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Biodegradation of Phthalic acid by White rot Fungus, Polyporus brumalis  

Lee, Soo-Min (Dept. of Forest Science, College of Agriculture & Life Sciences, Seoul National University)
Park, Ki-Ryung (Dept. of Forest Science, College of Agriculture & Life Sciences, Seoul National University)
Lee, Sung-Suk (Dept. of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Kim, Myung-Kil (Dept. of Wood Chemistry & Microbiology, Korea Forest Research Institute)
Choi, In-Gyu (Dept. of Forest Science, College of Agriculture & Life Sciences, Seoul National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.33, no.1, 2005 , pp. 48-57 More about this Journal
Abstract
Phthalate esters are known as plasticizers and some of them suspected as endocrine disrupting chemicals. In this study, in order to identify the mechanism of phthalate esters degradation by white rot fungus, phthalic acid, which is major metabolite in the biodegradation of phthalate esters, was used. Phthalic acid 50 ppm was treated in culture medium with Polyporus brumalis. The availability of ABTS oxidation was different from control and phthalic acid treated group after 4 days of incubation. The activity was gradually increased in control group, but not in phthalic acid treated group. Especially, esterase activity of control group was maximized at 10 days of incubation, and then decreased while the activity of phthalic acid treated group was increased. Glucose was used as a carbon source, and the difference of glucose consumption by control and phthalic acid treated group was not significant. However, after 6 days of incubation the residual glucose in culture medium was rapidly decreased. The consumption rate of phthalic acid treated group was lower than control. These results might indicate that the absorption of phthalic acid in culture medium was occurred by mycelium and metabolized through some pathways as that of glucose was. To clearify the chemical modification of phthalic acid in culture medium, phthalic acid was reacted under in vitro condition which mycelium was excluded. The metabolites were analyzed by GC/MS. The results showed that phthalic acid was converted to phthalic acid anhydride by the extracellular enzymes of P. brumalis.
Keywords
phthalic acid; biodegradation; white rot fungus; Polyporus brumalis; lignin degrading enzymes;
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1 최인규, 안세희. 1998. 목질 분해균에 의한 penta- chlorophenol의 미생물 분해, 목재공학 26(3): 53-62
2 http://www.atsdr.cdc.gov/clist-supportdoc.html
3 Kim, Y. H., J. W. Lee, J. Y. Ahn, M. B. Gu, and S. H. Moon. 2002. Enhanced degradation of an endocrine- disrupting chemical, butyl benzyl phthalate by Fusarium oxysporum f. sp. pisi cutinase. Appl. Environ. Microbiol. 68: 4684-4688   DOI   ScienceOn
4 Bradford, M. M. 1976. A rapid and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254
5 Fett, W. F., H. C. Gerad, R. A. Moreau, S. F. Osman, and L. E. Jones. 1992. Cutinase production by Streptomyces spp. Curr. Microbiol. 25: 165-171   DOI
6 Lobos, J. H., T. K. Leib, and T. M. Su. 1992. Biodegradation of bisphenol A and other bisphenols by a gram-negative aerobic bacterium, Appl. Environ. Microbiol. 58: 1823-1831   PUBMED
7 최인규, 이재원, 최돈하. 2002. Monochlorophenol 의 목질 분해균에 의한 분해 특성. 한국환경농학회지 21(4): 261-268
8 Ronen, Z. and A. Abeliovich. 2000. Anaerobicaerobic process for microbial degradation of tetrabromophenol A. Appl, Environ. Microbiol. 66:2372-2377   DOI   ScienceOn
9 안세희, 최인규. 1998. 목질 분해균에 의한 4,5,6-tri-guaiacol의 미생물 분해. 목재공학 26(3): 63-72
10 Chatterjee, S. and T. K. Dutta. 2003. Metabolism of butyl benzyl phthalate by Gordonia sp. strain MTCC 4818. Biochem. Biophysics. Res. Comm. 309: 36-43   DOI   ScienceOn
11 Hammel, K. E., B. Kalyanaraman, and T. K. Kirk. 1986. Oxidation of polycyclic aromatic hydrocarbons and dibenzo[p]dioxins by Phanerochaete chrysosporium ligninase. J. Biol. Chem. 261: 16948-16952
12 Tien, M. and T. K. Kirk. 1984. Lignin-degrading enzyme from Pbanerocbaete cbrysosporium: purification, characterization, and catalytic properties of a unique $H_20_2$-requiring oxygenase, Pro. Nat'l Acad. Sci. USA 81: 2280-2284
13 Gutierrez A, J. C. del Rio, M. J. Martinez-Inigo, M. .J. Martinez, and A. T. Martinez. 2002. Production of new unsaturated lipids during wood decay by ligninolytic basidiomycetes. Appl. Environ. Microbiol. 68: 1344-1350   DOI   ScienceOn
14 Furusawa, S., S. Nakai, and M. Hosomi. 2000. Microbial degradation of 4-Nonylphenol. J. Jpn Soc. Water Environ. 23: 243-245   DOI   ScienceOn
15 Sato A, H. Justica, .J. W. Wray, and C. Sonnenschein. 1991. p-Nonylphenol and estrogenic xenobiotic released from 'modified' polystyrene, Environ. Health Perspect. 92: 167-173   DOI   PUBMED
16 Lee, S. M., B. K. Koo, M. K. Kim, D. H. Choi, E. J. Hong, E. B. Jeung, and I. G. Choi. 2004. Biodegradation of dibutylphthalate by white rot fungi and evaluation on its estrogenic activity. Enzym. Microbial Technol. 35: 417-423   DOI   ScienceOn
17 이수민, 구본욱, 이재윈, 최돈하, 정의배, 최인규. 2004. 옥틸페놀 (4-t-octylphenol)의 Basidioradulum molare와 Schizopora paradoxa에 의한 분해 및 에스트로겐 성 저감 효과. 목재공학. 32: 27-35
18 Miller G. L. 1958. Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Biochem. 1: 426-506
19 Staples, C. A., D. R. Peterson, T. F. Parkerton, and W. J. Adams. 1997. The Environmental Fate of Phthalate Esters: a literature review. Chemosphere 35: 667-749   DOI   ScienceOn
20 Glenn, J. K., L. Akileswaran, and M. H. Gold. 1986. Mn(II) oxidation is the principal function of the extracellular Mn-perxoidase from Phanerochaete chrysosporium. Arch. Biochem. Biophysics. 251:688-696   DOI   ScienceOn