Browse > Article
http://dx.doi.org/10.22246/jikm.2017.38.6.1021

Therapeutic Effects of Gaejigayonggolmoryo-tang on Dextran Sodium Sulfate-induced Ulcerative Colitis in Mice  

Kang, Amy (Dept. of Internal Medicine, College of Korean Medicine, Dong-Guk University)
Lim, Seong-woo (Dept. of Internal Medicine, College of Korean Medicine, Dong-Guk University)
Publication Information
The Journal of Internal Korean Medicine / v.38, no.6, 2017 , pp. 1021-1034 More about this Journal
Abstract
Objectives: The aim of this study was to investigate the effects of Gaejigayonggolmoryo-tang (GYT) on ulcerative colitis induced by dextran sodium sulfate (DSS) in mice. Methods: Colitis was induced by free drinking of 5% DSS in six-week-old male ICR mice. The experimental groups were the sample group, the control group, and the normal group. The sample group was treated with GYT for three days after being was given 5% DSS for five days. The control group was given water, instead of GYT, for three days after the five days of 5% DSS. The normal group was untreated (not given 5% DSS), for comparison purposes. Results: Cellular experiments showed that GYT inhibits the expression of the inflammatory enzymes COX-2 and iNOS, and the production of NO. Based on the primary cellular experiments, the effects of GYT on ulcerative colitis induced by DSS of mouse tissues were investigated. GYT reduced tissue damage and apoptosis by inhibiting the expression of the inflammatory enzymes $NF-{\kappa}B$ p65, COX-2, and iNOS. In the cellular experiment, GYT was more effective in inhibiting the expression of COX-2 than in inhibiting the expression of iNOS. GYT was evidently effective in tissues in inhibiting the expression of COX-2. Conclusions: Based on the results here, GYT may have therapeutic effects on ulcerative colitis induced by DSS. GYT is worthy of research and development as a COX-2 inhibitor and a potential drug for inflammatory bowel diseases from natural products. Further investigations for exact mechanisms will be needed.
Keywords
Gaejigayonggolmoryo-tang; ulcerative colitis; dextran sulfate sodium (DSS);
Citations & Related Records
Times Cited By KSCI : 10  (Citation Analysis)
연도 인용수 순위
1 Van der Sluis M, De Koning BA, De Bruijn AC, Velcich A, Meijerink JP, Van Goudoever JB, et al. Muc2-deficient mice spontaneously develop colitis, indicating that muc2 is critical for colonic protection. Gastroenterology 2006;131(1):117-29.   DOI
2 Rosillo MA, Sanchez-Hidlgo M, Cardeno A, Aparicio-Soto M, Sanchez-Fidalgo S, Villegas I, et al. Dietary supplementation of an ellagic acid-enriched pomegranate extract attenuates chronic colonic inflammation in rats. Pharmacological Research 2012;66(3):235-42.   DOI
3 Nathan C, Xie QW. Nitric oxide synthase: roles, tolls and controls. Cell 1994;78(6):915-8.   DOI
4 Mitchell JA, LarkimS, Williams TJ. Cyclooxygenase-2: regulation and relevance in inflammation. Biochem Pharmacol 1995;50(10):1535-42.   DOI
5 Wei J, Feng J. Signaling pathways associated with inflammatory bowel disease. Recent Pat Inflamm Allergy Drug Discov 2010;4(2):105-17.   DOI
6 Hengartmer MO. The biochemistry of apoptosis. Nature 2000;407(6805):770-6.   DOI
7 Martin AR, Villegas I, La Casa C, Alarcon de la Lastra C. The cyclo-oxygenase-2 inhibitor, rofecoxib, attenuates mucosal damage due to colitis induced by trinitrobenzene sulphonic acid in rats. European J Pharmacology 2003; 481(2-3):281-91.   DOI
8 Jung Sang Ji Ja, Pathophysis, Seoul: Gunja Publishing Co., Ltd.; 2015, p. 51.
9 Yang SK. Current Status and Clinical Characteristics of Inflammatory Bowel Disease in Korea. The Korean journal of gastroenterology 2002;40(1):1-14.
10 Yang SK, Hong WS, Min YI, Kim HY, Yoo JY, Rhee PL, et al. Incidence and prevalence of ulcerative colitis in the Songpa-Kangdong District, Seoul, Korea, 1986-1997. J Gastroenterol Hepatology 2000;15(9):1037-42.   DOI
11 Department of gastrointestinal medicine Korean medicine. gastrointestinal internal medicine. Seoul: Gunja Publishing Co., Ltd.; 2008, p. 365, 374.
