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http://dx.doi.org/10.22889/KJP.2021.52.3.186

HPLC/DAD Method Validation of 6-Hydroxyluteolin 7-O-Glucoside Analysis from Salvia plebeia  

Lee, Hak-Dong (Department of Plant Science and Technology, Chung-Ang University)
Paje, Leo Adrianne (Department of Plant Science and Technology, Chung-Ang University)
Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University)
Kim, Juree (Department of Plant Science and Technology, Chung-Ang University)
Yu, A Ram (Department of Plant Science and Technology, Chung-Ang University)
Bae, Min-Jung (Technical Assistance Department, The Food Industry Promotional Agency of Korea)
Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
Publication Information
Korean Journal of Pharmacognosy / v.52, no.3, 2021 , pp. 186-191 More about this Journal
Abstract
We developed a method to identify and quantify 6-hydroxyluteolin 7-O-glucoside in the powder of Salvia plebeia (PS) using high-performance liquid chromatography coupled with diode array detector (HPLC/DAD) and equipped with reverse-phase INNO C18 column. The analytical method was optimized and validated using novel parameters. The obtained values for the limits of detection and quantification were 3.60 and 10.90 ㎍/mL, respectively. Calibration curve showed good linearity in the concentration range tested (0.00625-0.1 mg/mL, r2 = 1.0000), high accuracy (96.2-101.4%), and precision values (RSD ≤ 0.27%). Our analysis support the use of our method for accurately identifying and quantifying 6-hydroxyluteolin 7-O-glucoside from PS in routine analyses and large-scale extraction processes for content determination.
Keywords
Salvia plebeia; 6-Hydroxyluteolin 7-O-glucoside; Method validation; HPLC/DAD;
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