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http://dx.doi.org/10.22246/jikm.2022.43.6.1089

Study of the Effect and Underlying Mechanism of Clove Extract on Monosodium Iodoacetate-Induced Osteoarthritis in Rats  

Jin A Lee (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
Min Ju Kim (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
Seong-Wook Seo (Dept. of Rehabilitation Sciences, Graduate School, Daegu University)
Mi-Rae Shin (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Journal of Internal Korean Medicine / v.43, no.6, 2022 , pp. 1089-1104 More about this Journal
Abstract
Objective: The aim of this study was to identify the efficacy and underlying mechanism of cloves as an osteoarthritis (OA) treatment in a monosodium iodoacetate (MIA)-induced rat OA model. Osteoarthritis (OA) is nowadays one of the most prevalent degenerative joint diseases. Methods: Sprague-Dawley rats treated with MIA (50 μL; 80 mg/mL) were used as in vivo OA models. Cloves (100 and 200 mg/kg b.w.) were administered orally once daily for 2 weeks from 7 days after MIA injection. Changes in hindpaw weight distribution (HWD) were measured as a joint discomfort index. Activation markers related to inflammatory responses and cartilage degeneration in the right knee joints were evaluated by serum analysis and western blotting. Results: HWD decreased in the MIA control group but showed a dose-dependent elevation after clove treatment. Clove treatment inhibited inflammatory factors by PI3K/Akt/NF-κB signaling pathways, while also activating antioxidant factors through Sirt1/AMPK signaling pathways. Clove treatment also suppressed matrix metalloproteinase (MMP) overexpression and significantly increased the levels of tissue inhibitors of metalloproteinases (TIMPs). Conclusions: Treatment with cloves effectively reversed MIA-induced effects. Therefore, clove treatment could have the potential to protect against or treat OA.
Keywords
anti-inflammatory; cloves; MIA; osteoarthritis;
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