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Protective Effects of Sosokmyoung-tang Against Parkinson's Model in Human Neuroblastoma SH-SY5Y Cells  

Woo, Chan (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
You, Ju-Yeon (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Jang, Chul-Yong (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Kim, Hyo-Rin (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Shin, Yong-Jeen (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Moon, A-Ji (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Shin, Sun-Ho (Dept. of Internal Medicine, College of Oriental Medicine, Won-Kwang University)
Publication Information
The Journal of Internal Korean Medicine / v.35, no.3, 2014 , pp. 298-308 More about this Journal
Abstract
Objectives: In this study we made an effort to investigate the protective effect of SSMT on the N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) -induced cytotoxicity of SH-SY5Y cells. Methods: The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MMT) assay. The fluorescence intensity was measured by using a dye and then with propidium iodide (PI) DNA flow cytometry analysis of the effects on the cell cycle of the SH-SY5Y cells and were used to measure the fluorescence of intracellular reactive oxygen species generation by MPTP. Results: Pretreatment of SSMT significantly suppressed MPTP-induced cytotoxicity, which was revealed as apoptosis characterized by the reduction of cell viability, the increase of ROS production, and the loss of mitochondrial membrane potential in SH-SY5Y cells. Conclusions: These findings suggest that SSMT exerts neuroprotective effects on human neuroblastoma SH-SY5Y cells by MPTP-induced dopaminergic neurodegeneration.
Keywords
SH-SY5Y cell; neuroprotective effect; Sosokmyoung-tang (SSMT);
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Times Cited By KSCI : 2  (Citation Analysis)
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