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Effects of Jwa Kum-Whan on Reflux Esophagitis in Rats  

Shin, Min-koo (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Kim, Eui-su (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Kim, Tae-ryun (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Lim, Hyun-chan (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Lee, Young-su (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Publication Information
The Journal of Internal Korean Medicine / v.37, no.3, 2016 , pp. 495-507 More about this Journal
Abstract
Objectives: This study was designed to investigate the effects of Jwa Kum-Whan (JKW) on reflux esophagitis in rats.Methods: Forty rats were divided into five groups: a sham group (with no medication and only treated with ventrotoby); a group with reflux esophagitis (RE); a pantoprazole group (treated with 30 mg/kg pantoprazole per day for two weeks); a JKW280 group (treated with 280 mg/kg JKW per day for two weeks); and a JKW560 group (treated with 560 mg/kg JKW per day for 2 weeks). All rats fasted for 24 hrs and then were induced with RE by the oral administration of indomethacin and by a pylorus and forestomach ligation operation. After 8 hrs, the rats were sacrificed. We measured body weight, gastric juice pH, gastric volume, antioxidant activity, and cytokine and made a histologic examination of the esophagus and the stomach.Results: The weights of the rats in each group were not significantly different. The gastric juice pH significantly increased in the JKW560 group and the pantoprazole group compared with the RE group. Gastric volume significantly decreased in the JKW560 group compared with the RE group and the pantoprazole group. SOD activities significantly increased in the JKW280 and JKW560 groups compared with the RE group. Catalase activities significantly increased in the pantoprazole group and the JKW560 group compared with the RE group. TNF-α significantly decreased in the JKW280 and JKW560 groups compared with the RE group. IL-6 significantly decreased in the pantoprazole group and the JKW280 and JKW560 groups compared with the RE group. Histologic examination of the esophagus and the stomach showed significant improvements in the pantoprazole, JKW280, and JKW560 groups compared with the RE group.Conclusion: Based on these results, it is concluded that JKW can prevent reflux esophagitis.
Keywords
Jwa Kum-Whan (Zuojin wan); reflux esophagitis; pantoprazole; gastric volume; catalase; TNF-α ; IL-6;
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1 Kahrilas PJ. GERD pathogenesis, pathophysiology, and clinical manifestations. Cleve Clin J Med 2003;70(suppl 5):4-19.   DOI
2 Seo KW, Yoon KY. Surgical Treatment of Gastroesophageal Reflux Disease. Korean J Gastroenterol 2013;61(3):177-9.   DOI
3 Vakil N, van Zanten SV, Kahrilas P, Dent J, Jones R. The Montreal definition and classification of gastroesophageal reflux disease: a global evidence-based consensus. Am J Gastroenterol 2006;101(8):1900-20.   DOI
4 Lee KJ. Underlying Mechanisms and Management of Refractory Gastroesophageal Reflux Disease. Korean J Gastroenterol 2015;66(2):70-4.   DOI
5 Yoo SS, Lee WH, Ha J, Choi SP, Kim HJ, Kim TH, et al. The Prevalence of Esophageal Disorders in the Subjects Examined for Health Screening. Korean J Gastroenterol 2007;50(5): 306-12.
6 Choi SY. The Role of Diet and Lifestyle Measures in the Pathogenesis and Treatment of Gastroesophageal Reflux Disease. Korean J Gastroenterol 2001;37(3):224-5.
7 Jung HK, Hong SJ, Jo YJ, Jeon SW, Cho YK, Lee KJ, et al. Updated Guidelines 2012 for Gastroesophageal Reflux Disease. Korean J Gastroenterol 2012;60(4):195-218.   DOI
8 Kim JH, Kim BJ, Kim SW, Kim SE, Kim YS, Sung HY, et al. Current Issues on Gastroesophageal Reflux Disease. Korean J Gastroenterol 2014; 64(3):127-32.   DOI
9 The co-textbook publishing committee of Korean oriental medicine school. internal medicine of spleen. Seoul: Koonja press; 2008, p. 317.
