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The Mechanism of Crinum asiaticum var. japonicum on the Activation of Anagen  

Kang, Jung-Il (Department of Medicine, School of Medicine, Jeju National University)
Choi, Ju Hwan (Department of Medicine, School of Medicine, Jeju National University)
Lee, Jong Gun (Department of Medicine, School of Medicine, Jeju National University)
Yoo, Eun-Sook (Department of Medicine, School of Medicine, Jeju National University)
Kim, Young Ho (College of Pharmacy, Chungnam National University)
Kang, Hee-Kyoung (Department of Medicine, School of Medicine, Jeju National University)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.2, 2017 , pp. 148-154 More about this Journal
Abstract
Crinum asiaticum var. japonicum and its active component, norgalanthamine have been reported to have hair growth-promoting effect via the proliferation of dermal papilla cells. In this study, we investigated the other mechanisms of C. asiaticum extract var. japonicum and norgalanthamine on the hair growth. The C. asiaticum var. japonicum extract inhibited $5{\alpha}$-reductase activity by 16%, which converts testosterone to dihydrotestosterone (DHT), a main cause of androgenetic alopecia, whereas the C. asiaticum var. japonicum extract didn't function as an opener of the $K_{ATP}$ channel. On the other hand, we examined whether norgalanthamine can inhibit transforming growth factor-${\beta}$ (TGF-${\beta}$) signal pathway, which is essential in the regression induction of hair growth. Norgalanthamine inhibited the phosphorylation of Smad2/3 on TGF-${\beta}1$-induced canonical pathway in human keratinocyte HaCaT cells. These results suggested that the C. asiaticum var. japonicum extract and norgalanthamine had the potential to influence hair growth through the inhibition of $5{\alpha}$-reductase activity and TGF-${\beta}1$-induced canonical pathway.
Keywords
Crinum asiaticum var. japonicum; Norgalanthamine; $5{\alpha}$-Reductase; HaCaT keratinocytes; TGF-${\beta}1$; Smad2/3;
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1 Price, V. H. (1999) Treatment of hair loss. N. Engl. J. Med. 341: 964-973.   DOI
2 Ellis, J. A., Sinclair, R. and Harrap, S. B. (2002) Androgenetic alopecia: pathogenesis and potential for therapy. Expert. Rev. Mol. Med. 4: 1-11.
3 Elliott, K., Stephenson, T. J. and Messenger, A. G. (1999) Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses. J. Invest. Dermatol. 113: 873-877.   DOI
4 Kaufman, K. D. (2002) Androgens and alopecia. Mol. Cell Endocrinol. 198: 89-95.   DOI
5 Botchkarev, V. A. (2003) Molecular mechanisms of chemotherapy-induced hair loss. J. Investig. Dermatol. Symp. Proc. 8: 72-75.   DOI
6 Aoki, E., Shibasaki, T. and Kawana, S. (2003) Intermittent foot shock stress prolongs the telogen stage in the hair cycle of mice. Exp. Dermatol. 12: 371-377.   DOI
7 Hyun, J. H., Kang, J. I., Kim, S. C., Kim, E., Kang, J. H., Kwon, J. M., Park, D. B., Lee, Y. J., Yoo, E. S. and Kang, H. K. (2008) The effects of Crinum asiaticum on the apoptosis induction and the reversal of multidrug resistance in HL-60/MX2. Toxicol. Res. 24: 29-36.   DOI
8 Kim, Y. H., Park, E. J., Park, M. H., Badarch, U., Woldemichael, G. M. and Beutler, J. A. (2006) Crinamine from Crinum asiaticum var.japonicum inhibits hypoxia inducible factor-1 activity but not activity of hypoxia inducible factor2. Biol Pharm Bull. 29: 2140-2142.   DOI
9 Lo'pez, S., Bastida, J., Viladomat, F. and Codina, C. (2002) Acetylcholinesterase activity of some Amaryllidaceae alkaloids and Narcissus extracts. Life Sci. 71: 2521-2529.   DOI
10 Kim, S. C., Kang, J. I., Kim, M. K., Hyun, J. H., Boo, H. J., Park, D. B., Lee, Y. J., Yoo, E. S., Kim, Y. H., Kim, Y, H. and Kang, H. K. (2010) Promotion effect of norgalanthamine, a component of Crinum asiaticum, on hair growth. Eur. J. Dermatol. 20: 42-48.
