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The effect of Sagunja-tang on TNBS-induced Inflammatory Bowel Disease in Mouse  

Hong, Sang-Sun (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
Ryu, Bong-Ha (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
Yoon, Seong-Woo (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
Kim, Jin-Sung (3rd Dept. of Internal Medicine, College of Oriental Medicine, Kyung-Hee University)
Publication Information
The Journal of Internal Korean Medicine / v.31, no.4, 2010 , pp. 731-751 More about this Journal
Abstract
Objectives : The present study aimed to find out the effect of Sagunja-tang on the prevention and treatment of inflammatory bowel disease using mice with TNBS-induced inflammatory bowel disease. Methods : Mice with TNBS-induced inflammatory bowel disease were medicated with Sagunja-tang, and the weight changes, colon length, lipid peroxidation, and myeloperoxidase activity were observed. Levels of the inflammatory markers interleukin (IL)-$1{\beta}$ and cyclooxygenase-2 (COX-2), its transcription factor activation, phospho-NF-${\kappa}$B (pp65), in the colon by enzyme-linked immunosorbent assay and immunoblot analysis were also measured. Finally, the activation of fecal bacterial enzyme, ${\beta}$-glucuronidase and degradation activation of fecal glycosaminoglycan (GAG) and hyaluronic acid were observed. Results : We found that oral administration of Sagunja-tang inhibited TNBS-induced colon shortening and also inhibited myeloperoxidase activity in the colon of mice as well as IL-$1{\beta}$ and COX-2 expression. Sagunja-tang also inhibited TNBSinduced lipid peroxidation and pp65 activation in the colon of mice. In addition, Sagunja-tang inhibited ${\beta}$-glucuronidase activation and fecal hyaluronic acid degradation activation. Conclusions : It is supposed that Sagunja-tang has a potential therapeutic effect on inflammatory bowel disease through the inhibition of both NF-${\kappa}$B activation and lipid peroxidation, and the improvement of intestinal conditions.
Keywords
Sagunja-tang; Inflammatory Bowel Disease; 2,4,6-trinitrobenzene sulfonic acid(TNBS);
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