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Characteristic Effects of Dangnyo-hwan for Diabetes Control Studied Using LC-MS/MS and ICP  

In, Jeong-do (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dong-Eui University)
Im, Dai-sig (Dept. of Chemistry, College of Natural Sciences, Soonchunhyang Universi)
Moon, Seung-ho (R&D Center, IC)
Ki, Won-ill (Dept. of Oriental Internal Medicine, College of Oriental Medicine, Dong-Eui University)
Publication Information
The Journal of Internal Korean Medicine / v.36, no.3, 2015 , pp. 217-227 More about this Journal
Abstract
Objectives: The primary aim of this study was to identify bioactive compounds in Dangnyo-hwan, a Korean herbal medicine, through instrumental analysis using LC-MS/MS and ICP, and investigate its potential use in diabetes treatment. Methods: The extract of Dangnyo-hwan has 12 medicinal herbs; these were compared with 18 marker substances selected from literature survey. Results: LC-MS/MS analysis could detect 9 of the 18 bioactive compounds: citruline, catalpol, berberine, ginsenoside Rb1, ginsenoside Rg1, oleanolic acid, β-sitosterol, mangiferin, and schizandrin. While harmful heavy metals such as As, Pb, Cd, Hg, Ni, and Cu were not present in high concentrations, Zn concentration was 4.2 mg in 100 g Dangnyo-hwan. Conclusions: Instrumental analysis such as LC-MS/MS and ICP was successfully used to identify bioactive compounds in Dangnyo-hwan. Detection of 9 bioactive substances and Zn from the herb medicine is a valuable finding, and suggests that Dangnyo-hwan is a candidate medicine for diabetes. Further investigations like in vitro assay, percent GPR 119 activity, and percent human DGAT-1 inhibition are underway.
Keywords
Dangnyo-hwan; diabetes; LC-MS/MS; ICP; marker substances;
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1 Keen CL, Gershwin ME. Zinc deficiency and immune function. Annual review of nutrition 1990;10:415-31.   DOI
2 Huang WJ, Niu HS, Lin MH, Cheng JT, Hsu FL. Antihyperglycemic effect of catalpol in streptozotocin-induced diabetic rats. J Nat Prod 2010;73:1170-2.   DOI
3 윤미소, 김도윤, 유호진, 박주훈, 장상희, 원경종, 김보경, 이환명. Schizandrin의 신혈관형성억제에 의한 항암효과. 동의생리병리학회지 2012;26(5):687-92.
4 Chang R, Li Y, Yang X, Yue Y, Dou L, Wang Y, Zhang W, Li X. Protective role of deoxyschizandrin and schisantherin a against myocardial ischemia-reperfusion injury in rats. PloS one 2013;8(4):e61590.   DOI
5 Lu WQ, Qiu Y, Li TJ, et al. Timosaponin B-II inhibits pro-inflammatory cytokine induction by lipopolysaccharide in BV2 cells. Arch Pharm Res 2009;32:1301-8.   DOI
6 Muruganandan S, Srinivasan K, Gupta S, Gupta PK, Lal J. Effect of mangiferin on hyperglycemia and atherogenicity in streptozotocin diabetic rats. Journal of Ethnopharmacology 2005;97:497-501.   DOI
7 Lee HA, Kwon SO, Lee HB. Hypoglycemic action of components from red ginseng: (I). Investigation of the effect of ginsenosides from red ginseng on enzymes related to glucose metabolism in cultured rat hepatocytes. Journal of Ginseng Research 1997;21:174-86.
8 Lee HA, Sim HS, Choi KJ, Lee HB. Hypoglycemic action of red ginseng components (II): investigation of the effect of fat soluble fraction from red ginseng on enzymes related to glucose metabolism in cultured rat hepatocytes. Journal of Ginseng Research 1998;22:51-9.
9 Yamasaki K. Effect of some saponins on glucose transport system. Book of Abstracts, 21th ACS National Meeting, Part 1. Chicago: American Chemical Society; 1995, p. 223.
10 Milbury PE, Richer AC. Understanding the Antioxidant Controversy: Scrutinizing the “fountain of Youth". London: Greenwood Publishing Group;2008, p. 99.