12 Seo CH, Lee HH, Choi KS, Lim DS, Ryu HC, Lee YS, et al. Two Cases of Ulcerative Colitis Diagnosed as Damp-Heat Dysentery Treated with Jakyaktang-gamibang. The Journal of Korean Oriental Internal Medicine 2006;27(4):984-90.
13 Bae KH, Kon KH. Effects of Goihwa-san on Ulcerative Colitis Induced by Dextran Sulfate Sodium in Mice. The Journal of Korean Oriental Internal Medicine 2010;31(3):513-25.
14 Hwang JW, Choi HJ, Baik YS, Jeong SH, Shin GC, Lee WC. A Case of Pyungwijiyutang-gamibang Diagnosed as Constipation due to Stagnation of Eum with Ulcerative Colitis. The Journal of Korean Oriental Internal Medicine 2007;28(4):972-77.
15 Kim SY, Ryu BH, Park JW. Effects of Samiunkyungtang on inflammation and fecal enzymes in ulcerative colitis animal model. Journal of Korean Medicine 2008;29(3):50-62.
16 Lee SH, Choi HM, Lim SW. Effects of Jiyutang on DSS-induced Colitis of the Mouse. Journal of Korean Medicine 2007;28(1):187-97.
17 Jangjunggyeong. Kyeungakjunseo. Seoul: Daeseong Publishing Co., Ltd.; 1988, p. 370, 414.
18 Hwang EY, Kim DH, Kim HJ, Hwang JY, Park TS, Lee IS, et al. Antioxidant Activities and Nitric Oxide Production of Medicine Plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia Lactiflora). J Appl Biol Chem 2011;54(3):171-7.   DOI
19 Park HJ, Lee JS, Lee JD, Kim NJ, Pyo JH, Kang JM, et al. The Anti-inflammatory Effect of Cinnamomi Ramulus. Journal of Korean Medicine 2005;26(2):140-51.
20 He ZD, Qiao CF, Han QB, Cheng CL, Xu HX, Jiang RW, et al. Authentication and quantitative analysis on the chemical profile of cassia bark(cortex cinnamomi) by high-pressure liquid chromatography. J Agric Food Chem 2005; 53(7):24-8.
21 Kim HY, Han YM. Anti-inflammatory Effect of Paeoniflorigenone Isolated from Paeoniae Radix. Archives of Pharmacal Research 2012;56(1):20-5.
22 Kim YJ, Son DY. Inflammatory mediator regulation of the Zizyphus jujube leaf fractions in the LPS-stimulated Raw264.7 mouse machrophage. Korean J Food Preser 2014;21(1):114-20.   DOI
23 Korean Federation of Internal Medicine compilation Harrison's Internal Medicine. Seoul: Do Seo Publising MIP; 2006, p. 1937-50.
24 Kim SJ, Kim SH, Lim YI, Kim YG, Park KY. Inhibitory Effects of Ginger and Beopje Ginger on DSS-induced Colitis in Mice. J Korean Soc Food Sci Nutr 2014;43(4):477-84.   DOI
25 Lee KH, Rhee KH. Anti-Inflammatory Effects of Glycyrrhiza glabra Linne Extract in a Dextran Sulfate Sodium-Induced Colitis Mouse Model. Korean J Food & Nutr 2010;23(4):435-9.
26 Shibata S. A drug over the millennia: pharmacognosy, chemistry, and pharmacology of licorice. Yakugaku Zasshi 2000;120(10):849-62.   DOI
27 Jeong HG, You HJ, Park SJ, Moon AR, Chung YC, Kang SK, et al. Hepatoprotective effects of $18{\beta}$-glycyrrhetinic acid on carbon tetrachlorideinduced liver injury: Inhibition of cytochrome P450 2E1 expression. Pharmacol Res 2002;46(3):221-7.   DOI
28 Herbal medicine of Korean Medical College Compilation Commitee. Herbal medicine. Seoul: Younglim Publising Co., Ltd.; 2013, p. 154-5, 635-6, 375-6, 584-5, 528-9, 553-4.
29 Liang HL, Ouyang Q. A clinical trial of combined use of rosiglitazone and 5-aminosalicylate for ulcerative colitis. World J Gastroenterol 2008;14(1):114-9.   DOI
30 Kim JW, Kim CG, Kim HD, Kim BG, Chang DK, Kim JS, et al. The trends of the incidence Rates, Short-term outcome of Treatment and Relapse Rates of Ulcerative Colitis. Korean J Gastroenterol 2003;41(3):15-25.
31 Jujinhyung. Dangyesimbeb. Seoul: Daeseong Publishing Co., Ltd.; 2004, p. 267-84.
32 Specian RD, Oliver MG. Functional biology of intestinal goblet cells. Am J Physiol 1991;260:C183-C93.   DOI