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http://dx.doi.org/10.4217/OPR.2013.35.3.229

Two-dimensional Oxygen Distribution in a Surface Sediment Layer Measured Using an RGB Color Ratiometric Oxygen Planar Optode  

Lee, Jae Seong (Oceanographic Measurement & Instrument Calibration Service Center, KIOST)
Kim, Eun-Soo (Ocean Observation Technology & Information Department, KIOST)
An, Sung-Uk (Oceanographic Measurement & Instrument Calibration Service Center, KIOST)
Kim, Jihye (Oceanographic Measurement & Instrument Calibration Service Center, KIOST)
Kim, Joung-Keun (Marine Environments & Conservation Research Division, KIOST)
Khang, Sung-Hyun (Oceanographic Measurement & Instrument Calibration Service Center, KIOST)
Kang, Dong-Jin (Oceanographic Measurement & Instrument Calibration Service Center, KIOST)
Publication Information
Ocean and Polar Research / v.35, no.3, 2013 , pp. 229-237 More about this Journal
Abstract
We measured two-dimensional (2-D) oxygen distribution in the surface sediment layer of intertidal sediment using a simple and inexpensive planar oxygen optode, which is based on a color ratiometric image approach. The recorded emission intensity of red color luminophore light significantly changed with oxygen concentration by $O_2$ quenching of platinum(II)octaethylporphyrin (PtOEP). The ratios between the intensity of red and green emissions with oxygen concentration variation demonstrated the Stern-Volmer relationship. The 2-D oxygen distribution image showed microtopographic structure, diffusivity boundary layer and burrow in surface sediment layer. The oxygen penetration depth (OPD) was about 2 mm and the one-dimensional vertical diffusive oxygen uptake (DOU) was 12.6 mmol $m^{-2}d^{-1}$ in the undisturbed surface sediment layer. However, those were enhanced near burrow by benthic fauna, and the OPD was two times deeper and DOU was increased by 34%. The simple and inexpensive oxygen planar optode has great application potential in the study of oxygen dynamics with high spatiotemporal resolution, in benthic boundary layers.
Keywords
oxygen planar optode; RGB color ratiometric; luminophore; $O_2$ quenching;
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