Browse > Article
http://dx.doi.org/10.22889/KJP.2021.52.2.84

The Melanin Synthesis Inhibitory Effect of Aspergillus fumigatus Fermented Extract  

Song, Tae-Yang (Seoulin Bioscience Co., Ltd, KOREA BIO PARK)
Kim, Chang Won (Seoulin Bioscience Co., Ltd, KOREA BIO PARK)
Kang, Mi Ok (Seoulin Bioscience Co., Ltd, KOREA BIO PARK)
Gal, Sang Wan (Department of Microbiological Engineering, Jinju National University)
Hwang, Eul Moon (Seoulin Bioscience Co., Ltd, KOREA BIO PARK)
Publication Information
Korean Journal of Pharmacognosy / v.52, no.2, 2021 , pp. 84-91 More about this Journal
Abstract
This study was carried out to investigate the melanin synthesis inhibitory effect of Aspergillus fumigatus fermented extract. In this study, we revealed the effects of A. fumigatus fermented extract on melanin contents, mushroom tyrosinase activity, and expression levels of mRNA and proteins of melanogenesis-related gene in B16F10 melanoma cells. A. fumigatus fermented extract inhibited both melanin contents and tyrosinase activity. In addition, the expression level of mRNA or proteins of melanogenesis was down-regulated in the A. fumigatus fermented extract treated B16F10 cells with dose-dependent manner. Moreover, when the clinical test was conducted, it was confirmed that the use of the fermented extract of A. fumigatus for 8 weeks improved skin brightness 1.586 times brighter and skin melanin 1.331 times better compared to the control product. Taken together, our results suggest that A. fumigatus fermented extract has melanogenesis inhibitory effect and whitening activity, thus it showed the possibility for using as a functional whitening cosmetic resource.
Keywords
Aspergillus fumigatus; Melanin; Tyrosinase; Cosmetic resource;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Kang, M. K., Lee, Y. E., Woo, W. H. and Mun, Y. J. (2014) Commelina communis Ledeb inhibits melanin synthesis in alpha-MSH-stimulated B16F10 Cells. J. Physiol. & Pathol. Korean Med. 28: 506-511.
2 Tsao, Y. T., Huang, Y. F., Kuo, C. Y., Lin, Y. C., Chiang, W. C., Wang, W. K., Hsu, C. W. and Lee, C. H. (2016) Hinokitiol Inhibits melanogenesis via AKT/mTOR signaling in B16F10 mouse melanoma cells. Int. J. Mol. Sci.17: 248.   DOI
3 Wu, P. Y., You, Y. J., Liu, Y. J., Hou, C. W., Wu, C. S., Wen, K. C., Lin, C. Y. and Chiang, H. M. (2018) Sesamol Inhibited melanogenesis by regulating melanin-related signal transduction in B16F10 cells. Int. J. Mol. Sci. 19:1108.   DOI
4 Saeedi, M., Eslamifar, M. and Khezri, K. (2019) Kojic acid applications in cosmetic and pharmaceutical preparations. Biomed. Pharmacother. 110: 582-593.   DOI
5 Chang, C. T., Chang, W. L., Hsu, J. C. and Shih, Y. (2013) Chemical composition and tyrosinase inhibitory activity of Cinnamomum cassia essential oil. Bota. Stud. 54: 10-17.   DOI
6 Latge, J. P. and Chamilos, G. (2020) Aspergillus fumigatus and Aspergillosis in 2019. Clin. Microbiol. Rev. 33: e00140-18.
7 Luther, J. P. and Lipke, H. (1980) Degradation of melanin by Aspergillus fumigatus. Appl. Environ. Microbiol. 40: 145-155.   DOI
8 Inoue, Y., Hasegawa, S., Yamada, T., Date, Y., Mizutani, H., Nakata, H., Matsunaga, K. and Akamatsu, H. (2013) Analysis of the effects of hydroquinone and arbutin on the differentiation of melanocytes. Biol. Pharm. Bull. 36: 1722-1730.   DOI
9 Lim, Y. J., Lee, E. H., Kang, T. H., Ha, S. K., Oh, M. S., Kim, S. M., Yoon, T. J., Kang, C., Park, J. H. and Kim, S. Y. (2009) Inhibitory effects of arbutin on melanin biosynthesis of α-melanocyte stimulating hormone-induced hyperpigmentation in cultured brownish guinea pig skin tissues. Arch. Pharm. Res. 32: 367-373.   DOI
10 Abdel-Malek, Z., Swope, V. B., Suzuki, I., Akcali, C., Harriger, M. D., Boyce, S. T., Urabe, K. and Hearing, V. J. (1995) Mitogenic and melanogenic stimulation of normal human melanocytes by melanotropic peptides. Proc. Natl. Acad. Sci. USA. 92: 1789-1793.   DOI
11 Serre, C., Busuttil, V., and Botto, J. M. (2018) Intrinsic and extrinsic regulation of human skin melanogenesis and pigmentation. Int. J. Cosmet. Sci. 40: 328-347.   DOI
12 Maranduca, M. A., Branisteanu, D., Serban, D. N., Branisteanu, D. C., Stoleriu, G., Manolache, N. and Serban, I. L. (2019) Synthesis and physiological implications of melanic pigments. Oncol. Lett. 17: 4183-4187.
