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Effect of Gagam-Danguieumja through Regulation of MAPK on LPS-Induced Inflammation in RAW 264.7 Cells  

Kim, Tae-Yeon (Dept. of Oriental Pathology, College of Korean Medicine, Se-Myung University)
Publication Information
The Journal of Internal Korean Medicine / v.34, no.4, 2013 , pp. 339-348 More about this Journal
Abstract
Objectives : Danguieumja is a traditional medicinal prescription to treat skin disease. It was commonly used for the treatment of itching, chronic urticaria and atopic dermatitis in Korea by the addition or omission of several herbs. This study investigated the anti-inflammatory potential of Gagam-Danguieumja (GDE) water extract. Methods : We examined the effects of GDE on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) in a murine macrophage cell line, RAW 264.7 cells. Results : GDE inhibited production of NO in a dose dependent manner and also decreased the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2). As a possible molecular mechanism of anti-inflammatory effect increased phosphorylation of mitogen-activating protein kinases (MAPK) by LPS were blocked by GDE treatment. Conclusions : These results suggest that GDE has an anti-inflammatory therapeutic potential through the inhibition of MAPK phosphorylation, thereby decreasing the expression of pro-inflammatory genes.
Keywords
Gagam-Danguieumja; anti-inflammation; nitric oxide; inducible nitric oxide synthase; cyclooxygenase-2; mitogen-activating protein kinase;
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Times Cited By KSCI : 5  (Citation Analysis)
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