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http://dx.doi.org/10.22246/jikm.2018.39.1.1

The Effect of Gamidohongsamul-tang (GDT) on the Gene Expression Levels of eNOS, KLF2, ICAM-1 and VCAM-1 in HUVEC Cells  

Lim, Hyun-chan (Dept. of Oriental Internal Medicine, Dong-Shin University Gwangju Oriental Hospital)
Jeon, Sang-yoon (Dept. of Oriental Internal Medicine, Dong-Shin University Gwangju Oriental Hospital)
Publication Information
The Journal of Internal Korean Medicine / v.39, no.1, 2018 , pp. 1-8 More about this Journal
Abstract
Objectives: This study seeks to investigate the effects of Gamidohongsamul-tang (GDT) on the Gene expression levels of eNOS, KLF2, ICAM-1 and VCAM-1 in HUVEC cells. Methods: HUVEC cells were treated at a concentration of 1, 10, 100 (${\mu}g/ml$) of Gamidohongsamul-tang (GDT). To measure the NOS, KLF2, ICAM-1 and VCAM-1 gene expression in HUVEC cells, the synthesized cDNA was subjected to polymerase chain reaction (PCR) and electrophoresis was performed to verify gene expression level. Results: 1. GDT significantly increased eNOS and KLF2 gene expression. 2. GDT significantly reduced ICAM-1 and VCAM-1 gene expression. Conclusions: These experiments suggest that Gamidohongsamul-tang (GDT) regulates gene expression related with anti-dyslipidemic effects in HUVEC cells. In order to clinically apply this to diseases related to dyslipidemia, such as cardiovascular disease, additional in vivo experiments are needed to verify the anti-dyslipidemic effects of GDT.
Keywords
Gamidohongsamul-tang; anti-dyslipidemic; HUVEC cell;
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Times Cited By KSCI : 2  (Citation Analysis)
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