Browse > Article
http://dx.doi.org/10.5658/WOOD.2019.47.6.751

Acanthophysium sp. KMF001, a New Strain with High Cellulase Activity  

YOON, Sae-Min (Department of Forest Products and Biotechnology, Kookmin University)
PARK, So-Hyun (Department of Forest Products and Biotechnology, Kookmin University)
KIM, Tea-Jong (Department of Forest Products and Biotechnology, Kookmin University)
KIM, Young-Kyoon (Department of Forest Products and Biotechnology, Kookmin University)
KIM, Yeong-Suk (Department of Forest Products and Biotechnology, Kookmin University)
Publication Information
Journal of the Korean Wood Science and Technology / v.47, no.6, 2019 , pp. 751-760 More about this Journal
Abstract
Cellulase is an eco-friendly biocatalyst, and its demand is growing in many industrial applications such as food, textile, paper, and bioenergy. Strains with a high cellulase activities are the starting point for the economic production of cellulase. In a previous study, Acanthophysium sp. KMF001 with high cellulase production ability was selected among 54 wood-rotting fungi. In this study, we evaluated the cellulase productivity of Acanthophysium sp. KMF001 quantitatively and analyzed its taxonomic location using a genetic method. Acanthophysium sp. KMF001 showed high cellulase productivity similar to that of Acanthophysium bisporum and was much better than A. bisporum in specific enzyme activity. The 28S rRNA sequence of Acanthophysium sp. KMF001 was similar to that of Acanthophysium lividocaeruleum MB1825, with 98.40% homology. Phylogenetic analysis suggested that Acanthophysium sp. KMF001 is a new strain. In this study, we propose a new strain with high cellulase productivity.
Keywords
cellulase; Acanthophysium; new strain; 28S rRNA; phylogenetic;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Ali, S.R., Muthuvelayudham, R., Viruthagiri, T. 2013. Enhanced production of cellulase from tapioca stem using response surface methodology. Innovative Romanian Food Biotechnology 12(March): 40-51.
2 Biswas, A.K., Umeki, K., Yang, W., Blasiak, W. 2011. Change of pyrolysis characteristics and structure of woody biomass due to steam explosion pretreatment. Fuel Processing Technology 92(10): 1849-1854.   DOI
3 Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2): 248-254.   DOI
4 Castellanos, O.F., Sinitsyn, A.P., Vlasenko, E.Y. 1995. Comparative evaluation of hydrolytic efficiency toward microcrystalline cellulose of Penicillium and Trichoderma cellulases. Bioresource Technology 52(2): 119-124.   DOI
5 Delabona, P.d.S., Farinas, C.S., da Silva, M.R., Azzoni, S.F., Pradella, J.G.d.C. 2012. Use of a new Trichoderma harzianum strain isolated from the Amazon rainforest with pretreated sugar cane bagasse for on-site cellulase production. Bioresource Technology 107: 517-521.   DOI
6 Esterbauer, H., Steiner, W., Labudova, I., Hermann, A., Hayn, M. 1991. Production of Trichoderma cellulase in laboratory and pilot scale. Bioresource Technology 36(1): 51-65.   DOI
7 Fang, H., Zhao, C., Song, X.-Y. 2010. Optimization of enzymatic hydrolysis of steam-exploded corn stover by two approaches: Response surface methodology or using cellulase from mixed cultures of Trichoderma reeseiRUT-C30 and Aspergillus niger NL02. Bioresource Technology 101(11): 4111-4119.   DOI
8 Goldbeck, R., Ramos, M.M., Pereira, G.A.G., Maugeri-Filho, F. 2013. Cellulase production from a new strain Acremonium strictumisolated from the Brazilian biome using different substrates. Bioresource Technology 128: 797-803.   DOI
9 Gao, D., Haarmeyer, C., Balan, V., Whitehead, T.A., Dale, B.E., Chundawat, S.P.S. 2014. Lignin triggers irreversible cellulase loss during pretreated lignocellulosic biomass saccharification. Biotechnology for Biofuels 7(1): 175.   DOI
10 Gao, J., Weng, H., Zhu, D., Yuan, M., Guan, F., Xi, Y. 2008. Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. Bioresource Technology 99(16): 7623-7629.   DOI
11 Goyal, A., Ghosh, B., Eveleigh, D. 1991. Characteristics of fungal cellulases. Bioresource Technology 36(1): 37-50.   DOI
