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http://dx.doi.org/10.22246/jikm.2020.41.6.967

The Effect of Acanthopanax sessiliflorum Cheonghyeol Plus on NF-κB and MAPKs Signaling and Vascular Attachment Factors  

Lee, Ji-won (Dept. of Korean Internal Medicine, Dae-Jeon Jaseng Hospital of Korean Medicine)
Choi, Gyu-cheol (Dept. of Korean Internal Medicine, Dae-Jeon Jaseng Hospital of Korean Medicine)
Bae, Ji-eun (Dept. of Korean Internal Medicine, Dae-Jeon Jaseng Hospital of Korean Medicine)
Yoo, Ho-ryong (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-Jeon University)
Kim, Yoon-sik (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-Jeon University)
Seol, In-chan (Dept. of Cardiology and Neurology of Korean Medicine, College of Korean Medicine, Dae-Jeon University)
Publication Information
The Journal of Internal Korean Medicine / v.41, no.6, 2020 , pp. 967-983 More about this Journal
Abstract
Objective: This study was performed to investigate the effect of Acanthopanax sessiliflorum Cheonghyeol plus (ASCP) on NF-κB and MAPK signaling and vascular adhesion factors associated with dyslipidemia in human umbilical vein endothelial cells (HUVECs). Methods: We measured the scavenging activity of DPPH radical and ABTS radical by ASCP in HUVECs. We measured the protein expression levels of NF-κB, IκBα, ERK, JNK, and p38 after treatment of HUVECs with TNF-α. We measured the expression levels of MCP-1, ICAM-1, and VCAM-1 mRNA and of MCP-1, ICAM-1, and VCAM-1 biomarkers after treatment of HUVECs with TNF-α. Results: The DPPH and ABTS radical scavenging activity of ASCP increased in a concentration-dependent manner. NF-κB, IκB, ERK, p38 protein expression levels decreased following ASCP treatment at all concentrations compared to untreated control HUVECs. JNK protein expression levels decreased in ASCP-treated HUVECs compared to untreated controls at concentrations of 100 ㎍/mL. MCP-1 mRNA expression level decreased with ASCP treatment ≥200 ㎍/mL compared to the control. ICAM-1 and VCAM-1 mRNA expression levels decreased at all concentrations compared to the control. MCP-1 protein expression level was reduced compared to the control at concentrations ≥200 ㎍/mL, ICAM-1 protein expression level was reduced compared to the control at concentrations ≥100 ㎍/mL, and VCAM-1 protein expression level was reduced at all concentrations. Conclusions: These results suggest that ASCP has an antioxidative and hypolipidemic effect and that ASCP could treat and prevent dyslipidemia, atherosclerosis, and cardio-cerebrovascular diseases.
Keywords
Acanthopanax sessiliflorum; Cheonghyeol-Plus; dyslipidemia; HUVECs; antioxidation activity;
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Times Cited By KSCI : 8  (Citation Analysis)
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