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http://dx.doi.org/10.22246/jikm.2021.42.1.1

The Effect of Chaenomelis Fructus Extract on Acute Hepatic Injury in Rats  

Lee, Jin A (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-Rae (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
Lee, Ji Hye (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
Roh, Seong-Soo (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
The Journal of Internal Korean Medicine / v.42, no.1, 2021 , pp. 1-10 More about this Journal
Abstract
Objective: This study was conducted to investigate the effect of Chaenomelis Fructus (CF) water extract on thioacetamide (TAA)-treated rats. Methods: Rats were divided into five groups: one normal group (n=8) and four with TAA-induced hepatic injury. These treatment groups were administered distilled water (n=8); silymarin 100 mg/kg (n=8); CF 100 mg/kg (n=8); and CF 200 mg/kg (n=8). In the TAA groups, the acute liver injury was induced via IP injection (200 mg/kg), and the silymarin and CF extract were then orally administered for three days. Subsequently, serum levels of GOT, GPT, and ammonia were confirmed as well as protein expressions using liver tissue. Results: In the liver injury-induced rats, CF administration reduced tissue damage and serum levels of GOT, GPT, and ammonia. In addition, CF increased the anti-oxidant proteins Nrf2, Keap1, HO-1, and catalase and significantly regulated matrix metalloproteinases (MMP-2 and MMP-9) and their tissue inhibitors (TIMP-1 and TIMP-2). Conclusions: In this animal model of liver injury induced by TAA, CF extract is determined to have a hepatoprotective effect by increasing anti-oxidant proteins that relieve damage and by regulating the expression of matrix metalloproteinases.
Keywords
Chaenomelis Fructus; acute hepatic injury; thioacetamide; anti-oxidant;
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1 全國韓醫科大學 肝系內科學 교수 공저. 肝系內科學. 서울: 東洋醫科學硏究院; 1992, p. 164-76.
2 Campos R, Garrido A, Guerra R, Valenzuela A. Silybin dihemisuccinate protects against glutathione depletion and lipid peroxidation indeced by acetaminophen on rat liver. Planta Medica 1989;55(5):417-9.   DOI
3 Junqueira VB, Simizu K, Videla LA, Barros SB. Dose-dependent study of the effects of acute lindane administration on rat liver superoxide anion production, antioxidant enzyme activities and lipid peroxidation. Toxicology 1986;41(2):193-204.   DOI
4 Moody CS, Hassan HM. Mutagenicity of oxygen free radicals. Proc Natl Acad Sci USA 1982;79(9):2855-9.   DOI
5 Murakami T, Kim T, Nakamura H. Hepatitis, cirrhosis, and hepatoma. J Magn Reson Imaging 1998;8(2):346-58.   DOI
6 Reichen J. Assessment of hepatic function with xenobiotics. Semin Liver Dis 1995;15(3):189-201.   DOI
7 Aratani Y. Myeloperoxidase: Its role for host defense, inflammation, and neutrophil function. Arch Biochem Biophys 2018;640:47-52   DOI
8 Ryu HW, Kim YS, Lim EM. The Antiinflammatory Effects of Chaenomelis Fructus Herba Water Extract on Mouse RAW 264.7 Cell. J Korean Obstet Gynecol 2012;25(3):1-15.   DOI
9 Kim JE, Jo YJ, Leem JY. The Acetylcholinesterase Inhibitory Activity of the EtOH Extract of Chaenomelis Fructus and its effects on the Metabolism of Amyloid Precursor Protein in Neuroblastoma Cells. Kor J Pharmacogn 2015;46(4):327-33.
10 Kang SY, Hyun SY, Kwon YD, Park YK, Jung HW. Effects of Chaenomelis Fructus Extract on the regulation of myoblasts differentiation and the expression of biogenetic factors in C2C12 myotubes. Kor J Herbol 2019;34(6):99-107.
11 Kim YM, Hwang DS, Kwak BM, Kim EH. Protective Effect of Herbal Mixture Including Lycii Fructus on Hepatotoxicity Induced by Thioacetamide in Mice. Korean J Acupuncture 2019;36(4):221-9.   DOI
12 Kim JB, Sohn JH, Lee HL, Kim JP, Han DS, Hahm JS, et al. Clinical Characcteristics of Acute Toxic Liver Injury. The Korean J Hepatol 2004;10(2):125-34.
13 Yoo JH, Han SH, Kil GJ. Active Effect of Anticoagulant Effects in Chaenomelis Fructus Water Extract. Kor J Herbol 2009;24(2):7-11.
14 Lee YM, Shin HD, Lee JJ, Lee MY. Antioxidative Effect of Chaenomelis Fructus Ethanol Extract. Korean J Food Preserv 2007;14(2):177-82.
15 Kim DH, Kwon BR, Kim SJ, Kim HJ, Jeong SI, Yu KY, Kim SY. Anti-obese Effects and Signaling Mechanisms of Chaenomeles sinensis extracts in 3T3-L1 Preadipocytes and Obese Mice Fed a High-fat Diet. Herbal Formula Science 2017;25(4):457-69.   DOI
16 Lee YM, Lee JJ, Shim HD, Lee MY. Protective Effects of Chaenomeles sinensis Koehne Extract on Ethanol-induced Liver Damage in Rat. J Korean Soc Food Sci Nutr 2006;35(10):1336-42.   DOI
17 Germoush MO, Othman SI, Al-Qaraawi MA, Al-Harbi HM, Hussein OE, Al-Basher G, et al. Umbelliferone prevents oxidative stress, inflammation and hematological alterations, and modulates glutamate-nitric oxide-cGMP signaling in hyperammonemic rats. Biomed Pharmacother 2018;102:392-402.   DOI
18 Lee DH, Kim JH, Kim NM, Choi JS, Lee JS. Physiological functionality of chinese quince wine and liqors. Korean J Biiotechnol Bioeng 2002;17(3):266-70.
19 Fitzhugh OG, Nelson AA. Liver Tumors in Rats Fed Thiourea or Thioacetamide. Science 1948;108(2814):626-8.   DOI
20 Kim SY, Kim HP, Lee MK, Byun SJ, Kim SH, Han HM, et al. The effect of betaine on the CC14-induced hepatoxicity in rats. Yakhak hoeji 1993;37(5):538-43.
21 Kim KJ, Shin MR, Kim SH, Kim SJ, Lee AR, Kwon OJ, et al. Protective effect of Tongyuhwalhyeol-tang on liver injury in thioacetamide-induced rat. Kor J Herbol 2018;33(1):37-46.   DOI
22 He HJ, Wang GY, Gao Y, Ling WH, Yu ZW, Jin TR. Curcumin attenuates Nrf2 signaling defect, oxidative stress in muscle and glucose intolerance in high fat diet-fed mice. World J Diabetes 2012;3(5):94-104.   DOI
23 Lee MS, Lee J, Kwon DY, Kim MS. Ondamtanggamibang protects neurons from oxidative stress with induction of heme oxygenase-1. J Ethnopharmacol 2016;108(2):294-8.   DOI
24 Kim SE, Jung SA, Shim KN, Jung HK, Kim TH, Yoo K. Expression of Matrix Metallopreoteinases and Tissue Inhibitors of Metalloproteinases in Ulcerative Colitis. Intest Res 2009;7(1):32-40.