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Research on Anti-lipogenic Effect and Underlying Mechanism of Laminaria japonica on Experimental Cellular Model of Non-alcoholic Fatty Liver Disease  

Kim, So-Yeon (School of Korean Medicine, Pu-san National University)
Kwon, Jung-Nam (School of Korean Medicine, Pu-san National University)
Lee, In (School of Korean Medicine, Pu-san National University)
Hong, Jin-Woo (School of Korean Medicine, Pu-san National University)
Choi, Jun-Yong (School of Korean Medicine, Pu-san National University)
Park, Seong-Ha (School of Korean Medicine, Pu-san National University)
Kwun, Min-Jung (School of Korean Medicine, Pu-san National University)
Joo, Myung-Soo (School of Korean Medicine, Pu-san National University)
Han, Chang-Woo (School of Korean Medicine, Pu-san National University)
Publication Information
The Journal of Internal Korean Medicine / v.35, no.2, 2014 , pp. 175-183 More about this Journal
Abstract
Objectives : We tried to uncover the anti-lipogenic effect and underlying mechanism of Laminaria japonica on an experimental cellular model of non-alcoholic fatty liver disease. Methods : Ethanol extract of Laminaria japonica (LJ) was prepared. Intracellular lipid content of palmitate-treated HepG2 cells was evaluated with or without LJ treatment. We measured the effects of LJ on liver X receptor ${\alpha}$ ($LXR{\alpha}$) and sterol regulatory element-binding transcription factor-1c (SREBP-1c) expression, transcription level of lipogenic genes, including acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), stearoyl-CoA desaturase-1 (SCD-1), and nuclear factor erythroid 2-related factor 2 (Nrf2) activation in HepG2 cells. Results : LJ markedly attenuated palmitate-induced intracellular lipid accumulation in HepG2 cells. LJ suppressed $LXR{\alpha}$-dependent SREBP-1c activation, and SREBP-1c mediated induction of ACC, FAS, and SCD-1. Furthermore, LJ activated Nrf2, which plays an important cytoprotective role in non-alcoholic fatty liver disease. Conclusions : Our study suggests that LJ has the potential to alleviate hepatic lipid accumulation, and this effect was mediated by inhibiting the $LXR{\alpha}$-SREBP-1c pathway that leads to hepatic steatosis. In addition, the anti-lipogenic potential may, at least in part, be associated with activation of Nrf2.
Keywords
Laminaria japonica; non-alcoholic fatty liver disease; $LXR{\alpha}$; SREBP-1c; Nrf2;
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