11 신민교. 臨床本草學. 서울: 永林社; 2012, p. 188, 252, 265, 280, 368, 369, 402, 505, 619, 650, 717, 806.
12 Yoon SH, Ha H. The Effect of Bombycis corpus on Streptozotocin - induced Diabetic Rats. J Korean Soc Hygienic Sciences 2006;6(1):11-22.
13 Zhou J, Zhou S, Tang J, et al. Protective effect of berberine on beta cells in streptozotocin and high-carbohydrate/high-fat diet-induced diabetic rats. Eur J Pharmacol 2009;606:262-8.   DOI
14 Cong L, Cao G, Renyu X, Zhonghua P, Xiaojian Z, Zhou W, Gong C. Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms. Biochem Biophys Res Commun 2011;410(4):721-5.   DOI
15 Yamahara J, Mibu H, Sawada T, Fujimura H, Takino S, Yoshikawa M, Kitagawa I. Biologically active principles of crude drugs. Antidiabetic principles of Corni fructus in experimental diabetes induced by streptozotocin. Yakugaku Zasshi 1981;101:86-90.   DOI
16 Lin G, Xu XD, Liu D, Ju JH, Yang JS, Study on chemical constituents of Aralia decaisneana. Chinese Pharmaceutical Journal 2000;35:298-300.
17 Liu L, Yu YL, Yang JS, et al. Berberine suppresses intestinal disaccharidases with beneficial metabolic effects in diabetic states, evidences from in vivo and in vitro study. Naunyn Schmiedebergs Arch Pharmacol 2010;381:371-81.   DOI
18 Pan GY, Huang ZJ, Wang GJ, Fawcett JP, Liu XD, Zhao XC, Sun JG, Xie YY. The antihyperglycaemic activity of berberine arises from a decrease of glucose absorption. Planta Medica 2003;69:632-6.   DOI
19 Cox M, Lehninger AL, Nelson DR. Lehninger principles of biochemistry. 3rd ed. W. H. Freeman and Company; 2000, p. 92-3.
20 Park EH, Kahng JH, Lee SH, Shin KH. Ananti-inflammatory principle from cactus. Fitoterapia 2001;72:288-90.   DOI
21 보건복지부통계포털:통계포털-통계발간물-주제별발간물-질병건강-2013국민건강통계. 54-55.http://stat.mw.go.kr/front/statData/publicationView.jsp?menuId=47&bbsSeq=13&nttSeq=21549&searchKey=&searchWord=&nPage=1&topSelect=B00005
22 유영법, 김미정, 황대선, 하혜경, 마진열, 신현규. HPLC-MS/MS에 의한 사물탕의 지표성분 분석. 대한본초학회지 2007;22(2):97-102.
23 전국한의과대학 신계내과학교실. 신계내과학. 서울: 군자출판사; 2011, p. 89-96, 301-12.
24 당뇨병 진료지침. 대한당뇨병학회. 2013.
25 김도형, 박승찬, 이지혜, 이혜윤, 조민경, 최준용, 김소연, 박성하. 당뇨병의 한의학적 치료에 대한 최신 연구 동향 - 2008년부터 2013년까지 국내학술논문을 중심으로 -. 대한한방내과학회지 2013;34(3):240-55.
26 박성진, 성동호, 박동식, 김승섭, 고징유, 안주희, 외. 발효더덕의 화학성분. 한국식품영양학회지 2009; 38(3):396-400.
27 성기천. 천연 강황 추출물의 약리화학적 특성 및 분석. 한국유화학회지 2011;28(4):393-401.   DOI
28 서창섭, 신현규. LC-MS/MS를 이용한 당귀수산 추출물 중 17종 성분의 함량분석. 약학회지 2014; 8(3):158-64.
29 황영명, 유영법, 김종열, 고병희, 이의주. 태음인 가미조위탕의 HPLC 분석. 사상체질의학회지 2006;8(1):147-54.
30 Xie W, Du L. Diabetes is an inflammatory disease: evidence from traditional Chinese medicines. Diabetes, Obesity and Metabolism 2011;13:289-301.   DOI
31 Li WL, Zheng HC, Bukuru J, De Kimpe N. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J of Ethnopharmacology 2004;92:1-21.   DOI