13 Bonaventure, J., Domingues, M. J. and Larue L. (2013) Cellular and molecular mechanisms controlling the migration of melanocytes and melanoma cells. Pigment Cell Melanoma Res. 26: 316-325.   DOI
14 D'Mello, S. A., Finlay, G. J., Baguley, B. C. and Askarian-Amiri, M. E. (2016) Signaling pathways in melanogenesis. Int. J. Mol. Sci. 17: 1144.   DOI
15 Gastebois, A., Clavaud, C., Aimanianda, V. and Latge, J. P. (2009) Aspergillus fumigatus: cell wall polysaccharides, their biosynthesis and organization. Future Microbiol. 4: 583-595.   DOI
16 Park, S. H., Kim, D. S., Kim, W. G., Ryoo, I. J., Lee, D. H., Huh, C. H., Youn, S. W., Yoo, I. D. and Park, L. C. (2004) Terrein: a new melanogenesis inhibitor and its mechanism. Cell Mol. Life Sci. 61: 2878-2885.   DOI
17 Tai, A., Ohno, A. and Ito, H. (2016) Isolation and characterization of the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation-scavenging reaction products of arbutin. J. Agric. Food Chem. 64: 7285-7290.   DOI
18 Burnett, C. L., Bergfeld, W. F., Belsito, D. V., Hill, R. A., Klaassen, C. D., Liebler, D. C., Marks, J. G. Jr., Shank, R. C., Slaga, T. J., Snyder, P. W. and Andersen, F. A. (2010) Final report of the safety assessment of kojic acid as used in cosmetics. Int. J. Toxicol. 29: 244-273.
19 Battaini, G., Monzani, E., Casella, L., Santagostini, L. and Pagliarin, R. (2000) Inhibition of the catecholase activity of biomimetic dinuclear copper complexes by kojic acid. J. Biol. Inorg. Chem. 5: 262-268.   DOI
20 Kim, K. Y. and Lee, N. K. (2014) Herbal extracts research trend that have effects on melanin production and control. Kor. J. Aesthet. Cosmetol. 12: 453-461.
21 Park, H. S. and Yu, J. H. (2016) Developmental regulators in Aspergillus fumigatus. J. Microbiology. 54: 223-231.   DOI
22 Sugimoto, K., Nishimura, T., Nomura, K., Sugimoto, K. and Kurik, T. (2014) Inhibitory effects of α-arbutin on melanin synthesis in cultured human melanoma cells and a three-dimensional human skin model. Biol. Pharm. Bull. 27: 510-514.   DOI
23 Hearing, V. J. and Ekel, T. M. (1973) Mammalian tyrosinase. A comparison of tyrosine hydroxylation and melanin formation. Biochem. J. 157: 549-557.   DOI
24 Park, J. M. and Kim, K. J. (2010) The anti-wrinkle effects and whitening effects of Galla Rhois. J. Kor. Ori. Med. Opht. Otol. 23: 135-148.
25 Agar, N. and Young, A. R. (2005) Melanogenesis: a photoprotective response to DNA damage? Mutation Research. 571: 121-132.   DOI
26 Fuller, B. B. and Meyskens, F. L. Jr. (1981) Endocrine responsiveness in human melanocytes and melanoma cells in culture. J. Natl. Cancer Inst. 66: 799-802.
27 Takechi, Y., Hara, I., Naftzger, C., Xu, Y. and Houghton, A. N. (1996) A melanosomal membrane protein is a cell surface target for melanoma therapy. Clin. Cancer Res. 2: 1837-1842.