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http://dx.doi.org/10.22889/KJP.2020.51.4.317

Swertia pseudochinensis Methanol Extract Inhibits IgE-mediated Allergic Response In vitro and In vivo  

Jeon, Sun Ha (College of Pharmacy, Duksung Women's University)
Kim, Young Mi (College of Pharmacy, Duksung Women's University)
Publication Information
Korean Journal of Pharmacognosy / v.51, no.4, 2020 , pp. 317-324 More about this Journal
Abstract
Mast cells play a key role in IgE-mediated allergic response. We investigated whether Swertia pseudochinensis Hara extract (SPE) inhibits IgE-mediated allergic response in mast cells and an allergic animal model. Additionally, we explored SPE's mechanism of action in mast cells. Our results showed that SPE inhibited both antigen-stimulated degranulation and the production of TNF-α and IL-4 in bone marrow-derived mast cells (BMMCs) and rat basophilic leukemia (RBL)-2H3 cells. SPE also suppressed allergic response in IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. As for the mechanism of action of SPE in mast cells, it inhibited the activation of Syk kinase, a critical signaling protein in the FcεRI-mediated signaling pathway, and also the activation of LAT, a downstream adaptor protein of Syk. We further observed the reduced activation of mitogen-activated protein (MAP) kinases (P38, ERK1/2, and JNK) and Akt in mast cells. Our results described for the first time that SPE has an anti-allergic effect by suppressing mast cells through the inhibition of Syk kinase. Therefore, SPE may be useful for the treatment of type I allergic diseases.
Keywords
Swertia pseudochinensis; Mast cell; Passive cutaneous anaphylaxis; Allergy; Syk;
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