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http://dx.doi.org/10.22246/jikm.2017.38.3.353

Effects of Gwaruhaengryeon-hwan on COPD and Particulate Matter Induced Lung Injury on a Mouse Model  

Lee, Chul-wha (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Yang, Won-kyung (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Lyu, Yee-ran (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Kim, Seung-hyeong (Institute of Traditional Medicine and Bioscience, Dae-Jeon University)
Park, Yang-chun (Dept. of Internal Medicine, College of Korean Medicine, Dae-Jeon University)
Publication Information
The Journal of Internal Korean Medicine / v.38, no.3, 2017 , pp. 353-366 More about this Journal
Abstract
Objective: This study aimed to use a mouse model to evaluate the effects of Gwaruhaengryeon-hwan (GHH) on chronic obstructive pulmonary disease (COPD) and particulate matter induced lung injury. Materials and Methods: The study was carried out in two ways (in vitro, in vivo). In vitro RAW 264.7 cells (mouse macrophage) were used and analyzed by flow cytometry, ELISA. In vivo lipopolysaccharide (LPS) and cigarette smoke solution (CSS), or coal, fly ash, diesel exhaust particle (CFD) challenged mice were used and its BALF was analyzed by ELISA, lung tissue by real-time PCR. Results: In vitro, GHH maintained an 80-100% rate of viability. So cytotoxicity was not shown. In the ELISA analysis with RAW 264.7 cells, GHH significantly decreased NO over $30{\mu}g/ml$. In the ELISA analysis, GHH significantly decreased $TNF-{\alpha}$, IL-6 over $300{\mu}g/ml$. In the COPD model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increasing of neutrophils, $TNF-{\alpha}$, IL-17A, MIP2, CXCL-1 in BALF, $TNF-{\alpha}$, $IL-1{\beta}$ mRNA expression in lung tissue and histological lung injury. In the CFD induced lung injury model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increase of neutrophils, $TNF-{\alpha}$, IL-17A, MIP2, CXCL-1 in BALF, MUC5AC, $TGF-{\beta}$ mRNA expression in lung tissue and histological lung injury. Conclusion: This study suggests the usability of GHH for COPD patients by controlling lung tissue injury.
Keywords
Gwaruhaengryeon-hwan; chronic obstructive pulmonary disease; cigarette smoke solution; particulate matter;
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Times Cited By KSCI : 6  (Citation Analysis)
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