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Broussochalcone B from Broussonetia papyrifera Induce Apoptosis via Activation of a Caspase Cascade and Reactive Oxygen Species Production in Human HepG2 cells  

Park, Jin Ryang (Department of Life Science, Seonam University)
Ryu, Hyung Won (Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology)
Cho, Byoung Ok (Department of Health Care & Management, Jeonju University)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.4, 2017 , pp. 273-279 More about this Journal
Abstract
The aim of this study was to investigate the mechanisms underlying apoptosis induced by a broussochalcone B (BCB) from Broussonetia papyrifera in HepG2 cells. The results showed that BCB treatment for 24 hr significantly inhibited cell viability in a dose-dependent manner, and induced apoptosis in HepG2 cells. More so, BCB treatment triggered the cleavage of caspase-8, -9, -3, poly (ADP-ribose) polymerase (PARP), increase of Bax level, and decrease of Bcl-2 expression. A general caspase inhibitor (z-VAD-fmk) blocked BCB-induced cell death. Furthermore, BCB treatment caused reactive oxygen species (ROS) production in a dose-dependent manner. In addition, an antioxidant N-acetylcysteine (NAC) blocked BCB-induced ROS production and cell death. Therefore, these results indicate that BCB-induced apoptosis is mediated by a caspase dependent pathway and ROS production in HepG2 cells.
Keywords
Broussochalcone B; Broussonetia papyrifera; Apoptosis; Caspase; Reactive oxygen species;
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1 Kang, H. I., Kim, J. Y., Cho, H. D., Park, K. W., Kang, J. S. and Seo, K. I. (2010) Resveratrol induces apoptosis in primary human prostate cancer cells. J. Korean Soc. Food Sci. Nutr. 39: 1119-1125.   DOI
2 Park, S. Y., Kim, J. Y., Park, K. W., Kang, K. S., Park K. H. and Seo, K. I. (2009) Effects of thiosulfinates isolated from Allium tuberosum L. on the growth of human cancer cells. J. Korean Soc. Food Sci. Nutr. 38: 1003-1007.   DOI
3 Kim, N. (2008) Butein-induced apoptosis in human T lymphoma Jurkat cells. Kor. J. Pharmacogn. 39: 150-154.
4 Sohn, H. Y., Son, K. H., Kwon, C. S., Kwon, G. S. and Kang, S. S. (2004) Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine. 11: 666-672.   DOI
5 Sohn, H. Y., Kwon, C. S. and Son, K. H. (2010) Fungicidal effect of prenylated flavonol, papyriflavonol A, isolated from Broussonetia papyrifera (L.) vent. against Candida albicans. J. Microbiol. Biotechnol. 20: 1397-1402.   DOI
6 Guo, F., Feng, L., Huang, C., Ding, H., Zhang, X., Wang, Z., Li, Y. (2013) Prenylflavone derivatives from Broussonetia papyrifera, inhibit the growth of breast cancer cells in vitro and in vivo. Phytochem. lett. 6: 331-336.   DOI
7 Nobili, S., Lippi, D., Witort, E., Donnini, M., Bausi, L., Mini, E. and Capaccioli, S. (2009) Natural compounds for cancer treatment and prevention. Pharmacol. Res. 59: 365-378.   DOI
8 Choi, J. H., Kim, H. I. and Lee, I. S. (2009) Effect of Rosmarinus officinalis L. on growth inhibition and apoptosis induction in cancer cells. J. Korean Soc. Food Sci. Nutr. 38: 1008-1015.   DOI
9 Yang, C., Li, F., Du, B., Chen, B., Wang, F. and Wang, M. (2014) Isolation and characterization of new phenolic compounds with estrogen biosynthesis-inhibiting and antioxidation activities from Broussonetia papyrifera leaves. PLoS One. 9: e94198.   DOI
10 Park, S., Fudhaili, A., Oh, S. S., Lee, K. W., Madhi, H., Kim, D. H., Yoo, J., Ryu, H. W., Park, K. H. and Kim, K. D. (2016) Cytotoxic effects of kazinol A derived from Broussonetia papyrifera on human bladder cancer cells, T24 and T24R2. Phytomedicine. 23: 1462-1468.   DOI
11 Pang, S. Q., Wang, G. Q., Lin, J. S., Diao, Y. and Xu, R. A. (2014) Cytotoxic activity of the alkaloids from Broussonetia papyrifera fruits. Pharm Biol. 52: 1315-1319.   DOI
12 Chae, Y. H., Shin, D. Y., Park, C., Lee, Y. T., Moon, S. G. and Choi, Y. H. (2011) Induction of apoptosis in human colon carcinoma HCT116 cells using a water extract of Lepidium virginicum L. J. Korean Soc. Food Sci. Nutr. 40: 649-659.   DOI