10 Kim HJ, Lim SY, Kwak MA, Kim DJ, Byun JS. Effects of Yijintang-gamibang on Reflux Esophagitis Induced by Pylorus and Forestomach Ligation in Rat. Korean J Orient Int Med 2010;31(1):128-41.
11 Jang MW, Lim SW. Experimental Study for Effect of Banhasasim-tang on Mice with Reflux Esophagitis. Korean J Orient Int Med 2013; 34(4):362-74.
12 Lee YJ, Park JH, Roh SS. Effects on Rats with Reflux Esophagitis Treated with Lonicerae Flos Extract. Korean J Oriental Physiology & Pathology 2010;24(6):970-5.
13 Kim DJ, Roh SS. Effect on Acute reflux Esophagitis by Evodiae Fructus Aquous Extract. Kor J Herbology 2012;27(1):51-8.   DOI
14 Ju JH. Dangyeeuijip. Beijing: Inminwisaeng press; 1995, p. 219, 470.
15 The co-textbook publishing committee of Korean oriental medicine school. The herbal medicine. Seoul: Younglimsa; 2008, p. 218-9, 379, 380.
16 Choi GY, Sun SH, Kim BW, Lee SJ, Oh HS, Han IS, et al. Literature Review on Syndrome Differentiation and Herbal Medicine of Reflex Esophagitis -Focusing on Chinese Journals-. Korean J Orient Int Med 2011;32(1):75-86.
17 Wang J, Zhang T, Zhu L, Ma C, Wang S. Anti-ulcerogenic effect of Zuojin Pill against ethanol-induced acute gastric lesion in animal models. J Ethnopharmacol 2015;173:459-67.   DOI
18 Peng QX, Cai HB, Peng JL, Yung KL, Shi J, Mo ZX. Extract of Zuojin Pill(左金丸) induces apoptosis of SGC-7901 cells via mitochondria-dependent pathway. Chin J Integr Med 2015; 21(11):837-45.   DOI
19 Chen X, Wan ZQ, Han GC, Wang JD, Zhao Z, Zhou P. [Study on efficacy of liujunzi decoction combined with zuojin pills in treating acute radioactive duodenitis and their mechanism]. Zhongguo Zhong Yao Za Zhi 2014;39(2):278-84.
20 Jeon SA. Antibiotic, Antioxidant, Whitening effects of Jwa Kum-Whan and Soo Ryeon-Whan. Graduate School of Wonkwang University 2014.
21 Seong MJ. Anti-inflammatory effect of Joakeumwhan. Graduate School of Wonkwang University 2008.
22 Fass R, Gasiorowska A. Refractory GERD: what is it?. Curr gastroenterol Rep 2008;10(3): 252-7.   DOI
23 Kim YH, Seng NS, Lee YJ. The Effects of Jwa Kum-Whan and Soo Ryeon-Whan on the Hyperlipidemia in Rats. Kor J Herbology 2005; 20(2):91-102.
24 Choi DH, Moon G, Moon SJ. Effects of Joakeumwhan(左金丸) Extract on Motility of Intestine and Gastric Secretion. The Journal of Wonkwang Oriental Medicine 2005;3(1): 133-47.
25 Choi OC, Lai YS, Park SD. Effect of Joagumhwan and its components on acetaminophen induced hepatoxicity in rats. Kor J Herbology 2004;19(4):67-79.
26 Shukla K, Raj P, Kumar A, Kumar M, Kaithwas G. Effect of Monotherapy and Combination Therapy of Pantoprazole and Aprepitant in Gastric Esophageal Reflux Disease in Albino Rats. Scientific World Journal 2014; 2014:1-7.
27 Choi BH, Kwak MA, Kim DJ, Byun JS. Suppressive Effects of Yijintang-gamibang on Reflux Esophagitis. J Korean Oriental Med 2010;31(5):64-81.
