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http://dx.doi.org/10.22889/KJP.2022.53.2.102

The Effect of Vitis labruscana B. Leaves Ethanol Extract on the Expression of Amyloid Precursor Protein in Neuroblastoma Cells and on the Acetylcholinesterase Activity  

Choi, Ha Yeon (College of Food Science and Nutrition, Woosuk University)
Kim, Ju Eun (College of Pharmacy, Woosuk University)
Ma, Sang Yong (College of Food Science and Nutrition, Woosuk University)
Cho, Hyung Kwon (Hanpoong Pharm. Co., LTD)
Kim, Dae Sung (Hanpoong Pharm. Co., LTD)
Leem, Jae Yoon (College of Pharmacy, Woosuk University)
Publication Information
Korean Journal of Pharmacognosy / v.53, no.2, 2022 , pp. 102-110 More about this Journal
Abstract
Alzheimer's disease (AD) is the most common form of dementia, and the accumulation of β-amyloid (Aβ) in the brain triggers AD, followed by hyperphosphorylation of tau protein, neurofibrillary tangles, and synapses loss, neuronal cell death, and cognitive decline occur in a chain. In APPswe neuronal cell line, 50 ㎍/ml of Campbell early (Vitis labruscana B.) leaves 50% ethanol extract (VLL) treatment inhibited the secretion of Aβ1-42 by about 63% and the secretion of Aβ1-40 by about 50%. VLL did not target the enzymatic activity of the amyloidogenic pathway and decreased the protein expression of APP. As a result of RT-qPCR (Reverse transcription-quantitative real-time PCR) of the APPswe cell line treated with VLL, it is thought that the protein expression of APP was reduced by inhibiting the transcription process of the APP gene. In addition, VLL inhibited acetylcholinesterase (AChE) enzyme activity in vitro by 27.6% and 54.7%, respectively, at 50 and 100 ㎍/ml concentrations. We found that VLL inhibited the production of Aβ, a dementia-inducing substance, by suppressing the transcription of the APP gene, and that VLL inhibited AChE activity. We suggest that VLL has the potential as a natural drug material that modulates the alleviation of dementia symptoms.
Keywords
Alzheimer's disease; ${\beta}-amyloid$; Amyloid precursor protein; Acetylcholinesterase; Vitis labruscana B;
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