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Anti-inflammatory Effects of the Methanol Extracts of Phlox subulata on LPS-induced RAW264.7 Macrophages and BV2 Microglia  

Kim, Kwan-Woo (College of Pharmacy, Wonkwang University)
Li, Jing (College of Pharmacy, Wonkwang University)
Lee, Hwan (College of Pharmacy, Chosun University)
Lee, Dong-Sung (College of Pharmacy, Chosun University)
Oh, Hyuncheol (College of Pharmacy, Wonkwang University)
Kim, Youn-Chul (College of Pharmacy, Wonkwang University)
Publication Information
Korean Journal of Pharmacognosy / v.50, no.4, 2019 , pp. 291-298 More about this Journal
Abstract
Phlox subulata is a perennial herbaceous flower and is a member of the Polemoniaceae family. This plant is known to resist to various stresses including salt, drought, heat, and cold stresses. In this investigation, we evaluated the ant-inflammatory effect of the methanolic extract of P.subulata(PSM) on lipopolysaccharide(LPS)-induced RAW264.7 macrophages and BV2 microglia. PSM reduced the production of nitric oxide(NO) in LPS-stimulated both RAW264.7 and BV2 cells, but did not affect to the production of prostaglandin E2(PGE2). It inhibited the expression of inducible nitric oxide synthase(iNOS) and cyclooxygenase(COX)-2 in both cells. In addition, PSM suppressed the production of pro-inflammatory cytokines including interleukin(IL)-6 and tumor necrosis factor(TNF)-α. These inhibitory effects were contributed by inactivation of nuclear factor kappa B(NF-κB) and mitogen-activated protein kinases(MAPKs) pathways by PSM. Thus, these results suggested that P.subulata can be a candidate material to treat inflammatory diseases.
Keywords
Phlox subulata; Lipopolysaccharide; Inflammation; RAW264.7 macrophage; BV2 microglia;
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