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Antioxidative Activity and Tyrosinase Inhibition Effect of Fractions from the Extract of Inonotus obliquus  

Im, Do-Youn (Division of Liberal Arts and Teacher Training, Kwangju Women's University)
Lee, Kyoung-In (Bio-center, Dongshin University)
Publication Information
Korean Journal of Pharmacognosy / v.48, no.1, 2017 , pp. 46-50 More about this Journal
Abstract
This study was conducted to investigate the antioxidative activities and tyrosinase inhibition effects of fractions from the distilled water extract of Inonotus obliquus. Moreover, GC-MS based analysis with trimethylsilyl (TMS) derivatization was carried out for active compound in the extract of I. obliquus. In DPPH radical scavenging ability, $SC_{50}$ values of the ethyl acetate fraction was 0.393 mg/ml as a result of the most effective than other fractions. Meanwhile, aqueous fraction showed higher effect in tyrosinase inhibitoty activity. In GC-MS based analysis with TMS derivatization, 7 compounds including syringic acid, vanillic acid and protocatechuic acid were observed in ethyl acetate fraction, and oxalic acid is the main compound in aqueous fraction. As a result, it was confirmed that oxalic acid in aqueous fraction from the distilled water extract of I. obliquus was a compound showing tyrosinase inhibition effect.
Keywords
Inonotus obliquus; Antioxidative activity; Tyrosinase; Trimethylsilyl derivatization; GC-MS;
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