13 Tan, M. L., Ooi, J. P., Ismail, N., Moad, A. I. H. and Muhammad, T. S. T. (2009) Programmed cell death pathways and current antitumor targets. Pharm. Res. 26: 1547-1560.   DOI
14 Fulda, S. (2009) Caspase-8 in cancer biology and therapy. Cancer Lett. 281: 128-133.   DOI
15 Mellier, G., Huang, S., Shenoy, K. and Pervaiz, S. (2010) TRAILing death in cancer. Mol. Aspects Med. 31: 93-112.   DOI
16 Kim, J. E., Chung, W. Y., Chun, K. S., Lee, C. K., Park, H. J., Kim, W. B. and Park, K. K. (2010) Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells. Biosci. Biotechnol. Biochem. 74: 788-792.   DOI
17 Li-Weber, M. (2013) Targeting apoptosis pathways in cancer by Chinese medicine. Cancer Lett. 332: 304-312.   DOI
18 Cho, B. O., Ryu, H. W., So, Y. K., Jin, C. H., Byun, M. W., Kim, W. G. and Jeong, I. Y. (2012) Ishige sinicola extracts induce apoptosis via activation of a caspase cascade in human HeLa cells. J. Korean Soc. Food Sci. Nutr. 41: 901-906.   DOI
19 Lin, L. W., Chen, H. Y., Wu, C. R., Liao, P. M., Lin, Y. T., Hsieh, M. T. and Ching, H. (2008) Comparison with various parts of Broussonetia papyrifera as to the antinociceptive and anti-inflammatory activities in rodents. Biosci. Biotechnol. Biochem. 72: 2377-2384.   DOI
20 Cao, X. H., Wang, A. H., Wang, C. L., Mao, D. Z., Lu, M. F., Cui, Y. Q. and Jiao, R. Z. (2010) Surfactin induces apoptosis in human breast cancer MCF-7 cells through a ROS/JNKmediated mitochondrial/caspase pathway. Chem. Biol. Interact. 183: 357-362.   DOI
21 Zhu, Y., Jiang, Y., Shi, L., Du, L., Xu, X., Wang, E., Sun, Y., Guo, X., Zou, B., Wang, H., Wang, C., Sun, L. and Zhen, Y. (2017) 7-O-Geranylquercetin induces apoptosis in gastric cancer cells via ROS-MAPK mediated mitochondrial signaling pathway activation. Biomed. Pharmacother. 87: 527-538.   DOI
22 Ding, Y., Ren, K., Dong, H., Song, F., Chen, J., Guo, Y., Liu, Y., Tao, W. and Zhang, Y. (2017) Flavonoids from persimmon (Diospyros kaki L.) leaves inhibit proliferation and induce apoptosis in PC-3 cells by activation of oxidative stress and mitochondrial apoptosis. Chem. Biol. Interact. 275: 210-217.   DOI
23 Wang, Q., Wang, H., Jia, Y., Pan, H. and Ding, H. (2017) Luteolin induces apoptosis by ROS/ER stress and mitochondrial dysfunction in gliomablastoma. Cancer Chemother. Pharmacol. 79: 1031-1041.   DOI
24 Su, CH., Kuo, C. L., Lu, K. W., Yu, F. S., Ma, Y. S., Yang, J. L., Chu, Y. L., Chueh, F. S., Liu, K. C. and Chung, J. G. (2017) Fisetin-induced apoptosis of human oral cancer SCC-4 cells through reactive oxygen species production, endoplasmic reticulum stress, caspase-, and mitochondria-dependent signaling pathways. Environ. Toxicol. 32: 1725-1741.   DOI
25 Lin, C. N., Lu, C. M., Lin, H. C., Fang, S. C., Shieh, B. J., Hsu, M. F., Wang, J. P., Ko, F. N. and Teng, C. M. (1996) Novel antiplatelet constituents from formosan moraceous plants. J. Nat. Prod. 59: 834-838.   DOI
26 Sun, J., Liu, S. F., Zhang, C. S., Yu, L. N., Bi, J., Zhu, F. and Yang, Q. L. (2012) Chemical composition and antioxidant activities of Broussonetia papyrifera fruits. PLoS One. 7: e32021.   DOI
27 Ryu, H. W., Lee, B. W., Curtis-Long, M. J., Jung, S., Ryu, Y. B., Lee, W. S. and Park, K. H. (2010) Polyphenols from Broussonetia papyrifera displaying potent alpha-glucosidase inhibition. J. Agric. Food Chem. 58: 202-228.   DOI
28 Ko, H. H., Yu, S. M., Ko, F. N., Teng, C. M. and Lin, C. N. (1997) Bioactive constituents of Morus australis and Broussonetia papyrifera. J. Nat. Prod. 60: 1008-1011.   DOI
29 Chen, R. M., Hu, L. H., An, T. Y., Li, J. and Shen, Q. (2002) Natural PTP1B inhibitors from Broussonetia papyrifera. Bioorg. Med. Chem. Lett. 12: 3387-3390.   DOI
30 Lee, D., Bhat, K. P., Fong, H. H., Farnsworth, N. R., Pezzuto, J. M. and Kinghorn, A. D. (2001) Aromatase inhibitors from Broussonetia papyrifera. J. Nat. Prod. 64: 1286-1293.   DOI