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Aged Doraji (Platycodon grandiflorum) Ameliorates Cyclophosphamide-Induced Immunosuppression in Mice  

Choi, Ji-Hye (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Eun Byeol (National Institute of Agricultural Sciences, Rural Development Administration)
Park, Yang-Gyu (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Hye Kyung (National Institute of Agricultural Sciences, Rural Development Administration)
Jang, Hwan Hee (National Institute of Agricultural Sciences, Rural Development Administration)
Choe, Jeongsook (National Institute of Agricultural Sciences, Rural Development Administration)
Hwang, Kyung-A (National Institute of Agricultural Sciences, Rural Development Administration)
Park, Shin Young (National Institute of Agricultural Sciences, Rural Development Administration)
Hwang, In Guk (National Institute of Agricultural Sciences, Rural Development Administration)
Hong, Ha Cheol (National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Hae-Jeung (Department of Food and Nutrition, Gachon University)
Jeong, Hyun Cheol (Food R&D Center, SK bioland Co. Ltd.)
Kim, Hyun-Ju (World Institute of Kimchi)
Lee, Sung Hyen (National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Korean Journal of Pharmacognosy / v.50, no.3, 2019 , pp. 219-225 More about this Journal
Abstract
This study was done to examine immunomodulative effects of aged doraji (AD) in the immune-suppressed mice induced by cyclophosphamide. The immune-stimulating effects of ethanol AD extract in in vivo at 75 and 150 mg/kg body weight (BW) for AD and 2AD groups were evaluated and compared to the normal doraji group (2ND, 150 mg/kg BW) treated with a doraji without aging process. After the 10 days of oral supplement, body and immune related organ weights, serum immunoglobulin G (IgG) and cytokines levels, splenocytes proliferation rate, and splenic NK cell activity were measured as immune-related biomarkers. Body weight and serum IgG level increased in the 2AD group. But, the serum Th2 cytokine (IL-6, $TNF-{\alpha}$) levels were lower in the AD and 2AD groups, respectively. Splenic T cell and B cell proliferation and NK cell activity increased in the doraji groups and the significant increases were found only in the 2AD group. Thus, the aged doraji extract may affect body weight, serum IgG level, splenocytes proliferation, and splenic NK cell activity, and normalize the Th2 cytokine levels in the immune-suppressed mice. The results suggest that the aged doraji improves effectively immune system rather than the normal one.
Keywords
Platycodon grandiflorum; Immune; Splenocyte proliferation; Serum cytokine; NK cell activity;
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Times Cited By KSCI : 13  (Citation Analysis)
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