Browse > Article
http://dx.doi.org/10.22246/jikm.2017.38.4.409

Caspase-dependent and Akt-pathway Regulated Rhus Verniciflua Stokes Extract-induced Apoptosis in Human Breast Carcinoma MDA-MB-231 Cells  

Hong, Sang-hoon (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Park, Sang-eun (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Publication Information
The Journal of Internal Korean Medicine / v.38, no.4, 2017 , pp. 409-418 More about this Journal
Abstract
In Korea, Rhus verniciflua Stokes is used to purge hardness, alleviate blood stasis, and treat cancer. However, the mechanisms of related anti-cancer activity are not fully understood in human cancer cells. This study investigated the anti-cancer effects and mechanisms of Rhus verniciflua Stokes on MDA-MB-231 human breast cancer cells and found that treatment with a Rhus verniciflua Stokes extract resulted in time- and concentration-responses that indicated growth inhibition of breast cancer cells by induced apoptosis. This was followed by a decrease in mitochondrial membrane potential; the activation of caspase-3, -8, and -9; and the up-regulation of tBid. Caspase-dependent apoptosis was induced through the inhibition of phosphatidylinositol 3-kinase (PI3K) and the Akt signaling pathway. This study provides evidence that Rhus verniciflua Stokes might be useful for the treatment of breast cancer.
Keywords
Rhus verniciflua stokes; breast cancer; MDA-MB-231 cells; Akt-pathway;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Lee JC, Kim J, Lim KT, Yang MS, Jang YS. Ethanol eluted extract of Rhus verniciflua Stokes showed both antioxidant and cytotoxic effects on mouse thymocytes depending on the dose and time of the treatment. Journal of biochemistry and Molecular Biology 2001;34:250-8.
2 Kitt DD, Lim KT. Antitumorigenic and cytotoxic properties of an ethanol extract derived from Rhus verniciflua Stokes(乾漆). Journal of Toxicology and Environmental Health Part A 2001;64:357-71.   DOI
3 Lee JC, Kim JJ, Jang YS. Ethanol-eluted Extract of Rhus verniciflua Stokes Inhibits Cell Growth and Induces Apoptosis in Human Lymphoma Cells. Journal Biochem Mol biol 2003;4:337-43.
4 Song MS, KIM YS, Lim EM. The Study of Literature Review on Lacas Sinicia Exsiccate. Journal of Oriental Obstetrics ang Gynecology 2002;15(1):60-76.
5 Sun SY, Jr Hail N, Lotan R. Apoptosis as a novel target for cancer chemoprevention. J Natl Cancer Inst 2004;96(9):662-72.   DOI
6 Tibbetts MD, Zheng L, Lenardo M.J. The death effector domain protein family: regulators of cellular homeostasis. Nat Immunol 2003;4(5):404-9.   DOI
7 Kroemer G, Reed JC. Mitochondrial control of cell death. Nat Med 2000;6(5):513-9.   DOI
8 Kroemer G. The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nat Med 1997;3(6):614-20.   DOI
9 Cohen GM. Caspases: the executioners of apoptosis. Biochem J 1997;326:1-16.   DOI
10 Li H, Zhu H, Xu CJ, Yuan J. Cleavage of BID by caspase 8 mediates the mitochondrial damage in the Fas pathway of apoptosis. Cell 1998;94(4):491-501.   DOI
11 Stennicke HR, Salvesen GS. Properties of the caspases. Biochim Biophys Acta 1998;1387(1-2):17-31.   DOI
12 Luo J, Manning BD, Cantley LC. Targeting the PI3K-Akt pathway in human cancer: rationale and promise. Cancer Cell 2003;4(4):257-62.   DOI
13 Engelman JA. Targeting PI3K signalling in cancer: opportunities, challenges and limitations. Nat Rev Cancer 2009;9(8):550-62.   DOI
14 Feng Y, Wang N, Zhu M, Feng Y, Li H, Tsao S. Recent progress on anticancer candidates in patents of herbal medicinal products. Recent Pat Food Nutr Agric 2011;3(1):30-48.   DOI
15 Korea Central Cancer Registry, National Cancer Center. Annual report of cancer stastics in Korea in 2012. Ministry of health and Welfare 2014:19-37.
16 Vogel VG. Selective estrogen receptor modulators and aromatase inhibitors for breast cancer chemoprevention. Curr Drug Targets 2011;12(13):1874-87.   DOI
17 Clarke BL, Khosla S. New selective estrogen and androgen receptor modulators. Curr Opin Rheumatol 2009;21(4):374-9.   DOI
18 Newman DJ, Cragg GM. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod 2012;75(3):311-35.   DOI
19 Qi F, Li A, Inagaki Y, Gao J, Li J, Kokudo N, Li XK, Tang W. Chinese herbal medicines as adjuvant treatment during chemo-or radio-therapy for cancer. Biosci Trends 2010;4(6):297-307.
20 Uezono Y, Miyano K, Sudo Y, Suzuki M, Shiraishi S, Terawaki K. A review of traditional Japanese medicines and their potential mechanism of action. Curr Pharm Des 2012;18:4839-53.   DOI
21 Wang Y, Li Y, Liu X, Cho WC. Genetic and epigenetic studies for determining molecular targets of natural product anticancer agents. Curr Cancer Drug Targets 2013;13(5):506-18.   DOI