11 Picard, F., Schulz, T. and Hartmann, R. W. (2002) 5-phenyl substituted 1-methyl-2-pyridones and 40-substituted biphenyl-4-carboxylic acids. Synthesis and evaluation as inhibitors of steroid-5${\alpha}$-reductase type 1 and 2. Biorg. Med. Chem. 10: 437-448.   DOI
12 Hamaoka, H., Minakuchi, K., Miyoshi, H., Arase, S., Chen, C. H. and Nakaya, Y. (1997) Effect of $K^+$ channel openers on $K^+$ channel in cultured human dermal papilla cells. J. Med. Invest. 44: 73-77.
13 Kaufman, K. D. and Dawber, R. P. (1999) Finasteride, a Type 2 5alpha-reductase inhibitor, in the treatment of men with androgenetic alopecia. Expert. Opin. Investig. Drugs. 8: 403-415.   DOI
14 Messenger, A. G. and Rundegren, J. (2004) Minoxidil: mechanisms of action on hair growth. Br. J. Dermatol. 150: 186-194.   DOI
15 Han, J. H., Kwon, O. S., Chung, J. H., Cho, K. H., Eun, H. C. and Kim, K. H. (2004) Effect of minoxodil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J. Dermatol. Sci. 34: 91-98.   DOI
16 Shorter, K., Farjo, N. P., Picksley, S. M. and Randall, V. A. (2008) Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil. FASEB J. 22: 1725-1736.   DOI
17 Kwack, M. H., Kang, B. M., Kim, M. K., Kim, J. C. and Sung, Y. K. (2011) Minoxidil activates beta-catenin pathway in human dermal papilla cells: A possible explanation for its anagen prolongation effect. J. Dermatol. Sci. 62: 154-159.   DOI
18 Cotsarelis, G. and Millar, S. E. (2001) Towards a molecular understanding of hair loss and its treatment. Trends. Mol. Med. 7: 293-301.   DOI
19 Batchelor, D. (2001) Hair and cancer chemotherapy: consequences and nursing care-a literature study. Eur. J. Cancer. Care(Engl). 10: 147-163.
20 Paus, R., Foitzik, K., Welker, P., Bulfone-Paus, S. and Eichmuller, S. (1997) Transforming growth factor-beta receptor type I and type II expression during murine hair follicle development and cycling. J. Invest. Dermatol. 109: 518-526.   DOI
21 Foitzik, K., Lindner, G., Mueller-Roever, S., Maurer, M., Botchkareva, N., Botchkarev, V., Handjiski, B., Metz, M., Hibino, T., Soma, T., Dotto, G. P. and Paus, R. (2000) Control of murine hair follicle regression (catagen) by TGF-beta1 in vivo. FASEB J. 14: 752-760.   DOI
22 Li, C. Y., Suardet, L. and Little, J. B. (1995) Potential role of WAF1/Cip1/p21 as a mediator of TGF-beta cytoinhibitory effect. J. Biol. Chem. 270: 4971-4974.   DOI
23 Pardali, K., Kurisaki, A., Moren, A., ten Dijke, P., Kardassis, D. and Moustakas, A. (2000) Role of Smad proteins and transcription factor Sp1 in p21(Waf1/Cip1) regulation by transforming growth factor-beta. J. Biol. Chem. 275: 29244-29256.   DOI
24 Park, S. J., Yang, S. W. and Kim, B. C. (2016) Transforming growth factor-${\beta}$1 induces cell cycle arrest by activating atypical cyclin-dependent kinase 5 through up-regulation of Smad3-dependent p35 expression in human MCF10A mammary epithelial cells. Biochem. Biophys. Res. Commun. 472: 502-507.   DOI
25 Cheng, H., Wang, S. and Feng, R. (2016) STIM1 plays an important role in TGF-${\beta}$-induced suppression of breast cancer cell proliferation. Oncotarget.7: 16866-16878.
