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http://dx.doi.org/10.4217/OPR.2022015

Differential Expression of Three Novel Carbonic Anhydrases (CAs) Genes in Marine Dinoflagellate Prorocentrum minimum Against Various pH Conditions  

Shin, Jeongmin (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University)
Lee, Ha-Eun (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University)
Kim, Han-Sol (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University)
Ki, Jang-Seu (Department of Biotechnology, College of Biochemical Engineering, Sangmyung University)
Publication Information
Ocean and Polar Research / v.44, no.3, 2022 , pp. 209-220 More about this Journal
Abstract
Carbonic anhydrase (CA) is a key controller of the carbon concentrating mechanism (CCM), and is known to be affected by ambient pH and CO2 compositions. Herein, we characterized three novel CAs genes (PmCA1, 2, and 3) from the marine dinoflagellate Prorocentrum minimum, and evaluated the relative expressions of the PmCAs and photosynthetic genes PmatpB and PmrbcL under different pH conditions. Each PmCA was predicted to have amino acid residues constituting the zinc binding site. With signal peptide, PmCA1 and PmCA2 were predicted to be intracellular CAs located in the cytoplasm and chloroplast membrane, respectively. On the other hand, PmCA3 was predicted to be extracellular CA located in the plasma membrane. Also, PmCA1 was classified into the beta family, and PmCA2 and PmCA3 were classified into the alpha family via phylogenic analysis. The photosynthesis efficiency of P. minimum was similar at pH 7 to 9, and decreased significantly at pH 6 and pH 10. Overall, relative gene expression levels of the three PmCAs decreased at low pH, and increased as pH increased. Photosynthesis related genes, PmatpB and PmrbcL, showed similar expression patterns to those of PmCAs. These results suggest that changes in seawater pH may affect photosynthesis and CO2 metabolism in marine dinoflagellates.
Keywords
Prorocentrum minimum; Carbonic Anhydrase (CA); pH; ocean acidification; Carbon Concentrating Mechanism (CCM);
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