28 Hwang IK. Establishment of Functionality Evaluation System for Health Functional Food on Gastrointestinal Tract. Seoul: Graduate school of Seoul National University; 2003, p. 69-70.
29 Choi MS. Antimicrobial Effects of Scutellaria baicalensis, Coptis chinensis, and Phellodendron amurense. Graduate School of Small Business Soongsil University 2010.
30 Yoon KR, Kim YJ, Lee E, Lee JM. Anti-inflammatory Effect of Coptidis Rhizoma. Kor J Herbology 2009;24(3):79-86.
31 Kim YJ, Lee MJ, Park JW, Kim JK, Choi DY, Kim CH. Antioxidant Activity of Water-Extract from Coptis chinensis Franch. Korean Journal of Life Science 2000;10(3):241-6.
32 Lee HJ. Anti-oxidative activity of evodiae fructus MeOH extract. Graduate school of Chung-ang University 2007.
33 Locke GR, Talley NJ, Fett SL, Zinsmeister AR, Melton LJ. Risk factors associated with symptoms of gastroesophageal reflux. Am J Med 1999;106(6):642-9.   DOI
34 Kum SY. Anti-inflammatory and anti-migration effect of methanol extract of Evodia officinalis DODE in HASMC. Graduate school of Dongguk University 2009.
35 Jeon ES, Han MD, Kim HD. Antimicrobial Activity of Streptococcus mutans by Schizandrae fructus and Evodiae fructus extracts. Journal of Dental Hygiene Science 2003;3(1);39-44.
36 Papadakis MA, McPhee SJ, Rabow MW. Current Medical Diagnosis & Treatment. New York: McGraw-Hill Education; 2015, p. 590-5.
37 DeMeester TR, Wernly JA, Bryant GH, Little AG, Skinner DB. Clinical and in vitro analysis of determinants of gastroesophageal competence: a study of the principles of antireflux surgery. Am J Surg 1979;137(1):39-46.   DOI
38 Hunt RH. Importance of pH control in the management of GERD. Arch Intern Med 1999;159(7):649-57.   DOI
39 Gaynor EB. Otolaryngologic manifestations of gastroesophageal reflux. Am J Gastroenterol 1991;86(7):801-8.
40 Wetscher GJ, Hinder RA, Bagchi D, Hinder PR, Bagchi M, Perdikis G, et al. Reflux esophagitis in humans is mediated by oxygen-derived free radicals. Am J Surg 1995;170(6) :552-7.   DOI
41 Rao CV, Vijayakumar M. Effect of quercetin, flavonoids and alpha-tocopherol, an antioxidant vitamin on experimental reflux oesophagitis in rats. Eur J Pharmacol 2008; 589(1):233-8.   DOI
42 Cho SY. Effects of platycodin D on the reflux esophagitis by modulating antioxidant defense systems. Graduate School of Daegu Haany University 2014.
43 Nagahama K, Yamato M, Nishio H, Takeuchi K. Essential role of pepsin in pathogenesis of acid reflux esophagitis in rats. Dig Dis Sci 2006;51(2):303-9.   DOI
44 Rieder F, Cheng L, Harnett KM, Chak A, Cooper GS, Isenberg G, et al. Gastroesophageal reflux disease-associated esophagitis induces endogenous cytokine production leading to motor abnormalities. Gastroenterology 2007;132(1): 154-65.   DOI
45 Straumann A, Bussmann C, Conus S, Beglinger C, Simon HU. Anti-TNF-α(infliximab) therapy for severe adult eosinophilic esophagitis. J Allergy Clin Immunol 2008;122(2):425-7.   DOI
46 Longo D, Fauci A, Kasper D, Hauser S, Jameson J, Loscalzo J. Harrison's Principles of Internal Medicine 18th edition. New York: McGraw-Hill Professional; 2011, p. 1906-8.