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Effect of Coptidis Rhizoma Steamed with Rice Wine on Gastroduodenal Mucosa of Mouse through Inhibiting iNOS Activation  

Kim, Myung-Ho (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Guk University)
Lim, Seong-Woo (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Guk University)
Publication Information
The Journal of Internal Korean Medicine / v.35, no.3, 2014 , pp. 262-273 More about this Journal
Abstract
Objectives: This study was carried out to investigate the protective effect of Coptidis Rhizoma steamed with rice wine (CR) against gastroduodenal mucosal injury through inhibiting inducible nitric oxide synthase (iNOS) activation. Methods: In in vitro experiment, LPS-induced RAW 264.7 macrophages were treated with CR(0.4, 0.6, 0.8, 1.0 mg/ml) and iNOS mRNA expression and nitric oxide (NO) production were measured. In in vivo experiment normal group mice were treated with neither ethanol nor CR. Both control and sample group mice were orally administrated with ethanol. Five hours after ethanol administration control group mice were orally administrated with distilled water, sample group mice were orally administrated with CR. After three days administration, gastroduodenal mucosa of mice was observed histopathologically and iNOS, nuclear factor-kappa B (NF-${\kappa}B$) activation were observed immunohistochemically. Results: In in vitro experiment iNOS mRNA expression and NO production in LPS-induced RAW 264.7 macrophages were decreased by CR dose-dependently. In in vivo experiment, gastroduodenal mucosal injury was repaired by CR and iNOS, NF-${\kappa}B$ activation in gastroduodenal mucosa were decreased by CR. Conclusions: Coptidis Rhizoma steamed with rice wine has a protective effect against gastroduodenal mucosal injury through inhibiting iNOS activation.
Keywords
Coptidis Rhizoma steamed with rice wine; Gastroduodenal Ulcer; Nitric Oxide; Inducible Nitric Oxide Synthase; Nuclear Factor kappa B;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 대한내과학회 해리슨내과학 편집위원회. Harrison's 내과학 16th edition. 서울: 도서출판 MIP; 2006, p. 1907.
2 한의과대학 본초학 편찬위원회. 본초학. 초판. 서울: 도서출판 영림사; 2006, p. 221-2.
3 Kim KB, Lee HT, Ku KH, Hong JW, Cho SI. Review of pharmacological effects of Coptidis Rhizoma and its bioactive compounds. J Korean Oriental Med 2012;33(3):160-83.
4 Li B, Shang JC, Zhou QX. Study of total alkaloids from Rhizoma Coptis Chinensis on experimental gastric ulcers. Chin J Integr Med 2005;11(3):217-21.   DOI   ScienceOn
5 전국한의과대학 비계내과학교실. 비계내과학. 초판. 서울: 군자출판사; 2008, p. 79, 213.
6 Byun JS. Protective effects of Coptidis Rhizoma on ethanol-induced gastric ulcer in mice. Korean J Oriental Physiogy & Pathology 2012;26(1):67-73.
7 Lu JS, Liu YQ, Li M, Li BS, Xu Y. Protective effects and its mechanisms of total alkaloids from rhizoma Coptis chinensis on Helicobacter pylori LPS induced gastric lesion in rats. Zhongguo Zhong Yao Za Zhi 2007;32(13):1333-6.
8 허준 저. 윤석희 김형준, 외 옮김. 동의보감. 초판. 경남: 동의보감 출판사; 2006, p. 140, 2157.
9 Li JC, Shen XF, Meng XL. A traditional Chinese medicine JiuHuangLian (Rhizoma coptidis steamed with rice wine) reduces oxidative stress injury in type 2 diabetic rats. Food Chem Toxicol 2013 Sep;59:222-9.   DOI   ScienceOn
10 Nussler AK, Billiar TR. Inflammation, immunoregulation, and inducible nitric oxide synthase. J Leukoc Biol 1993;54(2):171-8.
11 Elliott SN, Wallace JL. Nitric oxide: a regulator of mucosal defense and injury. J Gastroenterol 1998;33(6):792-803.   DOI
12 Park YK, Jung HW, Kim CM, Choi JS, Kim YS. Effects of Berberine on the production of inflammatory mediators from LPS-stimulated BV2 microglial cells. The Korea Journal of Herbology 2007;22(4):117-25.