26 Gabrielsen, B., Monath, T. P., Huggins, J. W., Kefauver, D. F., Petit, G. R., Groszek, G., Hollingshead, M., Kirsi, J. J., Shannon, W. M., Schubert, E. M., Dake, J. and Ugarkar B (1992) Antiviral (RNA) activity of selected Amaryllidaceae isoquinoline constituents and synthesis of related substances J. Nat. Prod. 55: 1569-1581.   DOI
27 Kaufman, K. D., Olsen, E. A., Whiting, D., Savin, R., DeVillez, R., Bergfeld, W., Price, V. H., Van Neste, D., Roberts, J. L., Hordinsky, M., Shapiro, J., Binkowitz, B. and Gormley, G. J. (1998) Finasteride in the treatment of men with androgenetic alopecia. Finasteride Male Pattern Hair Loss Study Group. J. Am. Acad. Dermatol. 39: 578-589.   DOI
28 Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. (1987) Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942.
29 Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.   DOI
30 Min, B. S., Gao, J. J., Nakamura, N., Kim, Y. H. and Hattori, M. (2001) Cytotoxic alkaloids and a flavan from the bulbs of Crinum asiaticum var. japonicum. Chem. Pharm. Bull(Tokyo). 49: 1217-1219.   DOI
31 Sinclair, R. (1998) Male pattern androgenetic alopecia. BMJ. 317:865-869.   DOI
32 Sanders, D. A., Fiddes, I., Thompson, D. M., Philpott, M. P., Westgate, G. E. and Kealey, T. (1996) In the absence of streptomycin, minoxidil potentiates the mitogenic effects of fetal calf serum, insulin-like growth factor 1, and platelet-derived growth factor on NIH 3T3 fibroblasts in a K+ channel-dependent fashion. J. Invest. Dermatol. 107: 229-234.   DOI
33 Olsen, E. A., Hordinsky, M., Whiting, D., Stough, D., Hobbs, S., Ellis, M. L., Wilson, T. and Rittmaster, R. S. (2006) The importance of dual 5alpha-reductase inhibition in the treatment of male pattern hair loss: results of a randomized placebo-controlled study of dutasteride versus finasteride. J. Am. Acad. Dermatol. 55: 1014-1023.   DOI
34 Kumar, N., Rungseevijitprapa, W., Narkkhong, N. A., Suttajit, M. and Chaiyasut, C. (2012) 5${\alpha}$-reductase inhibition and hair growth promotion of some Thai plants traditionally used for hair treatment. J. Ethnopharmacol. 139: 765-771.   DOI
35 Murata, K., Noguchi, K., Kondo, M., Onishi, M., Watanabe, N., Okamura, K. and Matsuda, H. (2013) Promotion of hair growth by Rosmarinus officinalis leaf extract. Phytother. Res. 27: 212-217.   DOI
36 Chiariello, M,, Gomez, E. and Gutkind JS. (2000) Regulation of cyclin-dependent kinase (Cdk) 2 Thr-160 phosphorylation and activity by mitogen-activated protein kinase in late G1 phase. Biochem. J. 349: 869-876.   DOI
37 Kim, S. C., Kang, J. I., Kim, M. K., Boo, H. J., Park, D. B., Lee, Y. K., Kang, J. H., Yoo, E. S., Kim, Y. H. and Kang, H. K. (2011) The hair growth promoting effect of 4-O-methylhonokiol. Eur. J. Dermatol. 21: 1012-1014.
38 Kang, J. I., Kim, S. C., Hyun, J. H., Kang, J. H., Park, D. B., Lee, Y. J., Yoo, E. S. and Kang HK. (2009) Promotion effect of Schisandra nigra on the growth of hair. Eur. J. Dermatol. 19: 119-125.