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http://dx.doi.org/10.22246/jikm.2019.40.4.567

Inhibitory Effects of SGX01 on Lung Injury of COPD Mice Model  

Park, Jae-jun (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Yang, Won-kyung (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Lyu, Yee Ran (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Kim, Seung-hyung (Institute of Traditional Medicine and Bioscience, Dae-Jeon University)
Park, Yang Chun (Division of Respiratory System, Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Publication Information
The Journal of Internal Korean Medicine / v.40, no.4, 2019 , pp. 567-581 More about this Journal
Abstract
Objective: This study aimed to evaluate the inhibitory effects of SGX01 on the lung injuries of COPD mice model. Materials and Methods: This study was carried out in two ways: in vitro and in vivo. In vitro, L929 cells were challenged with LPS, and then treated with six concentrations of SGX01 (10, 30, 50, 100, 300, and $500{\mu}g/ml$) and analyzed by ELISA. In vivo, C57BL/6 mice were challenged with LPS and cigarette smoking solution (CSS), and then treated with a vehicle only (control group), dexamethasone 3 mg/kg (dexa group), or a SGX01 200 mg/kg (SGX01 group). After sacrifice, the BALF or lung tissue was analyzed with Cytospin, FACS, ELISA, real-time PCR and H&E, and Masson's trichrome staining. Results: SGX01 significantly decreased NO, $TNF-{\alpha}$, and IL-6 on L929 cells challenged with LPS. In the COPD model, SGX01 significantly inhibited the increase of neutrophils, $TNF-{\alpha}$, IL-17A, CXCL-1, MIP2, CD8+ cells in BALF, and $TNF-{\alpha}$, $IL-1{\beta}$ mRNA expression in lung tissue. It also decreased the severity of the histological lung injury. Conclusion: This study suggests the usability of SGX01 for COPD patients by controlling lung tissue injury.
Keywords
SGX01; chronic obstructive pulmonary disease; cigarette smoke solution;
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