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http://dx.doi.org/10.22246/jikm.2019.40.3.331

Comparison between the Antioxidant Activity and the Index Content of ACTS002 according to the Extraction Solvent  

Lee, Dae-yeon (The Fore)
Sim, Sun-hyung (Forest Hospital)
Kim, Wan-su (Forest Hospital)
Yi, Young-woo (The Fore)
Lee, In-hee (The Fore)
Publication Information
The Journal of Internal Korean Medicine / v.40, no.3, 2019 , pp. 331-342 More about this Journal
Abstract
Objectives: Samul-tang is commonly used to alleviate the side effects of chemotherapy. This study aimed to establish an efficient method of extracting ACTS002 based on Samul-tang using the yield, high-performance liquid chromatography (HPLC), and antioxidant assay. Methods: ACTS002 was extracted from each extraction solvent, and the contents of 5-hydroxymethyl-2-furaldehyde (5-HMF), paeoniflorin, and ferulic acid were quantitatively analyzed and compared using HPLC. Moreover, the antioxidant activities of ACTS002 were measured using total flavonoids, total phenolic compounds, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and Ferric reducing/antioxidant power (FRAP). Results: All of the components were set as the index contents because they were easy to process. The antioxidant activity of total flavonoids was the highest in 70% ethyl alcohol extracts, and total phenolic compounds were the highest in 50% ethyl alcohol extracts. In DPPH, 50% ethyl alcohol extracts showed the highest activity, and in ABTS 70% ethyl alcohol extracts were the highest. In FRAP, 70% ethyl alcohol extracts showed the highest activity. Conclusions: ACTS002 can control quality by setting 5-HMF, paeoniflorin, and ferulic acid as the index contents. The antioxidant activity measurement was relatively high in the 50% and 70% ethyl alcohol extracts. Our results can predict the possibility of a pharmacological activity and the standardization of ACTS002.
Keywords
ACTS002; yield; HPLC; antioxidant;
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1 Kim SH. Clinical Comparison with Drug Sensitivities by the Human Tumor Clonogenic Assay. J Korean Cancer Assoc1989;21(1):11-8.
2 Park CG, Lim DK, Kook YH, Cha CR, Paik CG. In Vitro Chemosensitivity of Doxorubicin on Human Cancer Cell Lines. J Korean Cancer Assoc 1990;22(1):61-6.
3 Willson JKV, Bittner GN, Oberley TD, Meisner LF, Weese JL. Cell Culture of Human Colon Adenomas and Carcinomas. Cancer Res 1987; 47(10):2704-13.
4 Yang GH, Jeong HW, Choi JH. Effects of Naetakcheonkeumsan and It's Gamypang on the Lymphocytes and Cancer cells. The Journal of Oriental Medical Surgery, Ophthalmology & Otolaryngology 2000;13(1):44-59.
5 Kim JH, Lee JK, Seo CS, Ha HK, Lee HY, Jung DY, et al. Analysis of Studies on Samul-tang for Fundamental Establishment of Evidence Based Medicine. J Physiol & Pathol Korean Med 2009;23(4):779-88.
6 Cho BW, Lee KS, Song BK. Effects of A Constituent Herbs of Samul-tang on Antithrombosis and Antistress. J Oriental Gynecol 1999;12(2):41-74.
7 Kim SH, Oh H, Lee SE, Jo SK, Byun MW. Effect of Si-Wu-Tang and Si-Jun-Zi-Tang on the Survival of Jejunal Crypt Cells and Hematopoietic Cells in Irradiated Mice. Korean J Food Sci Technol 1998;30(4):888-94.
8 Lee YC, Jeon BH. Study on the Anticancer Effect of Samultang and Sagoonjatang with Semen Tiglii Extract on Human Cancer Cell Lines and Sarcoma 180. J of Oriental Med Pathol 1994;9(1):79-100.
9 Seo CS, Ha HK, Jung DY, Lee HY, Shin HK. Evaluation of the Immune-Stimulating Activity of Samul-tang, a Traditional Korean Herbal Medicine, Standardized by HPLC-PDA. The Journal of Korean Oriental Medicine 2011;32(3):25-34.
10 Kim NM, Sung HS, Kim WJ. Effect of Solvents and Some Extraction Conditions on Antioxidant Activity in Cinnamon Extracts. Korean J Food Sci Technol 1993;25(3):204-9.
11 Kim YH, Kim SH, Chang HJ, Park JK, Jeong MY, Park YS. Survey on the Preference Formula for the Improvement of Oriental Herbal Medicine Insurance. Kor J Herbology 2009;24(4):17-23.
12 Jin SE, Seo CS, Ha HK, Lim HS, Kim YJ, Jeon WY, et al. Comparative Study on Biological Activities and Ingredient Contents of Different Solvent Extracts of Samchulkunbi-tang. Kor J Pharmacogn 2012;43(4):328-37.
13 Choi MA, Kim ML, Park CS. The Antibacterial and Antioxidative Activities of Samultang Ingredient Extracts. Korean J Food Cookery SCI 2008;24(1):52-8.
14 Kim IR, Hwang KH, Joo HJ, Ma JY. The studies about cutting, processing and decokting methods of oriental medicinal plants II : Rehmanniae Radix Preparata. Korean J Ori Med 1998;4(1):115-27.
15 Lim EM, Jang JB, Lee KS, Song BK. Experimental studies on Samultang and herbal compositions in Samultang. Journal of KyungHee Oriental Medicine College 1998;21(1):409-32.
