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Anti-oxidative Effects of Ethyl acetate Fraction of Saururus chinensis in Caenorhabditis elegans  

Lee, Eun Byeol (College of Pharmacy, Woosuk University)
Kim, Jun Hyeong (College of Pharmacy, Woosuk University)
Lee, Jae-Gwang (Co., Ltd. MGWinery)
Han, Sooncheon (College of Pharmacy, Woosuk University)
Park, Hyun Mee (College of Pharmacy, Woosuk University)
Kim, Dae Keun (College of Pharmacy, Woosuk University)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.1, 2017 , pp. 63-69 More about this Journal
Abstract
Saururus chinensis (Lour.) Baill. (Saururaceae) has been used as a traditional medicine for the treatment of dysuria, leukorrhea, eczema, jaundice and ascites in Korea, China and Japan. Ethanol extract of S. chinensis was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions the ethyl acetate soluble fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities of ethyl acetate fraction, we checked the activities of superoxide dismutase (SOD) and catalase, and intracellular ROS level and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of ethyl acetate fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, ethyl acetate fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, ethyl acetate fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.
Keywords
Saururus chinensis; Caenorhabditis elegans; Antioxidant activity;
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