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Radical Scavenging Effect and Neuroprotective Activity from Oxidative Stress of Engelhardtia chrysolepis Leaf  

Kim, Eun Jung (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Lee, Ah Young (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Choi, Soo Yeon (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Seo, Hye Rin (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Lee, Young A (Department of Food Science and Nutrition, Catholic University of Daegu)
Cho, Eun Ju (Department of Food Science and Nutrition & Research Institute of Ecology for the Elderly, Pusan National University)
Publication Information
Korean Journal of Pharmacognosy / v.47, no.3, 2016 , pp. 251-257 More about this Journal
Abstract
In this study, the radical scavenging activity and protective effect of ethanol extract from leaf of Engelhardtia chrysolepis HANCE (ECE) against oxidative stress were investigated under in vitro and cellular system. ECE showed strong radical scavenging activities in 1,1-diphenyl-2-picrylhydrazyl, hydroxyl(${\cdot}OH$) and nitric oxide(NO) radical as a concentration-dependent manner. Particularly, strong scavenging activity against the ${\cdot}OH$ and NO radical were observed with the $IC_{50}$ value of $1.30{\mu}g/ml$ and $12.61{\mu}g/ml$, respectively. Furthermore, the cellular oxidative stress was induced by amyloid beta($A{\beta}_{25-35}$) in C6 glial cells. The treatment of $A{\beta}_{25-35}$ to C6 glial cells generated high levels of reactive oxygen species(ROS) and declined cell viability. However, production of ROS was decreased by the treatment of ECE. In addition, the cell viability was significantly increased at each concentration(10, 25, $50{\mu}g/ml$) as dose-dependent manner. The Alzheimer's disease-related protein expressions in $A{\beta}_{25-35}$-treated C6 glial cells were analyzed. The ECE treatment inhibited expression of amyloid precursor protein(APP), C-terminal fragment-${\beta}(CTF-{\beta})$, ${\beta}$-site APP cleaving enzyme(BACE), phosphorylated tau(p-tau) proteins in C6 glial cells induced by $A{\beta}_{25-35}$. The present study indicated that ECE has strong radical scavenging activity and neuroprotective effect through attenuating oxidative stress.
Keywords
Engelhardtia chrysolepis; C6 glial cell; Reactive oxygen species; Oxidative stress; Radical scavenging; Amyloid $beta_{25-35}$;
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