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Steroidal saponins from Dioscorea nipponica Rhizomes and Their Biological Activity  

Park, Kyoung Jin (Natural Product Laboratory, School of Pharmacy, Sungkyunkwan University)
Suh, Won Se (Natural Product Laboratory, School of Pharmacy, Sungkyunkwan University)
Cha, Joon Min (Natural Product Laboratory, School of Pharmacy, Sungkyunkwan University)
Park, Jong Eel (Natural Product Laboratory, School of Pharmacy, Sungkyunkwan University)
Woo, Kyeong Wan (Natural Product Laboratory, School of Pharmacy, Sungkyunkwan University)
Kim, Sun Yeo (College of Pharmacy, Gachon University)
Lee, Kang Ro (Natural Product Laboratory, School of Pharmacy, Sungkyunkwan University)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.4, 2017 , pp. 261-267 More about this Journal
Abstract
As part of the search for bioactive constituents of Korean medicinal plants, twelve steroids (1-12) were isolated from the rhizomes of Dioscorea nipponica. The isolated compounds were identified as diosgenin ($3{\beta}$, 25R)-spirost-5-en-3-ol (1), 25(R)-dracaenoside E (2), dioscin (3), gracillin (4), prosapogenin B (5), 25(R)-dracaenoside G (6), diosgenin 3-O-${\beta}$-D-glucopyranosyl($1{\rightarrow}3$)-${\beta}$-D-glucopyranoside (7), ophipogonin C′ (8), 7-oxodioscin (9), protodioscin (10), hypoglaucin F (11), and protoneogracillin (12). Their structures were characterized by spectroscopic data and identified by comparing these data with those in the literatures. All the isolates (1-12) were evaluated for their neuroprotective effects through induction of nerve growth factor in C6 glioma cells and effects on nitric oxide (NO) production in murine microglia cell line BV-2. Compounds 7 and 12 were found to induce upregulation of NGF secretion without causing significant cell toxicity and compound 4 exhibited potent anti-neuroinflammatory activity.
Keywords
Dioscorea nipponica; Dioscoreaceae; Steroidal saponin; Neuroprotection; Anti-inflammation;
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