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Association Study in Endothelin 1 (EDN1) Gene Polymorphism and Excess or Deficiency Syndrome in Korean Asthmatic Patients  

Yoem, Yu-rim (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Kim, Kwan-il (Korean Medicine Clinical Trial Center, Kyung-Hee University Korean Medicine Hospital)
Baek, Hyun-jung (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Kim, Mi-a (Dept. of Cardiovascular and Neurologic Diseases (Stroke center), College of Korean Medicine, Kyung-Hee University)
Lee, Beom-joon (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Kim, Jin-ju (Dept. of Oriental Physiology, College of Pharmacy, Kyung-Hee University)
Kim, Su-kang (Dept. of Pharmacology, College of Medicine, Kyung-Hee University)
Chung, Joo-ho (Dept. of Pharmacology, College of Medicine, Kyung-Hee University)
Jung, Hee-jae (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Jung, Sung-ki (Dept. of Clinical Korean Medicine, Graduate School, Kyung Hee University)
Publication Information
The Journal of Internal Korean Medicine / v.37, no.1, 2016 , pp. 47-64 More about this Journal
Abstract
Objectives: In the present study, a genetic analysis was conducted to investigate the association of the expression of SNPs of EDN1 gene polymorphism with the clinical phenotype in bronchial asthma patients with either excess or deficiency syndrome.Methods: Ninety-four healthy control subjects and 52 asthma patients were included in this study. The asthma patients were divided into two groups: those with deficiency syndrome and those with excess syndrome. We searched the exonic and promoter areas of the EDN1 gene in the NCBI website SNPs with <0.01 minor allele frequency (MAF) and <0.01 heterozygosity. Pro programs were performed to obtain the odds ratio, 95% confidence interval, and p-value. Multiple logistic regression models were conducted to analyze the genetic data.Results: In our genotype and allele analyses, there were significant differences in the codominant 2 model of the rs3087459 SNP genotype and also in the CGG haplotype between the control group and the asthma group. Genotype and allele analyses were conducted between the deficiency and excess syndrome group. There were significant differences in the dominant and log-additive model and also in the frequency of C-alleles of rs3087459 SNP genotype. There were significant differences in codominant 1, dominant and log-additive model and T-allele of rs5370 SNP genotype. The AGG haplotype also revealed significant differences.Conclusions: EDN1 SNPs (rs3087459, rs5370) showed a significant association with symptomatic excess syndrome in Korean asthmatic patients.
Keywords
EDN1; polymorphism; asthma; excess syndrome; deficiency syndrome;
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