13 Zhang Q, Piao XL, Piao XS, Lu T, Wang D, Kim SW. Preventive effect of Coptis chinensis and berberine on intestinal injury in rats challenged with lipopolysaccharides. Food Chem Toxicol 2011;49(1):61-9.   DOI   ScienceOn
14 Park YK, Lee KY. Anti-inflammatory effect of chloroform fraction of Coptidis rhizoma on the production of inflammatory mediators from LPS-stimulated BV2 microglial cells. The Korea Journal of Herbology 2007;22(4):109-16.
15 Aktan F. iNOS-mediated nitric oxide production and its regulation. Life Sciences 2004;75(6):639-53.   DOI   ScienceOn
16 Xie QW, Kashiwabara Y, Nathan C. Role of Transcription Factor NF-${\kappa}B/Rel$ in Induction of Nitric Oxide Synthase. J Biol Chem 1994; 269(7):4705-8.
17 Jung HW, Park YK. Inhibitory effects of Coptidis Rhizoma on the LPS-induced production of nitric oxide and TNF-${\alpha}$ in mouse macrophage cells. The Korea Journal of Herbology 2006;21(2):165-73.
18 Jung HW, Park YK. Effects of subfractions of Coptidis Rhizoma extract on the nitric oxide production in LPS-stimulated BV2 microglial cells. The Korea Journal of Herbology 2007;22(2):73-8.
19 Tak PP, Firestein GS. NF-${\kappa}B$: a key role in inflammatory diseases. J Clin Invest 2001;107(1):7-11.   DOI   ScienceOn
20 van Den Brink GR, ten Kate FJ, Ponsioen CY, Rive MM, Tytgat GN, van Deventer SJ, et al. Expression and activation of NF-${\kappa}B$ in the antrum of the human stomach. J Immunol 2000;164(6):3353-9.   DOI
21 Neurath MF, Fuss I, Schurmann G, Pettersson S, Arnold K, Muller-Lobeck H, et al. Cytokine gene transcription by NF-kappa B family members in patients with inflammatory bowel disease. Ann N Y Acad Sci 1998;859:149-59.   DOI
22 Moorchung N, Srivastava AN, Sharma AK, Achyut BR, Mittal B. Nuclear factor kappa-B and histopathology of chronic gastritis. Indian J Pathol Microbiol 2010;53(3):418-21.   DOI   ScienceOn
23 Zhang Q, Piao XL, Piao XS, Lu T, Wang D, Kim SW. Preventive effect of Coptis chinensis and berberine on intestinal injury in rats challenged with lipopolysaccharides. Food and Chemical Toxicology 2011;49(1):61-9.   DOI   ScienceOn
24 Prasad K, Prabhu GK. Image analysis tools for evaluation of microscopic views of immunohistochemically stained specimen in medical research-a review. J Med Syst 2012;36(4):2621-31.   DOI   ScienceOn
25 Knoll MR, Kolbel CB, Teyssen S, Singer MV. Action of pure ethanol and some alcoholic beverages on the gastric mucosa in healthy humans: A descriptive endoscopic study. Endoscopy 1998;30(3):293-301.   DOI   ScienceOn
26 Lee JS, Park HK, Woo ER, Lee EJ, Ahn DK, Youn WS, Shin KH. A study on the Extraction Efficiency of berberine in Coptidis Rhizoma. Analytical Science & Technology 1997;10(2):83-90.
27 Gottfried EB, Korsten MA, Lieber CS. Alcohol-induced Gastric and Duodenal Lesions in Men. Am J Gastroenterol 1978;70(6):587-92.
28 McManus JFA. Histological and Histochemical Uses of Periodic Acid. Stain Technol 1948;23(3):99-108.
29 Bry L, Falk P, Huttner K, Ouellette A, Midtvedt T, Gordon JI. Paneth cell differentiation in the developing intestine of normal and transgenic mice. Proc Natl Acad Sci USA 1994;91(22):10335-9.   DOI