16 Park HR, Kim SH, Yee ST, Byun MW, Jo SK. Effect of a Herb Mixture (HIM-1) on the Protection of the Hematopoietic-Immune System and Self-renewal Tissues against Radiation Damage. J Korean Soc Food Sci Nutr 2005;4(5):605-12.
17 Azuma K, Nakayama M, Koshioka M, Ippoushi K, Yamaguchi Y, Kohata K, et al. Phenolic antioxidants from the leaves of Corchorus olitorius L. J Agric Food Chem 1999;47(10):3963-9.   DOI
18 Song DS, Woo KS, Seong NS, Kim KY, Jeong HS, Lee HB. Changes in Quality of Rehmanniae radix Preparata with Heating Conditions. J Korean Soc Food Sci Nutr 2007;36(6):773-8.   DOI
19 Lee CK, Seo JM. Changes of the Constituents in the Rehmanniae Radix Preparata during Processing. J Korean Soc Food Sci Nutr 2004;33(10):1748-52.   DOI
20 Kim NY, Jang HK, Yang KH, Lee KJ, Kim MR. Antioxidant Activities and Quality Characteristics of Jelly Added Rehmannia Radix Preparata Concentrate. J East Asian Soc Dietary Life 2011;21(6):814-22.
21 Park SG, Lee MJ, Jung HJ, Lee HS, Kim H, Na ST, et al. Preventive Effects of Peony Root Extracts on Oxidative Stress, Thrombosis and Atherosclerosis. J Korean Oriental Med 2009;30(2):88-103.
22 Tanaka T, Kataoka M, Tsuboi N, Kouno I. New Monoterpene Glycoside Esters and Phenolic Constituents of Paeoniae Radix, and Increase of Water Solubility of Proanthocyanidins in the Presence of Paeoniflorin. Chem Pharm Bull 2000;48(2):201-7.   DOI
23 Kang SS, Kim JS, Kim EM, Yun-Choi HS. Platelet Anti-Aggregation of Paeony Root. Kor J Pharmacogn 1991;22(4):215-8.
24 Kim YE, Lee YC, Kim HK, Kim CJ. Antioxidative Effect of Ethanol Fraction for Several Korean Medicinal Plant Hot Water Extracts. Korean J Food & Nutr 1997;10(2):141-4.
25 Ro HS, Ko WK, Yang HO, Park KK, Cho YH, Lee YE, et al. Isolation of Hyperlipidemic Substances from Methanol Extract of Paeonia Radix. J Kor Pharm Sci 1999;29(1):55-60.
26 Oh YJ, Seo HR, Choi YM, Jung DS. Evaluation of Antioxidant Activity of the Extracts from the Aerial parts of Cnidium officinale Makino. Korean J Medicinal Crop Sci 2010;18(6):373-8.
27 Kwak DH, Kim JK, Kim JY, Jeong HY, Keum KS, Han SH, et al. Anti-angiogenic activities of Cnidium officinale Makino and Tabanus bovinus. Journal of Ethnopharmacology 2002;81(3):373-9.   DOI
28 Kim JM, Son D, Lee P, Lee KJ, Kim H, Kim SY. Ethyl Acetate Soluble Fraction of Cnidium officinale MAKINO Inhibits Neuronal Cell Death by Reduction of Excessive Nitric Oxide Production in Lipopolysaccharide-Treated Rat Hippocampal Slice Cultures and Microglia Cells. J Pharmacol Sci 2003;92(1):74-8.   DOI
29 Lee JM, Chung H, Chang PS, Lee JH. Development of a method predicting the oxidative stability of edible oils using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Food Chemistry 2006;103(2):662-9.   DOI
30 Lee JH, Choi HS, Chung MS, Lee MS. Volatile Flavor Components and Free Radical Scavenging Activity of Cnidium officinale. Korean J Food Sci Technol 2002;34(2):330-8.
31 Choi HS, Lee Kim MS, Sawamura M. Constituents of the essential oil of cnidium officinale Makino, a Korean medicinal plant. Flavour Fragr J 2002;17(1):49-53.   DOI
32 Lee EJ. Control of Citrus Blue Mold and Chili Pepper Anthracnose by Ligustilide, an Antifungal Compound from Cnidium officinale Makino. MS Thesis, Seoul National University 2004.
33 Kim HS, Joung SW. Effective Components and Nitrite Scavenging Ability of Root and Leaves a Angelica gigas Nakai. Korean J Food Cookery Sci 2006;22(6):957-65.
34 Kim MS, Oh YJ. An Investigative Analysis of Preference and Uses for the Angelica gigas Nakai- Focused on the consumer in the Seoul and Kyunggido Area-. J East Asian Soc Dietary Life 2009;19(5):783-90.
35 Liu SP, Dong WG, Wu DF, Luo HS, Yu JP. Protective effect of angelica sinensis polysaccharide on experimental immunological colon injury in rats. World J Gastroenterol 2003;9(12):2786-90.   DOI
36 Lee DY, Choi BS, Lee IH, Kim JH, Gwon PS. Comparison of Index Compounds Content and Antioxidative Activity of Wild Ginseng Pharmacopuncture by Extraction Methods. J Int Korean Med 2018;39(3):313-22.   DOI
37 Chae SK, Kang GS, Ma SJ, Bang KW, Oh SH. Standard food analysis. Seoul: Jigu-moonwha Sa; 2002, p. 381-2.