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http://dx.doi.org/10.22246/jikm.2022.43.4.514

The Effect of Trichosanthes Kirilowii Maximowicz Extract and Trichosanthes Kirilowii Maximowicz Cheonghyeol Plus on Anti-Inflammatory Factor Expression in Human Umbilical Vein Endothelial Cells (HUVECs)  

Kim, Hae-yoong (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Daejeon University)
Seol, In-chan (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Daejeon University)
Yoo, Ho-ryong (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Daejeon University)
Kim, Yoon-sik (Dept. of Circulatory Internal Medicine, College of Korean Medicine, Daejeon University)
Publication Information
The Journal of Internal Korean Medicine / v.43, no.4, 2022 , pp. 514-528 More about this Journal
Abstract
Objective: To examine the effects of Trichosanthes kirilowii Maximowicz extract (TE) and Trichosanthes kirilowii Maxi mowicz Cheonghyeol Plus Phellinus linteus Cheonghyeol plus (TCP) on anti-inflammatory factor expression in human umbilical vein endothelial cells (HUVECs). Methods: HUVECs were activated with TNF-α and then treated with TE and TCP. The expression levels were then measured for intracellular genes (KLF2, eNOS, MCP-1, ICAM-1, and VCAM-1), proteins (KLF2, eNOS, MCP-1, ICAM-1, VCAM-1, ERK, and JNK, p38), and extracellular biomarkers (ICAM-1, VCAM-1, and MCP-1). Results: 1. TCP at concentrations of 100 ㎍/mL or greater significantly increased the expression of KLF2 and eNOS intracellular genes and significantly decreased the expression of ICAM-1, VCAM-1, and MCP-1 genes compared to the control group. 2. TCP at concentrations of 100 ㎍/mL or greater significantly increased the expression of KLF2, eNOS proteins compared to the control group, and significantly reduced the expression of VCAM-1, ICAM-1, MCP-1, ERK, and p38 proteins. However, JNK protein phosphorylation showed no significant change compared to the control group. 3. TCP at concentrations of 100 ㎍/mL or more significantly decreased the production of MCP-1, ICAM-1, and VCAM-1 extracellular biomarkers compared to the control group. 4. TE at a concentration of 100 ㎍/mL did not cause any significant change in the expression of intracellular genes or proteins, in the production of the extracellular biomarker MCP-1, or in the amount of JNK protein compared to the control group. Other intracellular genes, proteins, and extracellular biomarker expression showed the same trend as observed with TCP exposure. Conclusion: This study experimentally confirmed that TE and TCP could be effective in preventing or inhibiting various inflammatory vascular diseases due to their anti-inflammatory effects.
Keywords
Trichosanthes kirilowii Maximowicz; ChungHuyl-Plus; atherosclerosis; herb medicine; anti-inflammatory effect;
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Times Cited By KSCI : 6  (Citation Analysis)
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