Browse > Article

Single Oral Dose Toxicity Study and Aconitine Content Analysis of Raw Aconiti Tuber and Sambu-tang  

Jeong, Hae-ryong (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Son, Byun-woo (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Kim, Gyeong-cheul (Dept. of Diagnostics, College of Korean Medicine, Dong-Eui University)
Shin, Soon-shik (Dept. of Presciptionology, College of Korean Medicine, Dong-Eui University)
Hwang, Won-deuk (Dept. of Korean Internal Medicine, College of Korean Medicine, Dong-Eui University)
Publication Information
The Journal of Internal Korean Medicine / v.37, no.3, 2016 , pp. 427-441 More about this Journal
Abstract
Objectives: This study investigated the aconitine contents analysis of Buja extracts (raw material of Buja, hot water extract of Buja, and hot water extract of Sambu-tang) and the single oral dose toxicity of Sambu-tang-R in six-week-old Sprague-Dawley rats in order to compare the toxicity of Buja extracts.Methods: Aconitine content analysis is that Buja extracts were hold purity test. To detect single oral dose toxicity, six-week-old Sprague-Dawley rats were divided into two groups, a normal control group and a sambutang-R (2,000 mg/kg) group. For 14 days of treatment, clinical signs, body weight, clinical chemistry, necropsy, and histopathology were examined.Results: The aconitine contents of the Buja extracts were Buja-RH (0.1738%), Buja-RD (0.1746%), and Sambu-tang-R (0.0961%). There were no cases of death in either the control group or the experimental group. Nor was there any disorder to the clinical signs or any significant change in body weight in either group. There was no significant change of clinical chemistry or disorder of necropsy findings in either the control or the experimental group. And there was no difference in histopathological findings in comparing the control group with the experimental group.Conclusions: These results suggest that the aconitine content of the hot water extract of Buja was similar to the raw material of Buja, but the hot water extract of Sambu-tang had greatly decreased aconitine content. These results also suggest that a single oral lethal dose of Sambu-tang-R for Sprague-Dawley rats exceeds 2,000 mg/kg for both female and male rats.
Keywords
aconitine; Buja-RH (raw-material of Buja); Buja-RD (hot-water extract of Buja); Sambu-tang-R (hot-water extract of Sambu-tang); single-oral-dose-toxicity;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
연도 인용수 순위
1 Park HM, Jang IS, Lee SD. Hepatotoxic Events Associated with Herbal Medicinal Products, Folk Remedies and Food Supplements in Korea. J Korean Oriental Med 2005;26(2):152-65.
2 Jang IS, Yang CS, Lee SD, Han CH. A Review of Herbal Medicinal Products Associated with Toxic Events in Korea. J Korean Oriental Med 2007;28(1):1-10.
3 Lee E, Park BW, Hea GJ, Ko H. A Study on The Side Effects and Toxicity of Herbal Medicine. Korean J Orient Int Med 2002;23(2):222-7.
4 Kang BS, Kim IR, Kim HC, Guk YB, Park YG, Seo BI, et al. Bonchohak. Seoul: Yeonglimsa; 2007, p. 372-4, 572-5.
5 Shin MK. Imsang-bonchohak. Seoul: Yeonglimsa; 2000, p. 188-91, 298-304.
6 Shin GG. Shinci-bonchohak(各論). Seoul: Gomunsa; 1988, p. 1-8, 23-9.
7 Kang BS, Kim YP. Imsangbaehap-bonchohak. Seoul: Yeonglimsa; 1996, p. 108-12, 193-6.
8 Ahn SY, Kim SI. Bonchojeongeui. Seoul: Cheonghong; 2009, p. 45-65, 540-53.
9 Shim YG. Jungyak-pharmacology. Beijing: Inminwiseng; 2000, p. 490-6.
10 Seo BI, Jeong GY. Easily learning bonchohak. Gyeongsan: Daeguhani-university publishing department; 2007, p. 209-12.
11 Joe CH. Jungchoyakjungdokgeupgu. Seongdojeongigongjeong hakwon; 1989, p. 68-9.
12 Lee JS, Yun YG. A Study on the Applications of Radix Aconiti Lateralis Prepararta Mainly Blended Prescription in Dongeuibogam. Master's thesis of Wonkwang University Graduate School 2010;1-38.
13 Song IS, Oh MS, Song TW. The study of Acontii Tuber in Bang-Yak-Hap-Pyun. Daejeon University Institute of Oriental Medicine 2001; 10(1):323-36.
14 Lee HH, Ryu SJ. The investigation about the Seokgok’s use of Rehmanniae Radix and Aconiti Iateralis Preparata Radix. J Somun Oriental Medical Society 2007;10(1):75-82.
15 Lee B. Bangjaehak(Sangchec). Beijing: Inminwiseng. 2011, p. 676.
16 Jang JW, Kim JY, Min SS, Song SJ, Kim KT, Hwang WD. The Consequences of Long-period Dosages of Herbal Medicine Including Boo-ja and Cheon-o on Liver Injury. Korean J Orient Int Med 2005;26(4):828-35.
17 Song SJ, Jang JW, Hwang WD. The Consequences of Long-period Dosages of Herbal Medicine Including Boo-ja and Cheon-o on Liver Injury (Ⅱ). Korean J Orient Int Med 2006;27(2): 407-15.
18 Han HY, Hwang WD. The Study of Dosages of Herbal Medicine Including Aconiti Lateralis Preparata Radix and Aconiti Tuber on Aspartate Aminotransferase, Alanine Aminotransferase. Kor J Herbology 2009;24(4):87-93.
19 Lee YJ, Kim KG, Hwang WD, Lee JW, Jeong HJ, Bae SH. The Study of Safety of Herbal Medicine Including Fuzi(Buja) on High Range of LFT, RFT Patients. The Korean Journal of Oriental Medical Prescription 2011;19(2):179-89.
20 Ahn SY, Hwang WD. The Consequences of Dosage of Herbal Medicine Including Buja and Cheono on Electrocardiography. Master's thesis of Dongeui University Graduate School 2015;1-28.
21 Kang BS, Seo BI, Choi HY. Herbal medicine processing and clinical application. Seoul: Yeonglimsa; 2003.
22 Kim YK, Lee JH, Song KY, Park SK, Kim CS. Studies on the Liver Toxicities with different Dosage of Wild Aconiti Tuber Decoction. The Korean Journal of Oriental Medical Prescription 2005;13(1):123-43.
23 Lim TH, Park KY. A case of aconitine poisoning with transient tetraplegia and hypocalcemia. Korean Journal of Medicine 1998;55(6):1093-6.
24 Park HS, Kim SH, Kim PY, Chang IM. Toxicological Studies on Raw and Processed Aconiti Tubers; Acute, Subacute Toxicity Studies and Assay of Aconitine Alkaloids. Korean J Toxicol 1990;6(1):41-9.
25 Lee JH. Effects on Gene Expressions in the Liver and Transition Ratio of Cytotoxic Alkaloid Components in Herbal Prescriptions Containing Aconitum Species. Doctor's thesis of Wonkwang University Graduate School 2006;1-76.
26 Hikino H, Yamada C, Nakamura K, Sato H, Ohizumi Y, Endo K. Change of Alkaloid Composition and Acute toxicity of Aconitum Roots during Processing. Yakugaku Zasshi 1977; 97(4):359-66.   DOI