Browse > Article
http://dx.doi.org/10.5658/WOOD.2016.44.1.124

Biomodification of Ethanol Organolsolv Lignin by Abortiporus biennis and Its Structural Change by Addition of Reducing Agent  

Hong, Chang-Young (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University)
Park, Se-Yeong (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University)
Kim, Seon-Hong (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University)
Lee, Su-Yeon (Department of Forest Products Engineering, Korea Forest Research Institute)
Ryu, Sun-Hwa (Department of Forest Products Engineering, Korea Forest Research Institute)
Choi, In-Gyu (Department of Forest Sciences, College of Agriculture and Life Sciences, Seoul National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.44, no.1, 2016 , pp. 124-134 More about this Journal
Abstract
The main goal of this study was to investigate biomodification mechanism of lignin by white rot fungus, Abortiporus biennis, and to depolymerize ethanol organosolv lignin for industrial application. In nitrogen-limited culture, A. biennis polymerized mainly lignin showing a rapid increase of molecular weight and structural changes depending on incubation days. At the initial incubation days, cleavage of ether bonds increased phenolic OH content, while the results were contrary in of the later part of the culture. Based on these results, ascorbic acid as a reducing agent was used to induce depolymerization of lignin during cultivation with white rot fungus. As a result, the degree of increase of average molecular weight of lignin was significantly declined when compared with those of the ascorbic acid free-experiment, although the molecular weight of fungus treated sample slightly increased than that of control. Furthermore, lignin derived oligomers in culture medium were depolymerized with the addition of ascorbic acid, showing that the average molecular weight was 381 Da, and phenolic OH content was 38.63%. These depolymerized lignin oligomers were considered to be applicable for industrial utilization of lignin. In conclusion, A. biennis led to the polymerization of lignin during biomodification period. The addition of ascorbic acid had a positive effect on the depolymerization and increase of phenolic OH content of lignin oligomers in medium.
Keywords
biomodification; depolymerization; lignin oligomers; white rot fungus; Abortiporus biennis;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Baucher, M., Monties, B., Montagu, M.V., Boerjan, W. 1998. Biosynthesis and genetic engineering of lignin. Critical reviews in plant sciences 17(2): 125-197.   DOI
2 Eriksson, K.-E.L., Blanchette, R.A., Ander, P. 1990. Microbial and enzymatic degradation of wood and wood components. Springer-Verlag.
3 Gouveia, S., Fernandez-Costas, C., Sanroman, M., Moldes, D. 2013. Polymerisation of Kraft lignin from black liquors by laccase from Myceliophthora thermophila: Effect of operational conditions and black liquor origin. Bioresource technology 131: 288-294.   DOI
4 Huttermann, A., Mai, C., Kharazipour, A. 2001. Modification of lignin for the production of new compounded materials. Applied microbiology and biotechnology 55(4): 387-394.   DOI
5 Haemmerli, S.D., Leisola, M.S., Fiechter, A. 1986. Polymerisation of lignins by ligninases from Phanerochaete chrysosporium. FEMS microbiology letters 35(1): 33-36.   DOI
6 Higuchi, T. 1986. Catabolic Pathways and Role of Ligninases for the Degradation of Lignin Substructure Models by White-Rot Fungi. Wood research: bulletin of the Wood Research Institute Kyoto University 73: 58-81.
7 Higuchi, T. 1990. Lignin biochemistry: biosynthesis and biodegradation. Wood Science and Technology 24(1): 23-63.   DOI
8 Himmel, M.E. 2009. Biomass recalcitrance: deconstructing the plant cell wall for bioenergy. Wiley-Blackwell.
9 Hong, C.Y., Kim, H.Y., Jang, S.K., Choi, I.G. 2013. Screening of outstanding white rot fungi for biodegradation of organosolv lignin by decolorization of Remazol Brilliant Blue R and ligninolytic enzymes systems. Journal of the Korean Wood Science and Technology 41(1): 19-32.   DOI
10 Iwahara, K., Honda, Y., Watanabe, T., Kuwahara, M. 2000. Polymerization of guaiacol by lignin-degrading manganese peroxidase from Bjerkandera adusta in aqueous organic solvents. Applied microbiology and biotechnology 54(1): 104-111.   DOI
11 Johnson, C.W., Beckham, G.T. 2015. Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin. Metabolic engineering 28: 240-247.   DOI
12 Kawai, S., Asukai, M., Ohya, N., Okita, K., Ito, T., Ohashi, H. 1999. Degradation of a non-phenolic ${\beta}$-O-4 substructure and of polymeric lignin model compounds by laccase of Coriolus versicolor in the presence of 1-hydroxybenzotriazole. FEMS microbiology letters 170(1): 51-57.   DOI
13 Kawai, S., Umezawa, T., Higuchi, T. 1988. Degradation mechanisms of phenolic ${\beta}$-1 lignin substructure model compounds by laccase of Coriolus versicolor. Archives of biochemistry and biophysics 262(1): 99-110.   DOI
14 Kersten, P.J., Tien, M., Kalyanaraman, B., Kirk, T.K. 1985. The ligninase of Phanerochaete chrysosporium generates cation radicals from methoxybenzenes. Journal of Biological Chemistry 260(5): 2609-2612.
