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Isolation and Quantitative Analysis of Naringenin, Sakuranetin and Prunetin from the Barks of Prunus yedoensis  

Kim, Eun-Nam (College of Pharmacy, Keimyung University)
Kim, Sung-Cheol (College of Pharmacy, Keimyung University)
Jeong, Gil-Saeng (College of Pharmacy, Keimyung University)
Publication Information
Korean Journal of Pharmacognosy / v.50, no.3, 2019 , pp. 226-231 More about this Journal
Abstract
Prunus yedoensis Matsum. (Rosaceae) has been used for cough, urticaria, pruritus, dermatitis, asthma and relaxation in traditional Korean medicine. In this study, naringenin, sakuranetin and prunetin were isolated from the barks of P. yedoensis and quantification were performed by using high performance liquid chromatography (HPLC) method with diode array detector. The structures of naringenin and sakuranetin, prunetin were identified NMR spectroscopic data analysis. The content of each compound was analyzed by HPLC and the analytical method was validated through linearity, precision, accuracy and specificity test. The result showed that calibration curves of three compounds naringenin, sakuranetin and prunetin indicated great linearity with a correlation coefficient ($R^2$) of 1.00, 1.00 and 0.99. Intra and inter day measurement accuracy of the three compounds ranged from 92.70 ~ 112.70%, and showed precision was less than 3%. Therefore, the content analysis showed that naringenin ($0.132{\pm}0.016%$), sakuranetin ($0.108{\pm}0.023%$), and prunetin ($0.059{\pm}0.014%$).
Keywords
HPLC; Prunus yedoensis Matsum.; Sakuranetin; Naringenin; Prunetin;
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1 Grecco, S. D. S., Reimao, J. Q., Tempone, A. G., Sartorelli, P., Cunda R. L. O. R., Romoff, P., Ferreira, M. J. P., Favero, O. A. and Lago, J. H. G. (2011) In vitro antileishmanial and antitrypanosomal activities of flavanones from Baccharis retusa DC. (Asteraceae). J. Exppara. 130: 141-145.
2 Innan, H., Terauchi, R., Miyashita, N. T. and. Tsunewaki K. (1995) DNA fingerprinting study on the intraspecific variation and the origin of Prunus yedoensis (Someiyoshino). Jpn. J. Genet. 70: 185-196.   DOI
3 Roh, M. S., Cheong, E. J., Choi, I. Y. and Joung. Y. H. (2007) Characterization of wild Prunus yedoensis analyzed by intersimple sequence repeat and chloroplast DNA. Sci. Hortic. 114: 121-128.   DOI
4 Lee, K. J., Ham, I. H., Yang, G. S., Lee, M. h., Bu, Y. M., Kim, H. C. and Choi, H.Y. (2013) Vasorelaxant effect of Prunus yedoensis bark. BMC. Complement Altern. Med. 13: 31.   DOI
5 Yang, S. A., Cho, J. H., Pyo, B. S., Kim, S. M. and Lee, K. I. (2012) Comparison of the physiological activities of extracts from different parts of Prunus sargentii. Korean J. Medicinal Crop. Sci. 20: 159-164.   DOI
6 Cho, M. S., Kim, C. S., Kim, S. H. and Kim, S. C. (2016) Comparative phylogenetic relationship between wild and cultivated Prunus yedoensis Matsum. (Rosaceae) with regard to Taquet's collection. Korean J. Pl. Taxon. 46: 247-255.   DOI
7 Jung, H. A., Kim, A. R., Chung, H. Y. and Choi, J. S. (2002) In vitro antioxidant activity of some selected Prunus species in Korea. Arch. Pharm. Res. 25: 865-872.   DOI
8 Jo, K., Lee, S. E., Lee, S. W. and Hwang, J. K. (2012) Prunus yedoensis Matsum. stimulates glucose uptake in L6 rat skeletal muscle cells by activating AMP-activated protein kinase and phosphatidylinositol 3-kinase/Akt pathways. Nat. Prod. Res. 26: 1610-1615.   DOI
9 Kang, G. J., Lee, H. J., Yoon, W. J., Yang, E. J., Park, S. S., Kang, H. K., Park, M. H. and Yoo, E. S. (2008) Prunus yedoensis inhibits the inflammatory chemokines, MDC and TARC, by regulating the STAT1-signaling pathway in IFN-g-stimulated HaCaT human keratinocytes. Biomol. Ther. 16: 394-402.   DOI
10 Lee, B. B., Cha, M. R., Kim, S. Y., Park, E. J., Park, H. R. and Lee, S. C. (2007) Antioxidative and anticancer activity of extracts of cherry (Prunus serrulata var. spontanea) blossoms. Plant Foods Hum. Nutr. 62: 79-84.   DOI
11 Zhang, Y. Q., Yin, C., Zhang, S., Chang, M. S., Tang, J. W. and Guan, L (2013) Anti-oxidative activity and safety of cherry blossom extracts in vitro. J. Diagn. Ther. Derma. Venereol. 20: 392-395.
