatilis, Bulletin of the Faculty of Fisheries Nagasaki University, Vol. 51, pp. 9-16 (in Japanese with English abstract).
15 Koiso, M. and A. Hino(2001), Effects of salinity on population growth, nutritive value, and feeding cost of the enriched rotifer, Brachionus plicatilis, Suisanzoshoku, Vol. 49, No. 1, pp. 41-46 (in Japanese with English abstract).
16 Lubzens, E.(1987), Raising rotifers for use in aquaculture, Hydrobiologia, Vol. 147, No. 1, pp. 245-255.   DOI
17 Nakatani, T.(2008), Recent year class strengths of walleye pollock (Theragra chalcogramma) Pacific Population and oceanographic conditions for first-feeding pollock larvae, Bulletin of Fisheries Sciences Hokkaido University, Vol. 58, pp. 1-6 (in Japanese with English abstract).
18 NRC(1993), National Research Council, Nutrient requirements of fish National Academy Press, Washington, D.C, USA. pp. 1-124.
19 Oie, G. and Y. Olsen(1993), Influence of rapid changes in salinity and temperature on the mobility of the rotifer Brachionus plicatilis, Hydrobiologia, Vol. 255/256, pp. 81-86.   DOI
20 Onal, U, G. Topaloglu and A. Sepil(2015), The Performance of Continuous Rotifer (Brachionus Plicatilis) Culture System for Ornamental Fish Production. Journal of Life Sciences, Vol. 9, pp. 207-213.
21 Rainuzzo, J. R., Y. Olsen and G. Rosenlund(1989), The effect of enrichment diets on the fatty acid composition of the rotifer Brachionus plicatilis, Aquaculture, Vol. 79, No. 1-4, pp. 157-161.   DOI
22 Takeuchi, T.(2001), A review of feed development for early life stages of marine finfish in Japan. Aquaculture, Vol. 200, No. 1-2, pp. 203-222.   DOI
23 Rainuzzo, J. R., K. I. Reitan and Y. Olsen(1997), The significance of lipids at early stage of marine fish: a review, Aquaculture, Vol. 155, pp. 103-115.   DOI
24 Reitan, K. I., J. R. Rainuzzo, G. Oie and Y. Olsen(1997), A review of the nutritional effects of algae in marine fish larvae, Aquaculture, Vol. 155, pp. 207-221.   DOI
25 Seo, Y. S., M. E. Park, J. G. Kim and S. U. Lee(2007), Egg development and juvenile growth of the Pacific cod Gadus macrocephalus (Korean East Sea population), Korean Journal of Fisheries and Aquatic Sciences, Vol. 40, No. 6, pp. 380-386 (in Korean with english abstract).   DOI
26 Theilacker, G. H. and M. F. McMaster(1971), Mass culture of the rotifer Brachionus plicatilis and its evaluation as a food for larval anchovies, Marine Biology, Vol. 10, pp. 183-188.   DOI
27 Tomoda, T., M. Koiso and Y. Shima(2007), Dietary value of marine rotifer Brachionus plicatilis after enrichment produced by batch culture and extensive continuous culture methods, Nippon Suisan Gakkaishi, Vol. 73, No. 3, pp. 505-507 (in Japanese).   DOI
28 Walker, K. F.(1981), A synopsis of ecological information on the saline lake rotifer Brachionus plicatilis Muller 1786, Hydrobiologia, Vol. 81, No. 1, pp. 159-167.   DOI
29 Watanabe, T.(1993), Importance of docosahexaenoic acid in marine larval fish, Journal of the World Aquaculture Society, Vol. 24, No. 2, pp. 152-161.   DOI
30 Yoshimatsu, T., H. Imoto, M. Hayashi, K. Toda and K. Yoshimura(1997), Preliminary results in improving essential fatty acids enrichment of rotifer cultured in high density, Hydrobiologia, Vol. 358, No. 1-3, pp. 153-157.   DOI