Browse > Article

Anti-aging Effect of Cycloheterophyllin in UVA-irradiated Dermal Fibroblasts  

Shim, Joong Hyun (Faculty of Cosmetics & Beauty biotechnology, Semyung University)
Publication Information
Korean Journal of Pharmacognosy / v.50, no.4, 2019 , pp. 285-290 More about this Journal
Abstract
This study was carried out to identify the skin anti-aging effect of cycloheterophyllin on dermal fibroblasts. To elucidate anti-aging effects of cycloheterophyllin on dermal fibroblasts, I measured cell viability, mRNA expressions, and Collagen, type I/matrix metallopeptidase 1(MMP1)-ELISA assay. In this study, I investigated the effects of cycloheterophyllin on Collagen, type I, alpha 1(COL1A1)/Collagen, type III, alpha 1(COL3A1)/MMP1/Superoxide dismutases/Catalase(CAT) mRNA expressions and Collagen, type I/MMP1 protein production. Quantitative Real-time RT-PCR showed that cycloheterophyllin increased mRNA level of COL1A1/COL3A1/CAT genes and collagen, type I protein by ELISA assay compared to UVA-treated dermal fibroblasts. Furthermore MMP1 mRNA and protein expressions were decreased by cycloheterophyllin treatment. These observations revealed that cycloheterophyllin increased anti-aging effects in dermal fibroblasts. Therefore, I identified the anti-aging effects of cycloheterophyllin, and these results showed that the cycloheterophyllin can be a considerable potent ingredient for skin anti-aging. Based on this, I anticipated further researches about cycloheterophyllin for mechanism to develop not only cosmetics but for healthcare food or medicine.
Keywords
Cycloheterophyllin; Anti-aging; Collagen; type I; alpha 1; Matrix metallopeptidase 1; Collagen; type III; alpha 1; Catalse;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lee, H. Y., Kim, K. J. and Kim, Y. S. (2007) Skin science, Gunja Publishing, pp. 20-24, Seoul.
2 Yoon, Y. M., Bae, S. H., An, S. K., Choe, Y. B., Ahn, K. J. and An, I. S. (2013) Effects of ultraviolet radiation on the skin and skin cell signaling pathways. Kor. J. Aesthet. Cosmetol. 11: 417-426.
3 Kim, J., Lee, C. W., Kim, E. K., Lee, S. J., Park, N. H., Kim, H. S., Kim, H. K., Char, K., Jang, Y. P. and Kim, J. W. (2011) Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts. J. Enthnopharmacol. 137: 427-433.   DOI
4 Gilchrest, B. A. (1996) A review of skin ageing and its medical therapy. Br. J. Dermatol. 135: 867-875.   DOI
5 Chung, J. H., Seo, J. Y., Lee, M. K., Eun, H. C., Lee, J. H,, Kang, S., Fisher, G. J. and Voorhees, J. J. (2002) Ultraviolet modulation of human macrophage metalloelastase in human skin in vivo. J. Invest. Dermatol. 119: 507-512.   DOI
6 Brenneisen, P., Sies, H. and Scharffetter-Kochanek, K. (2002) Ultraviolet-B irradiation and matrix metalloproteinases: from induction via signaling to initial events. Ann. N. Y. Acad. Sci. 973: 31-43.   DOI
7 Assefa, Z., Van, Laethem A., Garmyn, M. and Agostinis, P. (2005) Ultraviolet radiation-induced apoptosis in keratinocytes: on the role of cytosolic factors. Biochim. Biophys. Acta. 1755: 90-106.
8 Bickers, D. R. and Athar, M. (2006) Oxidative stress in the pathogenesis of skin disease. J. Invest. Dermatol. 126: 256-275.
9 Moysan, A., Marquis, I., Gaboriau, F., Santus, R., Dubertret, L. and Morliere, P. (1993) Ultraviolet A-induced lipid peroxidation and antioxidant defense systems in cultured human skin fibroblasts. J. Invest. Dermatol. 100: 692-698.   DOI
10 Poswig, A., Wenk, J., Brenneisen, P., Wlaschek, M., Hommel, C., Quel, G., Faisst, K., Dissemond, J., Briviba, K., Krieg, T. and Scharffetter-Kochanek, K. (1999) Adaptive antioxidant response of manganese-superoxide dismutase following repetitive UVA irradiation. J. Invest. Dermatol. 112: 13-18.   DOI
11 Lin, C. N., Lu, C. M., Lin, H. C., Fang, S. C., Shieh, B. J. and Hsu, M. F. (1996) Novel antiplatelet constituents from Formosan moraceous plants. Journal of Natural Products 59: 834-838.   DOI
12 Jantan, I., Mohd, Yasin. Y. H., Jamil, S., Sirat, H. and Basar, N. (2010) Effect of prenylated flavonoids and chalcones isolated from Artocarpus species on platelet aggregation in human whole blood. Journal of Natural Medicines 64: 365-369.   DOI
13 Wang, J. P., Raung, S. L., Tsao, L. T., Hsu, M. F. and Lin, C. N. (1997) Blockade of protein kinase C is involved in the inhibition by cycloheterophyllin of neutrophil superoxide anion generation. Naunyn-Schmiedeberg's Archives of Pharmacology 355: 551-558.   DOI
14 Ko, F. N., Cheng, Z. J., Lin, C. N. and Teng, C. M. (1998) Scavenger and antioxidant properties of prenylflavones isolated from Artocarpus heterophyllus. Free Radical Biology & Medicine 25: 160-168.   DOI
15 Huang, C. H., Li, H. J., Wu, N. L., Hsiao, C. Y., Lin, C. N., Chang, H. H. and Hung, C. F. (2016) Photoprotective effects of cycloheterophyllin against UVA-induced damage and oxidative stress in human dermal fibroblasts. PloS One 11: e0161767.   DOI
16 Kirkwood, T. B. (2005) Understanding the odd science of aging. Cell. 120: 437-447.   DOI
17 Shim, J. H. (2017) Anti-aging effects of P7C3 in UVA-irradiated human dermal fibroblasts. Asian Journal of Beauty and Cometology 15: 45-53.   DOI
18 Fukai, T. and Ushio-Fukai, M. (2011) Superoxide dismutases: role in redox signaling, vascular function, and diseases. Antioxid Redox Signal 15: 1583-1606.   DOI
19 Talwar, H. S., Griffiths, C. E., Fisher, G. J., Hamilton, T. A. and Voorhees, J. J. (1995) Reduced type I and type III procollagens in photodamaged adult human skin. J. Invest. Dermatol. 105: 285-290.   DOI
20 Jones, D. L. and Rando, T. A. (2011) Emerging models and paradigms for stem cell ageing. Nat. Cell Biol. 13: 506-512.   DOI