15 Linger, J.G., Vardon, D.R., Guarnieri, M.T., Karp, E.M., Hunsinger, G.B., Franden, M.A., Johnson, C.W., Chupka, G., Strathmann, T.J., Pienkos, P.T. 2014. Lignin valorization through integrated biological funneling and chemical catalysis. Proceedings of the National Academy of Sciences 111(33): 12013-12018.
16 Kinne, M., Poraj-Kobielska, M., Ralph, S.A., Ullrich, R., Hofrichter, M., Hammel, K.E. 2009. Oxidative cleavage of diverse ethers by an extracellular fungal peroxygenase. Journal of Biological Chemistry 284(43): 29343-29349.   DOI
17 Kinne, M., Poraj-Kobielska, M., Ullrich, R., Nousiainen, P., Sipila, J., Scheibner, K., Hammel, K.E., Hofrichter, M. 2011. Oxidative cleavage of non-phenolic${\beta}$-O-4 lignin model dimers by an extracellular aromatic peroxygenase. Holzforschung 65(5): 673-679.   DOI
18 Kudanga, T., Nyanhongo, G.S., Guebitz, G.M., Burton, S. 2011. Potential applications of laccase- mediated coupling and grafting reactions: a review. Enzyme and microbial technology 48(3): 195-208.   DOI
19 Liu, J., Ye, L., Weiping, Y. 1999. Copolymerization of lignin with cresol catalysed by peroxidase in reversed micellar systems. Electronic Journal of Biotechnology 2(2): 7-8.
20 Lora, J.H., Glasser, W.G. 2002. Recent industrial applications of lignin: a sustainable alternative to nonrenewable materials. Journal of Polymers and the Environment 10(1-2): 39-48.   DOI
21 Martinez, A.T., Speranza, M., Ruiz-Duenas, F.J., Ferreira, P., Camarero, S., Guillen, F., Martinez, M.J., Gutierrez, A., del Rio, J.C. 2010. Biodegradation of lignocellulosics: microbial, chemical, and enzymatic aspects of the fungal attack of lignin. International Microbiology 8(3): 195-204.
22 Meister, J.J. 2002. Modification of Lignin. Journal of Macromolecular Science, Part C: Polymer Reviews 42(2): 235-289.   DOI
23 Pointing, S. 2001. Feasibility of bioremediation by white-rot fungi. Applied Microbiology and Biotechnology 57(1-2): 20-33.   DOI
24 Morohoshi, N., Haraguchi, T., Wariishi, H., Muraiso, C., Nagai, T. 1987. Degradation of lignin by the extracellular enzymes of Coriolus versicolor, 4: Properties of three laccase fractions fractionated from the extracellular enzymes. Journal of the Japan Wood Research Society (Japan).
25 Nugroho Prasetyo, E., Kudanga, T., Ostergaard, L., Rencoret, J., Gutierrez, A., del Rio, J.C., Ignacio Santos, J., Nieto, L., Jimenez-Barbero, J., Martínez, A.T. 2010. Polymerization of lignosulfonates by the laccase-HBT (1-hydroxybenzotriazole) system improves dispersibility. Bioresource technology 101(14): 5054-5062.   DOI
26 Onnerud, H., Zhang, L., Gellerstedt, G., Henriksson, G. 2002. Polymerization of Monolignols by Redox Shuttle-Mediated Enzymatic Oxidation A New Model in Lignin Biosynthesis I. The Plant Cell 14(8): 1953-1962.   DOI
27 Pollegioni, L., Tonin, F., Rosini, E. 2015. Lignin-degrading enzymes. FEBS Journal 282(7): 1190-1213.   DOI
28 Sahoo, S., Seydibeyoglu, M., Mohanty, A., Misra, M. 2011. Characterization of industrial lignins for their utilization in future value added applications. Biomass and bioenergy 35(10): 4230-4237.   DOI
29 Schmidt, O. 2006. Wood and tree fungi. Springer.
30 Sena-Martins, G., Almeida-Vara, E., Duarte, J. 2008. Eco-friendly new products from enzymatically modified industrial lignins. Industrial crops and products 27(2): 189-195.   DOI
31 Singh, D., Zeng, J., Laskar, D.D., Deobald, L., Hiscox, W.C., Chen, S. 2011. Investigation of wheat straw biodegradation by Phanerochaete chrysosporium. Biomass and Bioenergy 35(3): 1030-1040.   DOI
32 Youn, H.D., Hah, Y.C., Kang, S.O. 1995. Role of laccase in lignin degradation by white-rot fungi. FEMS Microbiology Letters 132(3): 183-188.   DOI
33 Stewart, D. 2008. Lignin as a base material for materials applications: Chemistry, application and economics. Industrial Crops and Products 27(2): 202-207.   DOI
34 Tien, M. 1987. Properties of ligninase from Phanerochaete chrysosporium and their possible applications. Critical reviews in microbiology 15(2): 141-168.   DOI
35 Tien, M., Kirk, T.K. 1983. Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science (Washington) 221(4611): 661-662.   DOI