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http://dx.doi.org/10.22889/KJP.2021.52.3.163

Antioxidant Activity of Ethyl acetate Fraction of the Guzeunggupo-procossed Platycodon grandiflorum A. De Candolle roots in Caenorhabditis elegans  

Kwon, Kang Mu (College of Pharmacy, Woosuk University)
Kim, Jun Hyeong (Department of Food and Biotechnology, Woosuk University)
Yang, Jae Heon (Ainara)
Ki, Byeolhui (Ainara)
Hwang, In Hyun (College of Pharmacy, Woosuk University)
Kim, Dae Keun (College of Pharmacy, Woosuk University)
Publication Information
Korean Journal of Pharmacognosy / v.52, no.3, 2021 , pp. 163-169 More about this Journal
Abstract
Using the Caenorhabditis elegans model system, the antioxidant activity of methanol extract of the guzeunggupoprocessed Liriope platyphylla F. T. Wang (Liliaceae) tuber was calculated. Between the methanol extracts of guzeunggupo-processed and non-processed L. platyphylla tuber, the processed L. platyphylla tuber showed higher DPPH radical scavenging effect than the non-processed one. The ethyl acetate soluble fraction of the methanol extract of the guzeunggupo-processed L. platyphylla tuber showed the best DPPH radical scavenging activity. The ethyl acetate fraction of the processed sample was measured for the activities of superoxide dismutase (SOD), catalase, and oxidative stress tolerance by using C. elegans along with reactive oxygen species level. In addition, to verify the regulation of the stress response gene is responsible for the increased stress tolerance of C. elegans treated by the ethyl acetate fraction of the processed sample, SOD-3 expression was measured using a transgenic strain (CF1553). Consequently, the ethyl acetate fraction of the processed sample, increased SOD and catalase activities, and decreased ROS accumulation in a dose-dependent manner. Furthermore, the ethyl acetate fraction of the processed sample-treated CF1553 worm showed higher SOD-3::GFP intensity than the control worm.
Keywords
Guzeugupo-processed Liriope platyphylla; Caenorhabditis elegans; Antioxidant activity; SOD; Catalase;
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1 Baek, N.-I., Cho, S.-J., Bang, M.-H., Lee, I., Park, C., Kim, M., Kim K. and Sung, J. (1998) Cytotoxicity of steroid-saponins from the tuber of Liriope platyphylla W. T. Appl. Biol. Chem. 41: 390-394.
2 Yang, S. and Lian, G. (2020) ROS and diseases: role in metabolism and energy supply. Mol. Cell Biochem. 467: 1-12.   DOI
3 Brenner, S. (1974) The genetics of Caenorhabditis elegans. Genetics 77: 71-94.   DOI
4 Kim, H. N., Seo, H. W., Kim, B. S., Lim H. J., Lee, H, N., Park, J. S., Yoon, Y. J., Oh, J. W., Oh, M. J., Kwon, J., Oh, C. H., Cha, D. S. and Jeon, H. (2015) Lindera obtusiloba extends lifespan of Caenorhabditis elegans. Nat. Prod. Sci. 21: 128-133.
5 Luo, S., Jiang, X., Jia, L., Tan, C.,Li, M., Yang, Q., Du, Y. and Ding, C. (2019) In vivo and in vitro antioxidant activities of methanol extracts from olive leaves on Caenorhabditis elegans. Molecules 704: 1-14.
6 Gao, G. Y., Ma, J., Lu, P., Jiang, X. and Chang, C. (2018) Ophiopogonin B induces the autophagy and apoptosis of colon cancer cells by activating JNK/c-Jun signaling pathway. Biomed. Pharmacother. 108: 1208-1215.   DOI
7 Moniruzzaman, M., Ghosal, I., Das, D. and Chakraborty, S. B. (2018) Melatonin ameliorates H2O2-induced oxidative stress through modulation of Erk/Akt/NFkB pathway. Biol. Res. 51: 17.   DOI
8 Eftekhari. A., Dizaj, S. M., Chodari, L., Sunar ,S., Hasanzadeh, A., Ahmadian. E. and Hasanzadeh. M. (2018) The promising future of nano-antioxidant therapy against environmental pollutants induced-toxicities. Biomed. Pharmacother. 103: 1018-1027.   DOI
9 Scialo, F., Fernandez-Ayala, D. J. and Sanz, A. (2017) Role of mitochondrial reverse electron transport in ROS signaling: Potential roles in health and disease. Front. Physiol. 8: 428.   DOI
10 Kim, I., Lee, J. and Jeong, Y. (2018) Antioxidant activities of Rumex crispus L. root extracts and fractions. J. Korean Soc. Food Sci. Nutr. 47: 1234-1241.   DOI
11 Kang, M.-J., Kim, D.-W., Kang, J.-R. and Shin, J.-H. (2018) Effect of roasting time on physicochemical characteristics and antioxidant activity of hot water extracts of dried Liriope platyphylla. Korean J. Food Preserv. 25: 246-254.   DOI
12 Yoshida, T., Mori, K., Hatano, T., Okumura, T., Uehara, I., Komagoe, K., Fujita, Y. and Okuda, T. (1989) Studies on inhibition mechanism of autooxidation by tannins and flavonoids. V: radical scavenging effects of tannins and related polyphenols on 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37: 1919-1921.   DOI
13 Aebi, H. (1984) Catalase in vitro. Methods Enzymol. 105: 121-126.   DOI
14 Mekheimer, R. A., Sayed, A. A. and Ahmed, E. A. (2012) Novel 1,2,4-triazolo[1,5-a]pyridines and their fused ring systems attenuate oxidative stress and prolong lifespan of Caenorhabiditis elegans. J. Med. Chem. 55: 4169-4177.   DOI
15 Alugoju. P., VKD, K. S. and Periyasamy, L. (2018) Effect of short-term quercetin, caloric restriction and combined treatment on age-related oxidative stress markers in the rat cerebral cortex. CNS Neurol Disord. Drug Targets 17: 119-131.   DOI
16 Kobet, R. A., Pan, X., Zhang, B., Pak, S. C., Asch, A. S. and Lee, M.-H. (2014) Caenorhabditis elegans: A model system for anti-cancer drug discovery and therapeutic target identification. Biomol. Ther. 22: 371-383.   DOI
17 Mekheimer, R. A., Sayed, A. A. and Ahmed, E. A. (2012) Novel 1,2,4-triazolo[1,5-a]pyridines and their fused ring systems attenuate oxidative stress and prolong lifespan of Caenorhabditis elegans. J. Med. Chem. 55: 4169-4177.   DOI
18 Stefanatos, R. and Sanz, A. (2018) The role of mitochondrial ROS in the aging brain. FEBS Lett. 592: 743-758.   DOI
19 Zhu, X., Wang, K. and Chen, Y. (2020) Ophiopogonin D suppresses TGF-beta1-mediated metastatic behavior of MDAMB-231 breast carcinoma cells via regulating ITGB1/FAK/Src/AKT/beta-catenin/MMP-9 signaling axis. Toxicol. In Vitro 69: 104973.   DOI
20 Li, N., Zhang, J. Y., Zeng, K. W., Zhang,. L., Che, Y. Y., Tu, P. F. (2012) Anti-inflammatory homoisoflavonoids from the tuberous roots of Ophiopogon japonicus. Fitoterapia 83: 1042-1045.   DOI
21 Hes, M., Dziedzic, K., Gorecka, D., Jedrusek-Golinska, A. and Gujska, E. (2019) Aloe vera (L.) Webb.: Natural sources of antioxidants - A review. Plant Foods Hum. Nutr. 74: 255-265.   DOI