12 Zhang, Y. Q., Guan, L., Zhong, Z. Y., Chang, M., Zhang. D. K., Li. H. and Lai W. (2014) The anti-inflammatory effect of cherry blossom extract (Prunus yedoensis) used in soothing skincare product. Int. J. Cosmet. Sci. 36: 527-530.   DOI
13 Jeong, M. Y., Im, S. B., Roh, M. K., Park, S. H., Kwon, H. A., Lee, J. Y., Choi, H. Y., Ham, I. H., Kim, Y. B., Lee, J. M., Kim, D. O., Park, K.W. and Kang H. (2014) Prunus yedoensis bark inhibits lipopolysaccharide-induced inflammatory cytokine synthesis by I?B? degradation and MAPK activation in macrophages. J. Med. Food. 17: 407-413.   DOI
14 Rice, E. C., Miller, N. J., Bolwell, P. G., Bramley, P. M. and Pridham, J. B. (1995) The relative antioxidant activities of plant-derived polyphenolic flavonoids. Free Radic. Res. 22: 375-383.   DOI
15 Zeng, W., Jun, L., Zhang, F., Zhang, C. and Liang, W. (2018) Naringenin as a potential immunomodulator in therapeutics. Pharmacol. Res. 135: 122-126.   DOI
16 Yuko, Y., Tetsuya, O., Toshinari, I., Tadayoshi, O., Yukinobu, I. and Mikio, N. (2018) Sakuranetin downregulates inducible nitric oxide synthase expression by affecting interleukin-1 receptor and CCAAT/enhancer-binding protein ${\beta}$. J. Nat. Med. 73: 353-368.
17 Ahn, T. Gue., Yang, G. S., Lee, H. M., Kim, M. D., Choi, H. Y., Park, K. S., Lee, S. D., Kook, Y. B. and An, H. J. (2013) Molecular mechanisms underlying the anti-obesity potential of prunetin, an O-methylated isoflavone. Biochem. Pharmacol. 85: 1525-1533.   DOI
18 Zhang, L., Xu, X., Jiang, T., Wu, K., Ding, C., Liu, Z., Zhang, X., Yu, T. and Song, C. (2018) Citrus aurantium naringenin prevents osteosarcoma progression and recurrence in the patients who underwent osteosarcoma surgery by improving antioxidant capability. Oxid. Med. Cell Longev. 2018: 16.
19 Burke, A. C., Sutherland, B. G., Telford, D. E., Morrow, M. R., Sawyez, C. G., Edwards, J. Y. and Huff M. W. (2009) Naringenin enhances the regression of atherosclerosis induced by a chow diet in Ldlr-/- mice. Atherosclerosis. 286: 60-70.   DOI
20 Jesus, F., Concalves, A. C., Alves, G. and Silva, L. R. (2018) Exploring the phenolic profile, antioxidant, antidiabetic and antihemolytic potential of Prunus avium vegetal parts. J. Food Res. 116: 600-610.
21 Yun, J. M., Im, S. B., Roh, M. K., Park, S. H., Kwon, H. A.,1 Lee, J. Y., Choi, H. Y., Ham, I. H., Kim. Y. B., Lee, J. M., Kim, D. O., Park, K. W. and Kang, H. (2014) Prunus yedoensis bark Inhibits lipopolysaccharide-induced inflammatory cytokine synthesis by I${\kappa}B$${\alpha}$ degradation and MAPK activation in macrophages. J. Med. Food. 17: 407-413.   DOI
22 Sahu, A. K. and Jain, V. (2015) Quantification of naringenin encapsulated in solid lipid nanoparticles by validated UVspectroscopy method. Planta Med. 81: 13.