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http://dx.doi.org/10.22889/KJP.2022.53.4.192

Comparison of Bioactivities from Centella asiatica Cultivated in Smart Farm and Field  

Jin Hong, Park (Rokya Co., Ltd.)
Da Hee, Lee (Rokya Co., Ltd.)
Seong Min, Jo (Rokya Co., Ltd.)
Jeong Hwan, Choi (Rokya Co., Ltd.)
Nam Jun, Kim (Rokya Co., Ltd.)
Min Su, Kim (Rokya Co., Ltd.)
Youngmin, Park (Rokya Co., Ltd.)
Kiman, Lee (Rokya Co., Ltd.)
Publication Information
Korean Journal of Pharmacognosy / v.53, no.4, 2022 , pp. 192-201 More about this Journal
Abstract
This study aimed to compare bioactivities of Centella asiatica (CA) cultivated in smart farms and fields. Component analysis, cell viability, anti-inflammatory activity, neuroprotection activity, and antioxidant activity were examined with 70% ethanol extracts of CA cultivated in smart farm (SEE) and field (FEE), respectively. Asiaticoside was analyzed by high performance liquid chromatography (HPLC) and as a result, SEE had more asiaticoside content than FEE. After treatment of RAW 264.7 cells with SEE and FEE, there was no cytotoxicity within the treated concentrations. SEE and FEE showed nitric oxide (NO), tumor necrosis factor (TNF)-α, and interleukin (IL)-6 inhibitory activities in a dose-dependent manner in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Moreover, SEE inhibited more NO, TNF-α, and IL-6 production levels than FEE. SEE and FEE reversed the H2O2-induced SH-SY5Y cell death. Especially, SEE was more effective in changing the H2O2-induced SH-SY5Y cell death than FEE. The antioxidant activity was confirmed by various methods such as total phenol content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and superoxide dismutase (SOD). As a result, SEE showed the most potent antioxidant activities about TPC, DPPH, and SOD methods. This study suggested that SEE has higher bioactivities such as effect of anti-inflammation, neuroprotection, and antioxidation than FEE.
Keywords
Centella asiatica; Anti-inflammatory; Antioxidant; Asiaticoside; Neuroprotection;
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