12 Haigler, C.H., Weimer, P.J. 1991. Biosynthesis and biodegradation of cellulose. Marcel Dekker New York.
13 Jamshidian, H., Shojaosadati, S.A., Vilaplana, F., Mousavi, S.M., Soudi, M.R. 2016. Characterization and optimization of schizophyllan production from date syrup. International Journal of Biological Macromolecules 92: 484-493.   DOI
14 Hong, J., Tamaki, H., Akiba, S., Yamamoto, K., Kumagai, H. 2001. Cloning of a gene encoding a highly stable endo-a-1, 4-glucanase from Aspergillus nigerand its expression in yeast. Journal of Bioscience and Bioengineering 92(5): 434-441.   DOI
15 Howard, R., Abotsi, E., Jansen van Rensburg, E. 2002. Lignocellulose biotechnology: Issues of bioconversion and enzyme production. African Journal of Biotechnology 2(12): 602-619.   DOI
16 Jorgensen, H., Eriksson, T., Börjesson, J., Tjerneld, F., Olsson, L. 2003. Purification and characterization of five cellulases and one xylanase from Penicillium brasilianum IBT 20888. Enzyme and Microbial Technology 32(7): 851-861.   DOI
17 Leathers, T.D., Sutivisedsak, N., Nunnally, M.S., Price, N.P.J., Stanley, A.M. 2015. Enzymatic modification of schizophyllan. Biotechnology Letters 37(3): 673-678.   DOI
18 Kim, J.Y., Yoon, S.M., Kim, Y.S. 2015a. Cellulase Activity of Symbiotic Bacteria from Snails, Achatina fulica. Journal of the Korean Wood Science and Technology 43(5): 628-640.   DOI
19 Kim, Y.S., Kim, T.J., Shin, K., Yoon, S.M. 2015b. Novel Acanthophysiumsp. KMF001 having high cellulase activity. US patent Application number: 14/930585.
20 Kurabi, A., Berlin, A., Gilkes, N., Kilburn, D., Bura, R., Robinson, J., Markov, A., Skomarovsky, A., Gusakov, A., Okunev, O., Sinitsyn, A., Gregg, D., Xie, D., Saddler, J. 2005. Enzymatic hydrolysis of steam-exploded and ethanol organosolv-pretreated douglas-fir by novel and commercial fungal cellulases. Applied Biochemistry and Biotechnology 121(1): 219-230.   DOI
21 Pandey, A., Webb, C., FERNANDES, M., Larroche, C. 2006. Enzyme Technology. Springer-Verlag New York Inc., New York.
22 Lee, B.-H., Kim, B.-K., Lee, Y.-J., Chung, C.-H., Lee, J.-W. 2010. Industrial scale of optimization for the production of carboxymethylcellulase from rice bran by a marine bacterium, Bacillus subtilis subsp. subtilis A-53. Enzyme and Microbial Technology 46(1): 38-42.   DOI
23 Li, Y.-H., Ding, M., Wang, J., Xu, G.-j., Zhao, F. 2006. A novel thermoacidophilic endoglucanase, Ba- EGA, from a new cellulose-degrading bacterium, Bacillus sp. AC-1. Applied Microbiology and Biotechnology 70(4): 430-436.   DOI
24 Nelson, N. 1944. A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153(2): 375-380.   DOI
25 Rosgaard, L., Pedersen, S., Cherry, J.R., Harris, P., Meyer, A.S. 2006. Efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose. Biotechnology Progress 22(2): 493-498.   DOI
26 Tamura, K., Dudley, J., Nei, M., Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24(8): 1596-1599.   DOI
27 Schulein, M. 1988. Cellulases of Trichoderma reesei, Methods in Enzymology (vol. 160. pp. 234-242), Academic Press.
28 Shin, K., Yoon, S.-M., Kim, J.H., Kim, Y.-K., Kim, T.-J., Kim, Y.-S. 2016. Biopolishing of cotton fabric using crude cellulases from Acanthophysium sp. KMF001. Journal of the Korean Wood Science and Technology 44(3): 381-388.   DOI
29 Smits, J.P., Rinzema, A., Tramper, J., Sonsbeek, H.M.V., Knol, W. 1996. Solid-state fermentation of wheat bran by Trichoderma reesei QM9414: substrate composition changes, C balance, enzyme production, growth and kinetics. Applied Microbiology and Biotechnology 46(5): 489-496.   DOI
30 Sutivisedsak, N., Leathers, T.D., Bischoff, K.M., Nunnally, M.S., Peterson, S.W. 2013. Novel sources of a-glucanase for the enzymatic degradation of schizophyllan. Enzyme and Microbial Technology 52(3): 203-210.   DOI
31 Yoon, S.-M., Kim, Y.-S., Kim, Y.-K., Kim, T.-J. 2018. A novel endo-a-1,4-xylanase from Acanthophysium sp. KMF001, a wood rotting fungus. Journal of the Korean Wood Science and Technology 46(6): 670-680.   DOI
32 Zhang, H., Sang, Q., Zhang, W. 2012. Statistical optimization of cellulases production by Aspergillus nigerHQ-1 in solid-state fermentation and partial enzymatic characterization of cellulases on hydrolyzing chitosan. Annals of Microbiology 62(2): 629-645.   DOI
33 Zhu, J.Y., Pan, X.J., Wang, G.S., Gleisner, R. 2009. Sulfite pretreatment (SPORL) for robust enzymatic saccharification of spruce and red pine. Bioresource Technology 100(8): 2411-2418.   DOI