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http://dx.doi.org/10.22889/KJP.2020.51.4.349

Effect of Coptidis Rhizoma and Evodiae Fructus Mixture on Esophageal Mucosa in Chronic Reflux Esophagitis  

Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Lee, Ji Hye (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
Roh, Seong-soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Publication Information
Korean Journal of Pharmacognosy / v.51, no.4, 2020 , pp. 349-359 More about this Journal
Abstract
Gastroesophageal reflux disease (GERD) is a disease that stomach contents continually refluxing, and is currently on the rise worldwide. The purpose of this study is to find natural materials that can reduce side effects and effectively treat chronic acid reflux esophagitis (CARE), one of GERD. First, the antioxidant activity was confirmed by varying the mixing ratio of Coptidis Rhizoma and Evodiae Fructus, which are effective against chronic reflux esophagitis. After, animal experiments were conducted using a 1:1 (CE) and 1:2 (CEE) combination ratio of Coptidis Rhizoma and Evodiae Fructus, which had the best antioxidant efficacy. Gross lesion of esophageal mucosa after CE or CEE treatment showed a superior enhancement compared with that of CARE control rats. Additionally, its inhibited MAPK phosphorylation and led NF-κB inactivation through the suppression of IκBα phosporylation by regulating Nrf2/Keap-1, and NF-κB inactivation induced reduced protein expressions including inflammatory mediators and cytokines. Moreover, its improved esophageal barrier function through upregulating protein expressions of tight junction protein, whereas downregulating protein expressions of MMPs. Taken together, a mixture of Coptidis Rhizoma and Evodiae Fructus can attenuate the esophageal mucosal ulcer by inhibiting MAPK and NF-κB pathway, and upregulating proteins associated with tight junction.
Keywords
Chronic Reflux Esophagitis; Coptidis Rhizoma; Evodiae Fructus; Inflammation; Tight junction;
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